The regulation of MUC5AC mucin production by extra cellular matrix

• Project/Area Number: 23570238
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Akita Prefectural University
• Principal Investigator: IWASHITA Jun 秋田県立大学, 生物資源科学部, 助教 (70315597)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: mucin / MUC5AC / collagen / laminin / integrin / 喘息 / ムチン / コラーゲン / 細胞外基質 / インテグリン / Akt / 粘液ムチン

Research Abstract

MUC5AC mucin overproduction is a key feature of asthma as contributes to airway obstruction. We found the production of MUC5AC is regulated in part by signals from extracellular matrix via integrin pathways. We investigated the role of Akt, a typical signal transducer in the integrin pathway, in the regulation of MUC5AC production. When NCI-H292 human airway epithelial cells were cultured on laminin or Matrigel, we found that the activity of Akt was suppressed, as compared to control cells with upregulated MUC5AC production. In contrast, Akt was activated in cells cultured on type IV collagen with downregulated MUC5AC production. These results suggest that a signal from laminin or Matrigel induces upregulation of MUC5AC by suppressing Akt activity, whereas a signal from type IV collagen induces downregulation of MUC5AC, mediated by Akt activation.

Research Products

• [Journal Article] Histone deacetylase induces accelerated maturation in Xenopus laevis oocytes (2013)
  • Author(s): Iwashita, J., Kodama, A., Konno, Y., Abe,(他1名)
  • Journal Title: Dev Growth Differ Volume: 55(3) Pages: 319-329
  • NAID: 40019735015
  • Peer Reviewed
• [Journal Article] Intact structure of EGAM1 homeoproteins and basic amino acid residues in the common homeodomain of EGAM1 and EGAM1C contribute to their nuclear localization in mouse embryonic stem cells (2013)
  • Author(s): Sato, S., Morita, S., Iha, M., Mori, Y., Sugawara, S., Kasuga, K., Kojima, I., Ozaki, N., Muraguchi, H., Okano, K., Iwashita, J., Murata, J., Hosaka, M., Kobayashi, M.
  • Journal Title: J Biosci Bioeng Volume: 1389-1723(13) Pages: 49-2
  • NAID: 110009657192
  • Peer Reviewed
• [Journal Article] Differentiation- and apoptosis-inducing activities of rice bran 5 extracts in a human colon cancer cell line (2013)
  • Author(s): Takashima, A., Ohtomo, M., Kikuchi, T., Iwashita, J., Abe, T ., Hata, K.
  • Journal Title: J Food Sci Technol Volume: 50(3) Pages: 595-599
  • Peer Reviewed
• [Journal Article] Regulation of MUC5AC mucin production by the cell attachment dependent pathway involving integrinβ1 in NCI-H292 human lung epithelial cells (2013)
  • Author(s): Iwashita J., Hongo, K., Ito, Y., Abe, T.(他1名)
  • Journal Title: Advances in Biological Chemistry Volume: 3(1) Pages: 1-10
  • Peer Reviewed
• [Journal Article] Intact structure of EGAM1 homeoproteins and basic amino acid residues in the common homeodomain of EGAM1 and EGAM1C contribute to their nuclear localization in mouse embryonic stem cells (2013)
  • Author(s): Sato, S., Morita, S., Iha, M., Mori, Y., Sugawara, S., Kasuga, K., Kojima, I., Ozaki, N., Muraguchi, H., Okano, K., Iwashita, J., Murata, J., Hosaka, M., Kobayashi, M
  • Journal Title: J Biosci Bioeng Volume: 13 Pages: 49-51
  • NAID: 110009657192
  • Peer Reviewed
