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Functional analysis of proteasome activators

Research Project

Project/Area Number 23570221
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

CHIBA Tomoki  筑波大学, 生命環境系, 教授 (00311423)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsタンパク質分解 / 翻訳後修飾 / ストレス応答 / 遺伝子改変動物 / プロテアソーム / ユビキチン / 免疫
Research Abstract

Intracellular protein degradation is highly regulated by the Ubiquitin and Proteasome system (UPS), which plays critical roles in many biological processes such as cell cycle progression, stress response, signal transduction, and so on. The impairment of the UPS is associated with diverse array of diseases such as neurodegenerative diseases, and immune diseases.
Proteasome is a barrel-shaped multisubunit protease complex that consists of latent 20S core particle (CP) and regulatory particles (RPs). The function of CP is regulated by evolutionarily conserved multiple RPs in the cells. However the function and physiological roles of each RPs are not well understood. To reveal the specific function of each RPs, we generated knockout mice of one of the proteasome-associated protein whose function has not yet been elucidated in mammal.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (34 results)

All 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (22 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Myeloma Overexpressed 2. (Myeov2) Regulates L11 Subnuclear Localization through Nedd8 Modification.2013

    • Author(s)
      Manato Ebina, Fuminori Tsuruta, Megumi C. Katoh, Yu Kigoshi, Akie Someya, Tomoki Chiba
    • Journal Title

      PLoS One

      Volume: VoL 8 Issue: 6 Pages: 65285-65285

    • DOI

      10.1371/journal.pone.0065285

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acetylation-Mediated Proteasomal Degradation of Core Histones during DNA Repair and Spermatogenesis2013

    • Author(s)
      Qian MX, Pang Y, Liu CH, Haratake K, Du BY, Ji DY, Wang GF, Zhu QQ, Song W, Yu Y, Zhang XX, Huang HT, Miao S, Chen LB, Zhang ZH, Liang YN, Liu S, Cha H, Yang D, Zhai Y, Komatsu T, Tsuruta F, Li H, Cao C, Li Wei, Li GH, Cheng Y, Chiba T, Wang L, Goldberg AL, Shen Y, Qui XB.
    • Journal Title

      Cell

      Volume: 153 Pages: 1012-1024

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Brap2 Regulates Temporal Control of NF-kB Localization Mediated by Inflammatory Response2013

    • Author(s)
      Takashima 0, Tsuruta F, Kigoshi Y, Nakamura S, Kim J, Katoh MC, Fukuda T, Irie K, Chiba T.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 3 Pages: 58911-58911

    • DOI

      10.1371/journal.pone.0058911

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A method for inducing antigen-specific IgG production by in vitro immunization.2012

    • Author(s)
      Kato M, Yan H, Tsuji NM, Chiba T, Hanyu Y.
    • Journal Title

      J Immunol Methods.

      Volume: 386 Issue: 1-2 Pages: 60-69

    • DOI

      10.1016/j.jim.2012.08.019

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Spermatogenesis arrest caused by conditional deletion of Hsp90a in adult mice.2012

    • Author(s)
      Kajiwara C, Kondo S, Uda S, Dai L, Ichiyanagi T, Chiba T, Ishido S, Koji T and Udono H.
    • Journal Title

      Biology Open (BIO)

      Volume: 1 Issue: 10 Pages: 977-982

    • DOI

      10.1242/bio.2012646

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Smad7-deficient mice show growth retardation with reduced viability2012

    • Author(s)
      Tojo M, Takebe A, Takahashi S, Tanaka K, Imamura T, Miyazono K, Chiba T
    • Journal Title

      J Biochem.

      Volume: (in press) Issue: 6 Pages: 621-631

    • DOI

      10.1093/jb/mvs022

    • NAID

      10031166104

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ubiquitin Ligase Activity of Cul3-KLHL7 Protein Is Attenuated by Autosomal Dominant Retinitis Pigmentosa Causative Mutation2011

    • Author(s)
      Kigoshi Y,Tsuruta F, Chiba T.
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Pages: 33613-33621

    • NAID

      120007137439

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] The Regulation of Nrf2-Keap1 Antioxidant Response by a Novel Cullin Associated Protein2013

    • Author(s)
      Kigoshi Y., Fukuda T., Endo T., Tsuruta F., Chiba T.
    • Organizer
      2013年度日本細胞生物学会
    • Place of Presentation
      名古屋
    • Year and Date
      2013-06-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of a New Binding Partner of KLHL7, a Retinitis Pigmentosa Causative Gene Product2013

    • Author(s)
      Kigoshi Y., Chiba T.
    • Organizer
      2013年日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] A Cullin Related Protein is Involved in CRL Assembly Cycle2013

    • Author(s)
      Fukuda T., Kigoshi Y., Chiba T.
    • Organizer
      2013年日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] The Screening for the Ubiquitination Targets of KLHL7, a Retinitis Pigmentosa Causative Gene Product2013

    • Author(s)
      Endo T., Kigoshi Y., Chiba T.
    • Organizer
      2013年日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] The Regulation of Nrf2-Keap1 Antioxidant Response by a Novel Cullin-like Protein2013

