| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Research Abstract |
There are many different functions even in a structurally homologous proteins. It is not clear how they realize these divergent functions on their same protein backbone. To evaluate these molecular divergent mechanism, two different PYP proteins classified as PAS domain proteins have been investigated about their molecular mechanism for structural changes and protein-protein interaction. As the results, (1) solution structure of the interaction protein for PYP has been identified. And then complex formation of this protein and PYP has been successfully identified. This complex was characteristic ring shape composed by hetero octamer. (2) a surface residue on the PYP has been identified as an interaction site. (3) X-ray crystal analysis for this PYP suggested that structural change was introduced around the interaction residue.
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