| Project/Area Number |
23570216
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Yasuda Women's University |
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Principal Investigator |
INOUE Sachie 安田女子大学, 薬学部, 教授 (60159978)
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| Co-Investigator(Kenkyū-buntansha) |
AKAGI Reiko 安田女子大学, 薬学部, 教授 (50150967)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 熱ショック因子 / ストレス応答 / ヘムオキシゲナーゼ / 発現制御 / 生体防御 / 小Maf転写因子 / 小Ｍａｆ転写因子 / 転写制御 / 酸化ストレス / 熱ショック転写因子 |
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| Research Abstract |
Heme-degrading enzyme, heme oxygenase-1 (HO-1), plays a role protecting cells against to oxidative stress. The HO-1 is also known as heat shock protein 32 (Hsp32), however, the heat inducible HO-1 is different in species, cell lines, and culture conditions. In this study, we examined the role of heat shock factor 1 (HSF1) on the regulation of HO-1 expression by using wild-type and HSF1-null mouse embryonic fibroblast (MEF). Our results showed that HSF1 suppressed heme-induced HO-1 expression in normal growth condition and facilitated heme-induced HO-1 expression during heat shock. In conclusion, HSF1 protect cells by not only inducing the expression of Hsps under heat stress condition but also fine controlling the expression of HO-1 under various stress conditions.
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