The mechanism of Nup98-fusion mediated oncogenesis.

• Project/Area Number: 23570228
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: OKA Masahiro 大阪大学, 生命機能研究科, 助教 (40432504)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ヌクレオポリン / 核膜孔 / Nup98 / 白血病 / Hox / ヒストン / がん化 / ヒストン修飾 / 細胞

Research Abstract

The function of oncogenic Nup98-fusion protein remains almost unknown. Our study demonstrated that Nup98-Hox localizes as small dots in the nucleus that frequently associated with specific histone modifications. Furthermore, the formation of these nuclear dots was dependent on the FG repeats of Nup98 and its associated factors.

Research Products

• [Journal Article] The role of Importin-βs in the maintenance and lineage commitment of mouse embryonic stem cells (2014)
  • Author(s): Sangel P, Oka M, Yoneda Y.
  • Journal Title: FEBS Open Bio. Volume: 4 Issue: 1 Pages: 112-120
  • DOI: 10.1016/j.fob.2014.01.001
  • Peer Reviewed
• [Journal Article] Quantitative regulation of nuclear pore complex components by O-GlcNAcylation (2013)
  • Author(s): Mizuguchi-Hata C, Ogawa Y, Oka M and Yoneda Y.
  • Journal Title: Biochim. Biophys. Acta-Molecular Cell Research Volume: 1833 Issue: 12 Pages: 2682-2689
  • DOI: 10.1016/j.bbamcr.2013.06.008
  • Peer Reviewed
• [Journal Article] Cell Density-dependent Nuclear Accumulation of ELK3 is Involved in Suppression of PAI-1 Expression (2013)
  • Author(s): Tanaka S, Nakano K, Sekimoto T, Oka M and Yoneda Y.
  • Journal Title: Cell Structure and Function Volume: 38 Issue: 2 Pages: 145-154
  • DOI: 10.1247/csf.13007
  • NAID: 130004053896
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Journal Article] Differential role for transcription factor Oct4 nucleocytoplasmic dynamics in somatic cell reprogramming and self-renewal of embryonic stem cells (2013)
  • Author(s): Oka, M., Moriyama, T., Asally, M., Kawakami, K., and Yoneda, Y.
  • Journal Title: The Journal of Biological Chemistry Volume: 288 Issue: 21 Pages: 15085-15097
  • DOI: 10.1074/jbc.m112.448837
  • Peer Reviewed
• [Journal Article] The interaction between importin-alpha and Nup153 promotes importin-alpha/beta-mediated nuclear import. (2012)
  • Author(s): Ogawa, Y., et al.
  • Journal Title: Traffic Volume: 13 Issue: 7 Pages: 934-946
  • DOI: 10.1111/j.1600-0854.2012.01367.x
  • Peer Reviewed
• [Journal Article] The role of Rael in Nup98-fusion mediated leukemogenesis (2011)
  • Author(s): Oka, M., Yoneda, Y.
  • Journal Title: Cell Cycle. Volume: 10 Issue: 12 Pages: 12-12
  • DOI: 10.4161/cc.10.12.15759
  • Peer Reviewed
• [Presentation] エストロゲンレセプターの核-細胞質間移動機構とその生理的意義の解明 (2013)
  • Author(s): 盛山哲嗣, 岡正啓, 米田悦啓
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜, 横浜
  • Year and Date: 2013-09-11
• [Presentation] Importinαによるヒストン結合タンパクRBBP4の核内移行 (2013)
  • Author(s): 辻井聡, 盛山哲嗣, 岡正啓, 米田悦啓
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜, 横浜
  • Year and Date: 2013-09-11
• [Presentation] Nup98融合遺伝子による細胞がん化のメカニズム (2013)
  • Author(s): 岡正啓, 米田悦啓
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜, 横浜
  • Year and Date: 2013-09-11
• [Presentation] 様々なNup98融合タンパク質の細胞内局在・機能の解析 (2013)
  • Author(s): 村苑子,岡正啓,米田悦啓
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Nup98融合遺伝子による細胞がん化のメカニズム (2013)
  • Author(s): 岡正啓, 米田悦啓
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Estrogen receptorの核-細胞質間移動機構とその生理的意義の解明 (2013)
  • Author(s): 盛山哲嗣,岡正啓,米田悦啓
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Importinαによるヒストン結合タンパクRBBP4の核内移行と細胞老化 (2013)
  • Author(s): 辻井聡,盛山哲嗣,岡正啓,米田悦啓
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Comparative Expression/Functional Analysis of Karyopherin-βs in Mouse Embryonic Stem Cells and Differentiated Cells (2013)
  • Author(s): Sangel P, Oka M, Yoneda Y.
  • Organizer: International Conference on Life Science & Biological Engineering
  • Place of Presentation: Tokyo, Japan
• [Presentation] Importinαによるヒストン結合タンパクRBBP4の核内移行 (2013)
  • Author(s): 辻井聡, 盛山哲嗣, 岡正啓, 米田悦啓
  • Organizer: 第65回日本細胞生物学会
  • Place of Presentation: ウインクあいち, 愛知
• [Presentation] Importin αによるヒストン結合タンパクRBBP4の核内移行 (2013)
  • Author(s): 辻井 聡, 盛山 哲嗣, 岡 正啓, 米田 悦啓
  • Organizer: 第６５回日本細胞生物学会
  • Place of Presentation: 愛知
• [Presentation] Estrogen receptor の核－細胞質間移動機構とその生理的意義の解明 (2013)
  • Author(s): 盛山 哲嗣，岡 正啓，米田 悦啓
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Nucleocytoplasmic shuttling of estrogen receptorα (2012)
  • Author(s): Moriyama T, Oka M, Yoneda Y.
  • Organizer: 第64回日本細胞生物学会
  • Place of Presentation: 神戸国際会議場, 神戸
• [Presentation] Differential role for Oct4 nucleocytoplasmic dynamics in somatic cell reprogramming and self-renewal of embryonic stem cells (2012)
  • Author(s): Oka M, Moriyama T, Asally M, Kawakami K, Yoneda Y.
  • Organizer: 第64回日本細胞生物学会
  • Place of Presentation: 神戸国際会議場, 神戸
• [Presentation] Estrogen receptorαSubcellular Distribution and Functions (2012)
  • Author(s): Moriyama T, Oka M, Yoneda Y.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Differential role for Oct4 nucleocytoplasmic dynamics in somatic cell reprogramming and self-renewal of embryonic stem cells. (2012)
  • Author(s): Oka M, 他
  • Organizer: 第６４回日本細胞生物学会
  • Place of Presentation: 神戸
• [Presentation] エストロゲンレセプターの核－細胞質間移動機構とその生理的意義の解明
  • Author(s): 盛山 哲嗣, 岡 正啓, 米田 悦啓
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] Importinαによるヒストン結合タンパクRBBP4の核内移行
  • Author(s): 辻井 聡, 盛山 哲嗣, 岡 正啓, 米田 悦啓
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] Nup98融合遺伝子による細胞がん化のメカニズム
  • Author(s): 岡 正啓, 米田 悦啓
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
  • Invited