Constructing artificial rotors for rotary molecular motor F1(V1)-ATPase
| Project/Area Number |
23570199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Osaka Medical College |
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Principal Investigator |
FURUIKE Shou 大阪医科大学, 医学部, 助教 (60392875)
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| Co-Investigator(Renkei-kenkyūsha) |
YOKOYAMA Ken 京都産業大学, 総合生命科学部, 教授 (70271377)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Keywords | 1分子観察 / ATP合成酵素 / 回転トルク / 回転分子モーター / 光学顕微鏡 / 分子モーター / ATP加水分解 / 1分子計測 / 生物物理 |
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| Research Abstract |
F1(V1)-ATPase is an ATP-driven rotary molecular motor. In view of what designed rotor can rotate, we have investigated which specific regions in the rotor contribute for the rotation. The mutant, in which only the below slight portion was left within contact regions between rotor axle and stator, rotated at half speed and generated half rotational torque relative to wild-type. Together with previous studies, no residues in the rotor are essential for the rotation. An essential property of the rotor may be just the stick-like structure, or any regions (residues) of rotor may contribute for the rotation.
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Report
(4 results)
Research Products
(27 results)
