The differential analyses of tRNA splicing processes among the three domains of life

• Project/Area Number: 23570208
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Gifu University
• Principal Investigator: YOKOGAWA Takashi 岐阜大学, 工学部, 教授 (90242304)
• Co-Investigator(Renkei-kenkyūsha): OHNO Satoshi 岐阜大学, 工学部, 助教 (10345796)
• Research Collaborator: NOMURA Yuichiro 岐阜大学, 大学院工学研究科, 大学院生 ; KUNO Miyuki 岐阜大学, 大学院工学研究科, 大学院生
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: tRNA / tRNAリガーゼ / スプライシング / 古細菌 / EndA / RtcB / イントロン / 低分子環状RNA / アーケオシン / ArcTGT / BHBモチーフ

Research Abstract

In Archaea and Eucarya, some tRNA genes contain introns. Since the precursor tRNA (pre-tRNA) is not functional in protein biosynthesis, exact removal of introns must be essential. Recently, it was found that the RtcB catalyzes the ligation of exons derived from the pre-tRNA. RtcB also seems to catalyze the circulation of intron from pre-tRNA. In fact, we found the circularized intron in methanogenic archaeon, Methanosarcina acetivorans, cells. Whereas the circularized intron has any function or not, we believe it would be functional because of the following facts. First, the circularized intron was stable in M. acetivorans cells. Second, RtcB even exists in bacterial cells that any tRNA gene does not contain intron. In future, we would like to discover the functions of small circularized RNAs.

Research Products

• [Journal Article] A simple system for expression of proteins containing 3-azidotyrosine at a pre-determined site in Escherichia coli. (2013)
  • Author(s): Ikeda-Boku, A., Ohno, S., Hibino, Y., Yokogawa, T., Hayashi, N., Nishikawa, K.
  • Journal Title: J. Biochem. Volume: 153 Issue: 3 Pages: 317-326
  • DOI: 10.1093/jb/mvs153
  • NAID: 40019589671
  • Peer Reviewed
• [Journal Article] Protein fishing using magnetic nanobeads containing calmodulin site-specifically immobilized via an azido group. (2013)
  • Author(s): Ikeda-Boku, A., Kondo, K., Ohno, S., Yoshida, E., Yokogawa, T., Hayashi, N., Nishikawa, K.
  • Journal Title: J. Biochem. Volume: 154 Issue: 2 Pages: 159-165
  • DOI: 10.1093/jb/mvt038
  • NAID: 40019731100
  • Peer Reviewed
• [Journal Article] Purification and comparison of native and recombinant tRNA-guanine transglycosylases from Methanosarcina acetivorans. (2013)
  • Author(s): Nomura, Y, Onda, Y, Ohno, S, Taniguchi, H, Ando, K, Oka, N, Nishikawa, K, Yokogawa, T
  • Journal Title: Protein Expr. Purif. Volume: 88 Issue: 1 Pages: 13-19
  • DOI: 10.1016/j.pep.2012.11.009
  • Peer Reviewed
• [Presentation] Methanosarcina acetivorans由来tRNA-guanine transglycosylaseにおける天然型および組換え型酵素の精製とその比較 (2013)
  • Author(s): 能村友一朗
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山市
• [Presentation] tRNAのスプライシングによって生じる環状RNAはM. acetivorans細胞内で安定に存在している (2013)
  • Author(s): 久野美由紀
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山市
• [Presentation] Methanosarcina acetivoransのイントロン含有tRNA前駆体と結合するタンパク質群の解析 (2011)
  • Author(s): 横川隆志
  • Organizer: 24回Archaea研究会
  • Place of Presentation: 鶴岡市
  • Year and Date: 2011-09-03
• [Presentation] Methanosarcina acetivoransのイントロン含有tRNA前駆体と結合するタンパク質群の解析 (2011)
  • Author(s): 横川隆志
  • Organizer: 24回Archaea研究会
  • Place of Presentation: 鶴岡