• [Journal Article] Regulation of MUC5AC mucin production by the cell attachment dependent pathway involving integrin β1 in NCI-H292 human lung epithelial cells (2013)
  • Author(s): Iwashita J., Hongo, K., Ito, Y., Abe, T., Murata, J
  • Journal Title: Advances in Biological Chemistry Volume: 3(1) Pages: 1-10
  • Peer Reviewed
• [Journal Article] Regulation of MUC5AC mucin production by the cell attachment dependent pathway involving integrin 1 in NCI-H292 human lung epithelial cells. (2013)
  • Author(s): Iwashita J., Hongo, K., Ito, Y., Abe, T and Murata, J
  • Journal Title: Advances in Biological Chemistry Volume: 3 Issue: 01 Pages: 1-10
  • DOI: 10.4236/abc.2013.31001
  • Peer Reviewed
• [Journal Article] Intact structure of EGAM1 homeoproteins and basic amino acid residues in the common homeodomain of EGAM1 and EGAM1C contribute to their nuclear localization in mouse embryonic stem cells. (2013)
  • Author(s): Sato, S., Morita, S., Iha, M., Mori, Y., Sugawara, S., Kasuga, K., Kojima, I., Ozaki, N., Muraguchi, H., Okano, K., Iwashita, J., Murata, J., Hosaka, M., Kobayashi, M.
  • Journal Title: J Biosci Bioeng Volume: S1389-1723 Issue: 2 Pages: 49-2
  • DOI: 10.1016/j.jbiosc.2013.02.007
  • NAID: 110009657192
  • Peer Reviewed
• [Journal Article] Histone deacetylase induces accelerated maturation in Xenopus laevis oocytes. (2013)
  • Author(s): Iwashita, J., Kodama, A., Konno, Y., Abe, T., Murata, J.
  • Journal Title: Dev Growth Differ Volume: 55 Issue: 3 Pages: 319-329
  • DOI: 10.1111/dgd.12038
  • NAID: 40019735015
  • Peer Reviewed
• [Journal Article] Effects of ectopic expression of EGAM1 homeoproteins and an HDAC inhibitor sodium butyrate on the induction of endogenous gene expression in mouse fibroblast NIH3T3 cells (2012)
  • Author(s): Mori Y., Suzuki A., Sato Sho, Sugawara S., Kasuga K., Kojima I., Iwashita J. and Kobayashi M Mori, Y., Iwashita, J and Kobayashi, M.(他5名)
  • Journal Title: Tohoku Journal of Animal Science and Technology Volume: 62 Pages: 8-14
  • Peer Reviewed
• [Journal Article] Generative cell-specific activation of the histone H2A gene promoter of Lilium longiflorum in tobacco (2012)
  • Author(s): UEDA, K., Ono, M., Iwashita, J., Wabiko, H., Inoue, M.
  • Journal Title: Sexual Plant Reproduction Volume: 25(4) Pages: 247-255
  • Peer Reviewed
• [Journal Article] The histone deacetylase inhibitor trichostatin A induces cell cycle arrest and rapid upregulation of gadd45βin LS174T human colon cancer cells (2012)
  • Author(s): Taniguchi, T., Iwashita, J., Murata, J, Ueda, K. (他1名)
  • Journal Title: Advances in Biological Chemistry Volume: 2 Pages: 43-50
• [Journal Article] Effects of ectopic expression of EGAM1 homeoproteins and an HDAC inhibitor sodium butyrate on the induction of endogenous gene expression in mouse fibroblast NIH3T3 cells. (2012)
  • Author(s): Mori, Y., Suzuki, A., Sato, S., Sugawara S., Kasuga K., Kojima, I., Iwashita, J and Kobayashi, M.
  • Journal Title: Tohoku Journal of Animal Science and Technology Volume: 62 Pages: 8-14
  • Peer Reviewed
• [Journal Article] Generative cell-specific activation of the histone gH2A gene promoter of Lilium longiflorum in tobacco. (2012)
  • Author(s): Ueda K., Ono M., Iwashita J., Wabiko H., Inoue M
