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NMR analysis of amyloid beta-oligomer by using molecular chaperone encapsulation system.

Research Project

Project/Area Number 23570192
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionKyoto University

Principal Investigator

MASARU Hoshino  京都大学, 薬学研究科(研究院), 准教授 (70304053)

Co-Investigator(Renkei-kenkyūsha) IKEGAMI Takahisa  大阪大学たんぱく質研究所, 准教授 (20283939)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsフォールディング / 分子シャペロン / シャペロニン / 核磁気共鳴 / アミロイドβ / GroE / NMR / アミロイド / 可溶性オリゴマー / NMR / 構造解析
Research Abstract

Nuclear magnetic resonance spectroscopy is a powerful tool for the analysis of protein structure and dynamics in solution. This technique is, however, not able to analyze aggregated proteins and proteins which is in equilibrium in association and dissociation. To circumvent this major disadvantage, it is effective to encapsulate the target protein into the 'cage' composed of molecular chaperone from Escherichia coli. As a first step of the development of molecular chaperone encapsulating system, the long term stability of the molecular chaperone capsule was analyzed by using a green fluorescence protein as a model substrate.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (30 results)

All 2014 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 8 results) Presentation (18 results) (of which Invited: 2 results) Remarks (3 results)

  • [Journal Article] Evaluation of the stability of an SR398/GroES chaperonin complex2014

    • Author(s)
      S. Ishino, Y. Kawata, T. Ikegami, K. Matsuzaki & M. Hoshino.
    • Journal Title

      J. Biochemistry

      Volume: 155 (5) Issue: 5 Pages: 295-300

    • DOI

      10.1093/jb/mvu009

    • NAID

      40020056292

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of the stability of an SR398/GroES chaperonin complex.2014

    • Author(s)
      S. Ishino, Y. Kawata, T. Ikegami, K. Matsuzaki, M. Hoshino
    • Journal Title

      J. Biochem.

      Volume: unknown

    • NAID

      40020056292

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Interaction between soluble Abeta-(1-40) monomer and Abeta-(1-42) fibrils probed by paramagnetic relaxation enhancement.2013

    • Author(s)
      T. Yamaguchi, K. Matsuzaki & M. Hoshino
    • Journal Title

      FEBS Lett.

      Volume: 587 Issue: 6 Pages: 620-624

    • DOI

      10.1016/j.febslet.2013.02.008

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] GM1 cluster mediates formation of toxic Abeta fibrils by providing hydrophobic environments.2012

    • Author(s)
      S. Fukunaga, H. Ueno, T. Yamaguchi, Y. Yano, M. Hoshino & K. Matsuzaki
    • Journal Title

      Biochemistry

      Volume: 51 Issue: 41 Pages: 8125-8131

    • DOI

      10.1021/bi300839u

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Interaction between isolated transcriptional activation domains of Sp1 revealed by heteronuclear magnetic resonance.2012

    • Author(s)
      N. Hiramatsu, E. Hibino, K. Matsuzaki, J. Kuwahara & M. Hoshino
    • Journal Title

      Protein Sci.

      Volume: 21 Issue: 10 Pages: 1481-1488

    • DOI

      10.1002/pro.2137

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structure-Activity Relationship Study of a CXC Chemokine Receptor Type 4 Antagonist, FC131, Using a Series of Alkene Dipeptide Isosteres2012

    • Author(s)
      Kobayashi K., et al
    • Journal Title

      Journal of Medicinal Chemistry

      Volume: (in press) Issue: 6 Pages: 2746-2757

    • DOI

      10.1021/jm2016914

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mechanism of amyloid beta-protein aggregation mediated by GM1 ganglioside clusters.2011

    • Author(s)
      K. Ikeda, T. Yamaguchi, S. Fukunaga, M. Hoshino & Katsumi Matsuzaki
    • Journal Title

      Biochemistry

      Volume: 50 Issue: 29 Pages: 6433-6440

    • DOI

      10.1021/bi200771m

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transient formation of intermediate conformational states of amyloid-beta peptide revealed by heteronuclear magnetic resonance spectroscopy.2011

    • Author(s)
      T. Yamaguchi, K. Matsuzaki & M. Hoshino
    • Journal Title

      FEBS Lett.

      Volume: 585 Issue: 7 Pages: 1097-1102

    • DOI

      10.1016/j.febslet.2011.03.014

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ganglioside-mediated aggregation of amyloid beta-proteins (Abeta): Comparison between Abeta-(1-40) and Abeta-(1-42).2011

    • Author(s)
      M. Ogawa, M. Tsukuda, T. Yamaguchi, K. Ikeda, T. Okada, Y. Yano, M. Hoshino & K. Matsuzaki
    • Journal Title

      J. Neurochem.

      Volume: 116 Issue: 5 Pages: 851-857

    • DOI

      10.1111/j.1471-4159.2010.06997.x

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 溶液NMRによるアミロイドβペプチドの相互作用解析2013

    • Author(s)
      星野大
    • Organizer
      日本薬学会北陸支部特別講演会
    • Place of Presentation
      富山大学杉谷キャンパス
    • Year and Date
      2013-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] アミロイドβペプチドの線維形成機構の解析2013

    • Author(s)
      星野大
    • Organizer
      R-GIROプロジェクトセミナー「蛋白質のフォールディングおよびフォールディング病発祥機構の解明のための統合研究」
    • Place of Presentation
      立命館大学生命科学部・薬学部
    • Year and Date
      2013-03-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 転写因子Sp1とTAF4の天然変性領域を介した相互作用の解析2013