    • Author(s)
      Kigoshi Y., Fukuda T., Endo T., Tsuruta F., Chiba T.
    • Organizer
      The Ubiquitin Meeting
    • Place of Presentation
      Cold Spring Harbor, New York
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of a New Binding Partner of KLHL7, a Retinitis Pigmentosa Causative Gene Product2013

    • Author(s)
      Y. Kigoshi, T. Chiba
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸コンベンションセンター
    • Related Report
      2013 Annual Research Report
  • [Presentation] A Cullin Related Protein is Involved in CRL Assembly Cycle2013

    • Author(s)
      T. Fukuda, Y. Kigoshi, T. Chiba
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸コンベンションセンター
    • Related Report
      2013 Annual Research Report
  • [Presentation] The Screening for the Ubiquitination Targets of KLHL7, a Retinitis Pigmentosa Causative Gene Product2013

    • Author(s)
      T. Endo, Y. Kigoshi, F. Tsuruta, T. Chiba
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸コンベンションセンター
    • Related Report
      2013 Annual Research Report
  • [Presentation] The Regulation of Nrf2-Keap1 Antioxidant Response by a Novel cullin-like Protein2013

    • Author(s)
      Y. Kigoshi, T. Fukuda, T. Endo, F. Tsuruta, T. Chiba
    • Organizer
      Cold Spring Harbor Laboratory Meeting "The Ubiquitin Family"
    • Place of Presentation
      Cold Spring Harbor, NY, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] ストレス条件化における哺乳類Ecm29の機能解析2012

    • Author(s)
      Haratake K., Tsuruta F., Chiba T.
    • Organizer
      2012年日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] An Inhibitory Cullin Positively Regulates the Nrf2 Antioxidant Response2012

    • Author(s)
      Yu Kigoshi, Fuminori Tsuruta, Tomoki Chiba
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2012 Research-status Report
  • [Presentation] MYEOV2 Regulates NEDDylation and Localization of RPL112012

    • Author(s)
      Manato Ebina, Fuminori Tsuruta, Tomoki Chiba
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Characterization of Mammalian Ecm29 under Stress Conditions2012

    • Author(s)
      Kousuke Haratake, Fuminori Tsuruta, Tomoki Chiba
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Loss of USP15 Exhibits Ataxia-like Symptoms in Mice2012

    • Author(s)
      Jaehyun Kim, Fuminori Tsuruta, Tomoki Chiba
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Analysis of a Novel Protein Involved in the Regulation of the Antioxidant Response2012

    • Author(s)
      Yu Kigoshi, Fuminori Tsuruta, Tomoki Chiba
    • Organizer
      5th Japan China Korea Graduate Student Forum
    • Place of Presentation
      Univ. of Tsukuba
    • Related Report
      2012 Research-status Report
  • [Presentation] SCF-Fbl12 controls DNA damage-induced cell cycle arrest2012

    • Author(s)
      Tsuruta F, Chiba T.
    • Organizer
      Keystone Symposia "Ubiquitin Signaling"
    • Place of Presentation
      Whistler Conference Centre, Canada
    • Related Report
      2011 Research-status Report
  • [Presentation] A novel Cullin family protein positively regulates the Nrf2 antioxidant response.2012

    • Author(s)
      Kigoshi Y, Tsuruta F, Chiba T.
    • Organizer
      Keystone Symposia "Ubiquitin Signaling"
    • Place of Presentation
      Whistler Conference Centre, Canada
    • Related Report
      2011 Research-status Report
  • [Presentation] The analysis of proteasome-associated protein, Ecm292011

    • Author(s)
      Haratake K., Uda S., Tsuruta F., Sato A., Udono H., Chiba T.
    • Organizer
      2011年度日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] A novel NEDD8-binding protein modulates NF-kB signaling pathway.2011

    • Author(s)
      Takashima O, Tsuruta F, Ebina M, Kigoshi Y, Nakamura S, Chiba T.
    • Organizer
      1st Bio-Rheumatology International Congress(招待講演)
    • Place of Presentation
      Tokyo Bay Hilton(千葉県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Analysis of proteasome-associated protein, Ecm292011

    • Author(s)
      Haratake K, Sato A, Tsuruta F, Chiba T.
    • Organizer
      The 34th Annual Meeting of the Molecular Biology Society of Japan
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Regulation of protein neddylation by a novel tumor related protein, MYEOV2.2011

    • Author(s)
      Ebina M, Tsuruta F, Chiba T.
    • Organizer
      The 34th Annual Meeting of the Molecular Biology Society of Japan
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] The Regulation of Nrf2-Keap1 Antioxidant Response by a Novel Cullin Associated Protein

    • Author(s)
      Y. Kigoshi, T. Fukuda, T. Endo, F. Tsuruta, T. Chiba
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      ウィンクあいち
    • Related Report
      2013 Annual Research Report
  • [Book] 細胞生物学2012

    • Author(s)
      沼田治,千葉智樹、中野賢太郎、中田和人
    • Total Pages
      203
    • Publisher
      化学同人
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://tchibalab.org

    • Related Report
      2013 Final Research Report
  • [Remarks] 精子形成におけるヒストンの分解機構を解明

    • URL

      http://www.mbs.life.tsukuba.ac.jp

    • Related Report
      2013 Annual Research Report
  • [Remarks] 千葉智樹研究室のHP | 筑波大学

    • URL

      http://tchibalab.org/

    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://tchibalab.org/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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