  • Journal Title: Sexual Plant Reproduction Volume: 25 Issue: 4 Pages: 247-255
  • DOI: 10.1007/s00497-012-0194-3
  • Peer Reviewed
• [Journal Article] The histone deacetylase inhibitor trichostatin A induces cell cycle arrest and rapid upregulation of gadd45b in LS174T human colon cancer cells (2012)
  • Author(s): Tomoyuki Taniguchi, Jun Iwashita, Jun Murata, Kenji Ueda and Tatsuya Abe
  • Journal Title: Advances in Biological Chemistry Volume: 2 Issue: 01 Pages: 43-50
  • DOI: 10.4236/abc.2012.21005
  • Peer Reviewed
• [Journal Article] Inhibition of E-cadherin dependent cell-cell contact promotes MUC5AC mucin production through activation of epidermal growth factor receptors (2011)
  • Author(s): Iwashita, J., Ose, K., Ito, H., Murata, J.(他1名)
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75(4)
  • NAID: 10028271470
• [Journal Article] Differentiation- and apoptosis-inducing activities of rice bran 5 extracts in a human colon cancer cell line. (2011)
  • Author(s): Akiko Takashima, Masanobu Ohtomo, Tsugio Kikuchi, Jun Iwashita ,Tatsuya Abe, Keishi Hata
  • Journal Title: J Food Sci Technol Volume: 1 Issue: 3 Pages: 1-5
  • DOI: 10.1007/s13197-011-0368-2
  • Peer Reviewed
• [Journal Article] Akt induces down-regulation of MUC5AC production in NCI-H292 human airway epithelial cells cultured on extracellular matrix
  • Author(s): Iwashita, J., Ito, Y, Yokoo, M., Takahashi,(他1名)
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: (印刷中)
  • Peer Reviewed
• [Presentation] Type IV collagen down-regulates MUC5AC production through integrinβ1/phospho-Akt pathway in NCI-H292 human lung epithelial cells (2013)
  • Author(s): 伊藤佑歩、岩下淳、工藤志帆、木村大地、村田純
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2013-12-03
• [Presentation] インテグリンを介した細胞外基質からのシグナルによるMUC5ACムチン産生の制御 (2013)
  • Author(s): 伊藤佑歩、岩下淳、工藤志帆、横尾万里、村田純
  • Organizer: 第45回日本結合組織学会
  • Place of Presentation: 和歌山市
  • Year and Date: 2013-06-28
• [Presentation] IV 型コラーゲンからのシグナルはインテグリンβ1 / Akt 経路を介してMUC5AC 産生を抑制する (2013)
  • Author(s): 伊藤佑歩、岩下淳、工藤志保、木村大地、村田純
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸市
• [Presentation] インテグリンを介した細胞外基質からのシグナルによるMUC5ACムチン産生の制御 (2013)
  • Author(s): 伊藤佑歩、岩下淳、工藤志帆、横尾万里、村田純
  • Organizer: 日本結合組織学会
  • Place of Presentation: 和歌山市
• [Presentation] Regulation of MUC5AC mucin production by cell adhesion-dependent pathway involving integrin beta1 (2012)
  • Author(s): 伊藤佑歩、横尾万里、工藤志帆、岩下淳
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 横浜市
• [Presentation] インテグリン経路によるムチン産生の制御 (2012)
  • Author(s): 高橋直、宮田阿依、岩下淳
  • Organizer: 第78回日本生化学会東北支部会例会・シンポジウム
  • Place of Presentation: 山形市
• [Presentation] Regulation of MUC5AC mucin production by cell adhesion-dependent pathway involving integrin beta1 (2012)
  • Author(s): Yuuho Ito, Mari Yokoo, Shiho Kudo, Jun Iwashita
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡市 福岡国際会議場
• [Presentation] Inhibition of Akt kinase induces overproduction of MUC5AC mucin in NCI-H292 human lung cancer cells (2011)
  • Author(s): 宮田阿依、岩下淳
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 福岡市
• [Presentation] ヒト肺癌細胞株NCI-H292におけるAktキナーゼを介したMUC5ACムチンの産生制御 (2011)
  • Author(s): 宮田阿依、岩下淳
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