Functional studies based on the structure of brain water channel, aquaporin-4

• Project/Area Number: 23570191
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Nagoya University (2012-2013); Kyoto University (2011)
• Principal Investigator: HIROAKI Yoko 名古屋大学, 細胞生理学研究センター, 特任准教授 (80378588)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 膜蛋白質 / アクアポリン-４ / 電子線結晶構造解析 / アクアポリン-4 / アクアポリン－４

Research Abstract

AQP4 revealed in the brain at predominance is highly preserved between species. In order to understand the physiological function of AQP4 based on structural analysis, the astrocyte from mice including genetically engineered mice expressing AQP4 without adhesive function were comprehensively analyzed on mRNA as well as a protein level. The culture procedure was established that 90% of the cultured cells showed positive reaction to the astrocyte-markers, such as GFAP. Microarray analyses showed reduction of various kinases but increase of adhesion molecules in the genetically engineered mice compared with those in WT ones. A cytoskeleton related factors and integrin family proteins were found to be decreased by mass spectrometry.
Because of devotion duty caused by my new affiliation, this project has to regrettably be stopped at the middle of the last fiscal year, while this project is interesting.

Research Products

• [Journal Article] Two alternative conformations of a voltage-gated sodium channel. (2013)
  • Author(s): CJ. Tsai, K. Tani, K. Irie, Y. Hiroaki, T. Shimomura, DG. McMillan, GM. Cook, GFX. Schertler, Y. Fujiyoshi and XD. Li
  • Journal Title: J. Mol. Biol. Volume: 425 Issue: 22 Pages: 4074-4088
  • DOI: 10.1016/j.jmb.2013.06.036
  • Peer Reviewed
• [Journal Article] Electron tomographic analysis of gap junctions in lateral giant fibers of crayfish (2011)
  • Author(s): Ohta, Y., Nishikawa, K., Hiroaki, Y. and Fujiyoshi, Y.
  • Journal Title: J. Struct. Biol. Volume: 175(1) Issue: 1 Pages: 49-61
  • DOI: 10.1016/j.jsb.2011.04.002
• [Remarks]
  • URL: http://www.cespi.nagoya-u.ac.jp/
• [Remarks] 細胞生理学研究センター
  • URL: http://www.cespi.nagoya-u.ac.jp/