Analysis of mitochondrial morphology and movement
Project/Area Number |
23570232
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cell biology
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Research Institution | Rikkyo University (2012-2013) Kyushu University (2011) |
Principal Investigator |
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | ミトコンドリア / オルガネラ |
Research Abstract |
An RNA interference screen using nematode led us to identification of a novel kinasin KLP6 involved in mitochondrial morphology and movement. The dominant-negative KLP6 induced abnormal mitochondrial morphology in HeLa cells. Furthermore, anterograde transport of mitochondria in the neurites of differentiated neuro2a cells was significantly reduced when the dominant negative form was overexpressed, indicating that KLP6 regulates intracellular transport of mitochondria. Downregulation of an inner membrane protein LETM1 induces mitochondrial swelling and fragmentation. Moreover, cristae structure clearly disappeared in the LETM1-knockdown cells. To investigate the LETM1 functions, we attempted to in vitro reconstitute membrane invagination using LETM1 recombinant protein and artificial liposome. This is a good tool to analyze the roles of LETM1 on the formation of mitochondrial inner membrane structures.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] KLP6, a newly identified kinesin that regulates morphology and transport of mitochondria in neuronal cells.2011
Author(s)
Tanaka, K., Sugiura, Y., Ichishita, R., Mihara, K., and Oka, T
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Journal Title
Journal of Cell Science
Volume: 124
Pages: 2457-2465
Related Report
Peer Reviewed
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