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Regulation of cellular functions by highly evolved two chaperonins

Research Project

Project/Area Number 23570220
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionAkita University

Principal Investigator

KUBOTA HIROSHI  秋田大学, 工学(系)研究科(研究院), 教授 (80332724)

Co-Investigator(Renkei-kenkyūsha) NAGATA Kazuhiro  京都産業大学, 総合生命科学部, 教授 (50127114)
KINJO Masataka  北海道大学, 先端生命科学研究院, 教授 (70177971)
KITAMURA Akira  北海道大学, 先端生命科学研究院, 助教 (10580152)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords分子シャペロン / フォールディング / 複合体形成 / ATPase / CCT / HSP60 / 反応サイクル / タンパク質相互作用 / 構造変化 / 機能制御 / 基質特異性 / ATP
Research Abstract

Chaperonins are a group of molecular chaperone that assists in protein folding in the cell. In this project, we analyzed two eukaryotic chaperonins: cytosolic chaperonin CCT and mitochondrial chaperonin HSP60. In the study of CCT, we found that CCT possesses GTPase activity in addition to the ATPase activity. In the study of HSP60, we found that football-like complex plays an important role in the function of HSP60 after formation of the football-like complex from single-ring complex via double-ring complex. These results contain important findings to understand chaperonin functions.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (25 results)

All 2014 2013 2012 2011

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (14 results)

  • [Journal Article] ATPase activity and ATP-dependent conformational change in the co-chaperone HOP.2014

    • Author(s)
      Yamamoto S, Subedi GP, Hanashima S, Satoh T, Otaka M, Wakui H, Sawada KI, Yokota SI, Yamaguchi Y, Kubota H, Itoh H.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 289 Issue: 14 Pages: 9880-9886

    • DOI

      10.1074/jbc.m114.553255

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serum heat shock protein 47 levels are elevated in acute interstitial pneumonia.2014

    • Author(s)
      Kakugawa, T., Yokota, S.I., Ishimatsu, Y., Hayashi, T., Nakashima, S., Hara, S., Sakamoto, N., Kubota, H., Mine, M., Matsuoka, Y., Mukae, H., Nagata, K., and Kohno, S.
    • Journal Title

      BMC Pulm. Med.

      Volume: 14 Issue: 1 Pages: 48-48

    • DOI

      10.1186/1471-2466-14-48

    • NAID

      120006986596

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serum heat shock protein 47 levels are elevated in acute exacerbation of idiopathic pulmonary fibrosis2013

    • Author(s)
      Kakugawa, T., Yokota, S.I., Ishimatsu, Y., Hayashi. T., Nakashima, S., Hara, S., Sakamoto, N., Kubota, H., Mine, M., Matsuoka, Y., Mukae, H., Nagata, K., and Kohno, S.
    • Journal Title

      Cell Stress Chaperones

      Volume: 18 Issue: 5 Pages: 581-590

    • DOI

      10.1007/s12192-013-0411-5

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prefoldin protects neuronal cells from polyglutamine toxicity by preventing aggregation formation.2013

    • Author(s)
      Tashiro E, Zako T, Muto H, Itoo Y, Sorgjerd K, Terada N, Abe A, Miyazawa M, Kitamura A, Kitaura H, Kubota H, Maeda M, Momoi T, Iguchi-Ariga SM, Kinjo M, Ariga H.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 288 Issue: 27 Pages: 19958-19972

    • DOI

      10.1074/jbc.m113.477984

    • NAID

      120005323603

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serum heat shock protein 47 levels in patients with drug-induced lung disease.2013

    • Author(s)
      Kakugawa, T., Yokota, S., Ishimatsu, Y., Hayashi, T., Nakashima, S., Hara, S., Sakamoto, N., Kubota, H., Mine, M., Y., Matsuoka, Y., Mukae, H., Nagata, K., and Kohno, S.
    • Journal Title

      Respir. Res.

      Volume: 14 Issue: 1 Pages: 133-133

    • DOI

      10.1186/1465-9921-14-133

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serum heat shock protein 47 levels are elevated in acute exacerbation of idiopathic pulmonary fibrosis.2013

    • Author(s)
      Kakugawa, T., Yokota, S.I., Ishimatsu, Y., Hayashi. T., Nakashima, S., Hara, S., Sakamoto, N., Kubota, H., Mine, M., Matsuoka, Y., Mukae, H., Nagata, K., and Kohno, S.
    • Journal Title

      Cell Stress Chaperones

      Volume: 未定

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Multipotent stem cells are effectively collected from adult human cheek skin.2012

    • Author(s)
      Yoshikawa, K., Naitoh, M., Kubota, H., Ishiko, T., Aya, R., Yamawaki, S. and Suzuki, S.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 431 Issue: 1 Pages: 104-110

    • DOI

      10.1016/j.bbrc.2012.12.069

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pirfenidone inhibits TGF-beta1-induced over-expression of collagen type I and heat shock protein 47 in A549 cells.2012

    • Author(s)
      Hisatomi, K., Mukae, H., Sakamoto, N., Ishimatsu, Y., Kakugawa, T., Hara, S., Fujita, H., Nakamichi, S., Oku, H., Urata, Y., Kubota, H., Nagata, K. and Kohno, S.
    • Journal Title

      BMC Pulm. Med.

      Volume: 12 Issue: 1

    • DOI

      10.1186/1471-2466-12-24

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cytosolic chaperonin CCT possesses GTPase activity2011

    • Author(s)
      Noguchi, et al
    • Journal Title

      Am.J.Mol.Biol.

