Role of VCIP135 in Golgi reorganization

• Project/Area Number: 23570234
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Kyushu University
• Principal Investigator: TOTSUKAWA Go 九州大学, 医学(系)研究科(研究院), 助教 (90399684)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: ゴルジ体 / VCIP135 / ユビキチン化 / オルガネラ / WAC

Research Abstract

A novel VCIP135 binding protein is identified as WAC. WAC directly binds to VCIP135, and activates deubiquitinase activity of VCIP135 in vitro. WAC localizes to the Golgi, and functions as an essential factor for Golgi biogenesis. Interestingly, in Golgi reassembly, WAC functions only in p97/p47 membrane fusion pathway, but not in p97/p37 membrane fusion pathway. On the other hand, WAC has no function in ER biogenesis at all. It is suggested that ubiquitin signaling mediated by VCIP135/WAC plays a specific role in Golgi biogenesis, especially in mitosis.

Research Products

• [Journal Article] Mitotic phosphorylation of VCIP135 blocks p97ATPase-mediated Golgi membrane fusion. (2013)
  • Author(s): Totsukawa, G., Matsuo, A., Kubota, A., Taguchi, Y., and Kondo, H.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 433(2) Issue: 2 Pages: 237-42
  • DOI: 10.1016/j.bbrc.2013.02.090
  • Peer Reviewed
• [Journal Article] VCIP135 deubiquitinase and its binding proteinon (2011)
  • Author(s): Totsukawa G, Kaneko Y, Uchiyama K, TohH, Kondo H
  • Journal Title: WAC Volume: 30(17) Issue: 17 Pages: 3581-3593
  • DOI: 10.1038/emboj.2011.260
  • Peer Reviewed
• [Presentation] ATPase膜融合機構によるゴルジ体形成の新規必須因子 (2013)
  • Author(s): 十津川剛、金子弥生、近藤久雄
  • Organizer: 第36回日本分子生物学会年会、ワークショップ「細胞内機能場におけるプレイヤーの解析から見えてくる機能的ミッシングリンク」
  • Place of Presentation: 神戸市
• [Presentation] VCIP135 deubiquitinase and its binding protein, WAC, in p97 ATPase-mediated membrane fusion (2011)
  • Author(s): Go Totsukawa, Yayoi Kaneko, Kaori Tamura, Hisao Kondo.
  • Organizer: The 10th Global COE International Symposium"Biochemistry and Cell Biology"
  • Place of Presentation: National University of Singapore, Singapore
• [Presentation] p97 ATPase 膜融合機構によるゴルジ体形成の新規必須因子
  • Author(s): 十津川剛、金子弥生、近藤久雄
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Invited