    • Author(s)
      星野大
    • Organizer
      新学術領域研究「天然変性タンパク質の分子認識機構と機能発現」領域会議
    • Place of Presentation
      ひだホテルプラザ
    • Related Report
      2013 Final Research Report
  • [Presentation] Revisiting the stability of an SR398/GroES chaperonin complex2013

    • Author(s)
      S. Ishino, K. Matsuzaki & M. Hoshino.
    • Organizer
      第13回日本蛋白質科学会年会
    • Place of Presentation
      とりぎん文化会館
    • Related Report
      2013 Final Research Report
  • [Presentation] 転写因子Sp1とTAF4の天然変性領域を介した相互作用の解析2013

    • Author(s)
      日比野絵美,桑原淳,松崎勝巳,星野大
    • Organizer
      第13回日本蛋白質科学会年会
    • Place of Presentation
      とりぎん文化会館
    • Related Report
      2013 Final Research Report
  • [Presentation] Interaction between transcriptional activation domains of Sp1 and TAF4 examined by heteronuclear magnetic resonance2013

    • Author(s)
      E. Hibino, J. Kuwahara, K. Matsuzaki & M. Hoshino.
    • Organizer
      新学術領域研究「天然変性タンパク質の分子認識機構と機能発現」第2回国際シンポジウム
    • Place of Presentation
      理化学研究所横浜研究所交流棟ホール
    • Related Report
      2013 Final Research Report
  • [Presentation] 転写因子 Sp1 と TAF4 の天然変性領域を介した相互作用の解析2013

    • Author(s)
      星野 大9月3日~5日,ひだホテルプラザ
    • Organizer
      新学術領域研究「天然変性タンパク質の分子認識機構と機能発現」領域会議
    • Place of Presentation
      ひだホテルプラザ
    • Related Report
      2013 Annual Research Report
  • [Presentation] Revisiting the stability of an SR398/GroES chaperonin complex.2013

    • Author(s)
      S. Ishino, K. Matsuzaki & M. Hoshino
    • Organizer
      第13回日本蛋白質科学会年会
    • Place of Presentation
      とりぎん文化会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 転写因子 Sp1 と TAF4 の天然変性領域を介した相互作用の解析2013

    • Author(s)
      日比野 絵美、桑原 淳、松崎 勝巳、星野 大
    • Organizer
      第13回日本蛋白質科学会年会
    • Place of Presentation
      とりぎん文化会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] アミロイドβペプチドの線維形成機構の解析2013

    • Author(s)
      星野 大
    • Organizer
      R-GIRO プロジェクトセミナー「蛋白質のフォールディングおよびフォールディング病発祥機構の解明のための統合研究」
    • Place of Presentation
      立命館大学 生命科学部・薬学部 びわこくさつキャンパス(滋賀県)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Interaction between transcriptional activation domains of Sp1 and TAF4 examined by heteronuclear magnetic resonance.2013

    • Author(s)
      Emi Hibino, Jun Kuwahara, Katsumi Matsuzaki & Masaru Hoshino
    • Organizer
      新学術領域研究「天然変性タンパク質の分子認識機構と機能発現」 第2回国際シンポジウム
    • Place of Presentation
      理化学研究所 横浜研究所交流棟ホール(神奈川県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 分子シャペロンカプセル化システムの構築2012

    • Author(s)
      星野大
    • Organizer
      大阪大学蛋白質研究所セミナー「分子シャペロンの機能発現の新展開と細胞制御」
    • Place of Presentation
      大阪大学蛋白質研究所
    • Related Report
      2013 Final Research Report
  • [Presentation] グルタミンリッチ型転写活性化ドメインの相互作用解析2012

    • Author(s)
      星野大
    • Organizer
      新学術領域研究「天然変性タンパク質の分子認識機構と機能発現」領域会議
    • Place of Presentation
      北広島クラッセホテル
    • Related Report
      2013 Final Research Report
  • [Presentation] 分子シャペロンカプセル化システムの構築2012

    • Author(s)
      星野 大
    • Organizer
      大阪大学蛋白質研究所セミナー「分子シャペロンの機能発現の新展開と細胞制御」
    • Place of Presentation
      大阪大学蛋白質研究所(大阪府)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 転写因子Sp1の転写活性化ドメイン間相互作用の解明2011

    • Author(s)
      平松直子,日比野絵美,松崎勝巳,桑原淳,星野大
    • Organizer
      第9回次世代を担う若手のためのフィジカル・ファーマフォーラム
    • Place of Presentation
      ホテル箱根アカデミー
    • Related Report
      2013 Final Research Report
  • [Presentation] 転写因子Sp1の転写活性化ドメイン間相互作用の解明2011

    • Author(s)
      平松直子,日比野絵美,松崎勝巳,桑原淳,星野大
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      ホテル阪急エキスポパーク
    • Related Report
      2013 Final Research Report
  • [Presentation] 転写因子 Sp1 の転写活性化ドメイン間相互作用の解明2011

    • Author(s)
      平松 直子、日比野 絵美、松崎 勝巳、桑原 淳、星野 大
    • Organizer
      次世代を担う若手のためのフィジカル・ファーマフォーラム
    • Place of Presentation
      ホテル箱根アカデミー(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Interaction between isolated transcriptional activation domains of Sp1 revealed by heteronuclear magnetic resonance.2011

    • Author(s)
      Naoko Hiramatsu, Emi Hibino, Katsumi Matsuzaki, Jun Kuwahara and Masaru Hoshino
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.pharm.kyoto-u.ac.jp/yakkai/

    • Related Report
      2013 Final Research Report
  • [Remarks] 京都大学大学院・薬学研究科・薬品機能解析学分野

    • URL

      http://www.pharm.kyoto-u.ac.jp/yakkai/

    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.pharm.kyoto-u.ac.jp/yakkai/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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