      Volume: 1 Issue: 03 Pages: 123-130

    • DOI

      10.4236/ajmb.2011.13013

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Protective role of the ubiquitin binding protein Tollip against the toxicity of polyglutamine-expansion proteins2011

    • Author(s)
      Oguro, et al
    • Journal Title

      Neurosci.Lett.

      Volume: 503 Issue: 3 Pages: 234-239

    • DOI

      10.1016/j.neulet.2011.08.043

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protective role of the ubiquitin binding protein Tollip against the toxicity of polyglutamine-expansion proteins2011

    • Author(s)
      Oguro et al.
    • Journal Title

      Neurosci. Lett.

      Volume: 503 Pages: 234-239

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 筋萎縮性側索硬化症原因タンパク質SOD変異体のリソソーム依存的分解2013

    • Author(s)
      田村拓, 山田大介, 久保田広志
    • Organizer
      第8回臨床ストレス応答学会
    • Place of Presentation
      松本市
    • Related Report
      2013 Final Research Report
  • [Presentation] Lysosome-dependent degradation of ALS-linked mutant SOD12013

    • Author(s)
      Daisuke Yamada, Taku Tamura, Hiroshi Kubota
    • Organizer
      International Mini-symposium Protein Folding and Disease
    • Place of Presentation
      秋田市
    • Related Report
      2013 Final Research Report
  • [Presentation] Actin and myosin participate in the quality control of SOD1 aggregates2013

    • Author(s)
      Taku Tamura, Akihiko Yatabe, Koji Ichinohe, Yuko Shimizu, Akira Kitamura, Masataka Kinjo, Hiroshi Kubota
    • Organizer
      International Mini-symposium Protein Folding and Disease
    • Place of Presentation
      秋田市
    • Related Report
      2013 Final Research Report
  • [Presentation] アクチン・ミオシン系は筋萎縮性側索硬化症原因タンパク質SOD1変異体の凝集制御および分解に関与する2013

    • Author(s)
      田村拓, 矢田部暁彦, 一戸康治, 清水佑子, 北村朗, 金城政孝, 久保田広志
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋市
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Aminoglycoside antibiotics suppress the Heat Shock Protein 70 function2013

    • Author(s)
      Yamamoto, S., Yokota, S., Wakui, H., Kubota, H., Itoh, H, and Fujii, N.
    • Organizer
      28th International Congress of Chemotherapy and Infection
    • Place of Presentation
      横浜市
    • Related Report
      2013 Final Research Report
  • [Presentation] Aminoglycoside antibiotics suppress the Heat Shock Protein 70 function.2013

    • Author(s)
      Yamamoto S, Yokota S, Wakui H, Kubota H, Itoh H, Fujii N
    • Organizer
      28th International Congress of Chemotherapy and Infection
    • Place of Presentation
      横浜市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Lysosome-dependent degradation of ALS-linked mutant SOD12013

    • Author(s)
      Daisuke Yamada, Taku Tamura, Hiroshi Kubota
    • Organizer
      International Mini-symposium: Protein Folding and Disease
    • Place of Presentation
      秋田市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Actin and myosin participate in the quality control of SOD1 aggregates2013

    • Author(s)
      Taku Tamura, Akihiko Yatabe, Koji Ichinohe, Yuko Shimizu, Akira Kitamura, Masataka Kinjo, Hiroshi Kubota
    • Organizer
      International Mini-symposium: Protein Folding and Disease
    • Place of Presentation
      秋田市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 筋萎縮性側索硬化症原因タンパク質SOD1変異体のリソソーム依存的分解2013

    • Author(s)
      田村拓、山田大介、久保田広志
    • Organizer
      第8回臨床ストレス応答学会
    • Place of Presentation
      松本市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 筋萎縮性側索硬化症原因タンパク質SOD1変異体の凝集制御および分解おけるアクチンフィラメントの役割2012

    • Author(s)
      田村拓, 矢田部暁彦, 一戸康児, 清水佑子, 北村朗, 金城政孝, 久保田広志
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡市
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Serine Protease-Family Protein HtrA1 is Specifically Up-regulated in Keloid Legions2012

    • Author(s)
      Motoko Naitoh, Toshihiro Ishiko, Satoko Yamawaki, Taku Tamura, Rino Aya, Katsuhiro Yoshikawa, Hiroshi Kubota and Shigehiko Suzuki.
    • Organizer
      第4回世界創傷治癒学会連合会議(WUWHS 2012)
    • Place of Presentation
      横浜市
    • Related Report
      2013 Final Research Report
  • [Presentation] Serine Protease-Family Protein HtrA1 is Specifically Up-regulated in Keloid Legions2012

    • Author(s)
      Motoko Naitoh, Toshihiro Ishiko, Satoko Yamawaki, Taku Tamura, Rino Aya, Katsuhiro Yoshikawa, Hiroshi Kubota and Shigehiko Suzuki.
    • Organizer
      第4回世界創傷治癒学会連合会議(WUWHS 2012)
    • Place of Presentation
      横浜市
    • Related Report
      2012 Research-status Report
  • [Presentation] シャペロニンHsp60のATP、Hsp10依存的なダブルリング化について2011

    • Author(s)
      石田竜一, 久保田広志, 高橋弘樹, 岡俊, 北村朗, 金城政孝, 伊藤英晃
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      大阪市
    • Related Report
      2013 Final Research Report
  • [Presentation] シャペロニンHsp60 のATP、Hsp10 依存的なダブルリング化について2011

    • Author(s)
      石田竜一 他
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      大阪市
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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