Analysis of storage mRNP components

• Project/Area Number: 23570218
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MATSUMOTO Ken 独立行政法人理化学研究所, 吉田化学遺伝学研究室, 専任研究員 (60222311)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 翻訳 / 蛋白質 / 核酸

Research Abstract

To elucidate regulatory mechanisms involved in translation and stability of mRNA, it is essential to understand functions of mRNP components. In this work, we further characterized mRNP components RAP55/Lsm14 and P100 that we previously identified. To obtain proteins that functionally interact with RAP55, we performed a screening based on the co-localization with RAP55, effects of the overexpression on the formation of mRNP granules or the disappearance of RAP55 from mRNP granules. In addition, to understand the mechanism with which P100 disappears from oocytes during oocyte maturation, we prepared several point mutants and analyzed their stability.

Research Products

• [Journal Article] FETタンパク質ファミリーの疾患との関わり (2014)
  • Author(s): 松本健
  • Journal Title: 生化学 Volume: 86 Pages: 274-280
  • NAID: 40020096302
• [Journal Article] EWS represses cofilin 1 expression by inducing nuclear retention of cofilin 1 mRNA. (2014)
  • Author(s): Huang, L., Kuwahara, I., and Matsumoto, K.
  • Journal Title: Oncogene Volume: 印刷中 Issue: 23 Pages: 2995-3003
  • DOI: 10.1038/onc.2013.255
  • Peer Reviewed
• [Journal Article] Silencing the YB-1 gene inhibits cell migration in gastric cancer in vitro. (2013)
  • Author(s): Guo, T. T., Yu, Y.-N., Yip, G. W.-C., Matsumoto, K., and Bay, B.-H.
  • Journal Title: Anat. Rec. Volume: 296 Issue: 6 Pages: 891-898
  • DOI: 10.1002/ar.22702
  • Peer Reviewed
• [Journal Article] PRMT1 is required for RAP55 to localize to processing bodies. (2012)
  • Author(s): Matsumoto, K., Nakayama, H., Yoshimura, M., Masuda, A., Dohmae, N., Matsumoto, S., and Tsujimoto, M.
  • Journal Title: RNA Biology Volume: 9 Issue: 5 Pages: 610-623
  • DOI: 10.4161/rna.19527
  • Peer Reviewed
• [Journal Article] アフリカツメガエル卵母細胞内の翻訳抑制因子P100に結合する母性mRNAの探索 (2012)
  • Author(s): 中村依子, 中井雄治, 松本健
  • Journal Title: 日大口腔科学 Volume: 38 Pages: 33-36
  • NAID: 40019489614
  • Peer Reviewed
• [Journal Article] Surmounting chemoresistance by targeting the Y-Box Binding Protein-1 (2012)
  • Author(s): Inoue, I., Matsumoto, K., Yu, Y., and Bay, B.-H.
  • Journal Title: Anat. Rec. Volume: 295 Issue: 2 Pages: 215-222
  • DOI: 10.1002/ar.22401
  • Peer Reviewed
• [Journal Article] PRAS40 is a functionally critical target for EWS repression in Ewing's sarcoma (2012)
  • Author(s): Huang, L., Nakai, Y., Kuwahara, I., and Matsumoto, K.
  • Journal Title: Cancer Res. Volume: 72 Issue: 5 Pages: 1260-1269
  • DOI: 10.1158/0008-5472.can-11-2254
  • Peer Reviewed
• [Journal Article] YB-1, Y-box binding protein-1, is a porter to lead influenza virus ribonucleoprotein complexes to microtubules. (2012)
  • Author(s): Kawaguchi, A., Matsumoto, K., and Nagata, K.
  • Journal Title: Journal of Virology Volume: 86 Issue: 20 Pages: 11086-11095
  • DOI: 10.1128/jvi.00453-12
  • Peer Reviewed
• [Journal Article] The NS1 protein of influenza A virus interacts with cellular processing bodies (P-bodies) and stress granules through RNA-associated protein 55 (RAP55) during virus infection. (2012)
  • Author(s): 42. Mok, B. W.-Y., Song, W., Wang, P., Tai, H., Chen, Y.-X., Zheng, M., Wen, X., Lau, S.-Y., Wu, W. L., Matsumoto, K., Yuen, K.-Y., and Chen, H.
  • Journal Title: Journal of Virology Volume: 86 Issue: 23 Pages: 12695-12707
  • DOI: 10.1128/jvi.00647-12
  • Peer Reviewed
• [Presentation] YBAP1によるYB-1の活性調節 (2014)
  • Author(s): 松本健, 吉村麻美
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
  • Year and Date: 2014-03-28
• [Presentation] RNA結合蛋白質EWSによるmRNAの転写後調節 (2013)
  • Author(s): 黄琳, 中井雄治, 桑原いく, 松本健
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-29
• [Presentation] EWS represses protein expression by inducing nuclear retention of its mRNA targets. The 2^<nd> Meeting of RNA and Biofunctions-Asia Study (2013)
  • Author(s): Huang, L., and Matsumoto, K.
  • Organizer: RNA Biofunctions and Viruses
  • Place of Presentation: Hakata, Fukuoka
  • Year and Date: 2013-01-10
• [Presentation] EWS induces the nuclear retention of its target mRNAs and represses their protein expression in Ewing's sarcoma (2012)
