| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Previously we demonstrated that TCF3 phosphorylation by Wnt/HIPK2 signaling is essential for embryonic axis specification. However, there had been still a missing link between Wnt ligands and HIPK2, which may be able to explain how Wnt stimulation trigger HIPK2-mediated phosphorylation of TCF3. In this study, we reported that GSK3 inhibition triggers the phosphorylation of TCF3, a repressor type of TCF proteins and that phosphorylated TCF3 is replaced on the target promoter with a positively acting TCF1 upon Wnt/HIPK2-mediated phosphorylation. Moreover, we reviewed recent findings referring a role of Wnt signaling during vertebrate axis specification in a journal and a book. We are currently working on regulation of HIPK2 by GSK3 and a mutual interaction between HIPK2/TCF3 complex and a novel HIPK2-interacting protein identified by expression screen.
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