| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
T4 adsorption to E. coli K12 strain depends on the presence of OmpC, but T4 can adsorb to B strain independently of OmpC. Using K12 mutants defective in various genes required for LPS synthesis, we systematically analyzed the adsorption of T4 phage to these mutants dependent on or independent of OmpC. Furthermore, we isolated T4 mutants which can adsorb to B strain but not to K12 strain or vice versa. These results strongly suggest that T4 has two different mechanisms for adsorption to host cells. In addition, present study identified specific structures in OmpC and the distal tip of T4 long tail fiber, required for the recognition of OmpC by T4.
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