  • Author(s): Huang, L., Kuwahara, I., and Matsumoto, K.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] Y-box binding protein-1 as a molecular target for potentiating chemotherapeutic drugs (2012)
  • Author(s): Yu, Y.-N., Inoue, I., Guo, T., Ng, C.-T., Wong, J.-Y., Matsumoto, K., Yip, G. W., and Bay, B.-H.
  • Organizer: The 24^<th> EORTC-NCI-AACR Symposium on Molecular Targets and Cancer Therapeutics
  • Place of Presentation: Dublin, Ireland
  • Year and Date: 2012-11-09
• [Presentation] ツメガエルの卵成熟に関与するmRNP構成因子P100には何が結合するのか (2012)
  • Author(s): 中村依子, 中井雄治, 松本健, 城座映明
  • Organizer: XCIJ-All/MA研究集会
  • Place of Presentation: 東京
  • Year and Date: 2012-07-14
• [Presentation] アフリカツメガエル卵母細胞特異的蛋白質P100に結合する卵成熟関与遺伝子 (2012)
  • Author(s): 中村依子, 中井雄治, 松本健, 城座映明
  • Organizer: 平成24年度日本生化学会関東支部例会
  • Place of Presentation: 前橋
  • Year and Date: 2012-06-23
• [Presentation] RAP55 is important for the assembly, size and composition of cytoplasmic mRNP granules (2012)
  • Author(s): Matsumoto, K., Minami, M., Minami, Y., Matsumoto, S.
  • Organizer: The 10^<th> Asia Pacific Microscopy Conference
  • Place of Presentation: Perth, Australia
  • Year and Date: 2012-02-07
• [Presentation] RAP55 is important for the assembly, size and composition of cytoplasmic mRNP granules. (2012)
  • Author(s): Matsumoto, K., Minami, M., Minami, Y., Matsumoto, S.
  • Organizer: The 10th Asia Pacific Microscopy Conference(招待講演)
  • Place of Presentation: Perth, Australia
• [Presentation] Functional repression of an EWS-bound mRNA in Ewing's sarcoma (2011)
  • Author(s): Huang, L., Nakai, Y., Kuwahara, I., and Matsumoto, K.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] Identification of EWS-bound mRNAs and their functional role in Ewing's sarcoma cells (2011)
  • Author(s): Huang, L., Nakai, Y., Kuwahara, I., and Matsumoto, K.
  • Organizer: NTU-JST Joint Meeting on RNA and Biofunctions-Asia Studies
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2011-11-11
• [Presentation] TNRC6 proteins interact with multiple Argonautes to form large RNP aggregates (2011)
  • Author(s): Takimoto, K., Matsumoto, K., Yamashita, S., Muramatsu, R., Yokoyama, S., and Wakiyama, M.
  • Organizer: RNA 2011: 16th Annual Meeting of the RNA Society in conjunction with 13th Annual Meeting of the RNA Society of Japan
  • Place of Presentation: Kyoto
  • Year and Date: 2011-06-15
• [Presentation] Regulation of the activities of YB-1 by YBAP1 (2011)
  • Author(s): Matsumoto, K., and Yoshimura, M.
  • Organizer: RNA 2011: 16th Annual Meeting of the RNA Society in conjunction with 13th Annual Meeting of the RNA Society of Japan
  • Place of Presentation: Kyoto
  • Year and Date: 2011-06-15
• [Presentation] Regulation of the activities of YB-1 by YBAP1/p32 (2011)
  • Author(s): Matsumoto, K. and Yoshimura, M.
  • Organizer: RNA 2011
  • Place of Presentation: Kyoto
• [Presentation] Functional repression of an EWS-bound mRNA in Ewing’s sarcoma. (2011)
  • Author(s): Huang, L., Nakai, Y., Kuwahara, I., and Matsumoto, K.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] YBAP1によるYB-1の活性調節
  • Author(s): 松本 健、吉村麻美
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
• [Presentation] EWS represses protein expression by inducing nuclear retention of its mRNA targets.
  • Author(s): Huang, L. and Matsumoto, K.
  • Organizer: The 2nd Meeting of RNA and Biofunctions-Asia Study: RNA Biofunctions and Viruses
  • Place of Presentation: ホテルレオパレス博多（福岡県）
• [Presentation] アフリカツメガエル卵母細胞特異的蛋白質P100に結合する卵成熟関与遺伝子
  • Author(s): 中村依子、中井雄治、松本 健、城座映明
  • Organizer: 平成24年度日本生化学会関東支部例会
  • Place of Presentation: 群馬大学（前橋市）
• [Presentation] EWS induces the nuclear retention of its target mRNAs and represses their protein expression in Ewing’s sarcoma.
  • Author(s): Huang, L., Kuwahara, I., and Matsumoto, K.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] YB-1, Y-box binding protein-1, is a porter to lead influenza virus ribonucleoprotein complexes to microtubules
  • Author(s): Kawaguchi, A., Matsumoto, K. and Nagata, K.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] RNA結合蛋白質EWSによるmRNAの転写後調節
  • Author(s): 黄 琳、中井雄治、桑原いく、松本 健
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜
• [Remarks]
  • URL: http://www.riken.jp/matsumok/index.html
• [Remarks]
  • URL: http://www.riken.jp/matsumok/