Roles of COPII-mediated vesicular transport in higher animals
| Project/Area Number |
23570239
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
TANI KATSUKO 東京薬科大学, 生命科学部, 教授 (40266896)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 小胞体 / ペルオキシソーム / p125 / Sec16B / 脂肪滴 / 小胞輸送 / 精子形成 |
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| Research Abstract |
Coat protein complex II (COPII)-coated vesicles mediate protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus. In this study, we analyzed functions of two COPII-interacting proteins, p125 and Sec16B. We found that loss of p125 reduced the interaction between Sec23 (an inner layer component of COPII) and Sec31 (an outer layer component of COPII), suggesting a role in the assembly of COPII. We also demonstrated that Sec16B is involved in the transport of Pex16, a peroxisomal membrane protein, from the ER to peroxisomes. Our results with mutant Sec16B proteins suggest that ER exit sites, which are an ER subdomain where COPII vesicles are formed, are not involved in this process. In addition, we found that Sec16B might be involved in lipid droplet biogenesis in HeLa cells.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Phosphatidic acid-preferring phospholipase A1 regulates mitochondrial dynamics.2014
Author(s)
Baba T, Kashiwagi Y, Arimitsu N, Kogure T, Edo A, Maruyama T, Nakao K, Nakanishi H, Kinoshita M, Frohman MA, Yamamoto A, Tani K.
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Journal Title
Journal of Biological Chemistry
Volume: 289
Issue: 16
Pages: 11497-11511
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Roles of SAM and DDHD domains in mammalian intracellular phospholipase A_1 KIAA0725p2012
Author(s)
Inoue, H., Baba, T., Sato, S., Ohtsuki, R., Takemori, A., Watanabe, T., Tagaya, M., and Tani, K.
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Journal Title
Biochim Biophys Acta
Volume: 1823
Pages: 930-939
Related Report
Peer Reviewed
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[Journal Article] Roles of SAM and DDHD domains in mammalian intracellular phospholipase A1 KIAA0725p2012
Author(s)
Inoue, H., Baba, T., Sato, S., Ohtsuki, R., Takemori, A., Watanabe, T., Tagaya, M., and Tani, K.
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Journal Title
Biochim. Biophys. Acta
Volume: 1823 (*, These authors contributed equally to this work.)
Issue: 4
Pages: 930-939
DOI
Related Report
Peer Reviewed
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[Presentation] Sec16B is involved in the ER export of the peroxisomal membrane biogenesis factor Pex16 in mammalian cells2011
Author(s)
Yonekawa, S., Furuno, A., Baba, T., Fujiki, Y., Tagaya, M., and Tani, K.
Organizer
第63回日本細胞生物学会大会
Place of Presentation
北海道
Related Report
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[Presentation] Sec16B is involved in the ER export of the peroxisomal membrane proteins Pex3 and Pex16 in mammalian cells2011
Author(s)
Yonekawa, S., Furuno, A., Baba, T., Fujiki, Y., Ogasawara, Y., Yamamoto, A., Tagaya, M., and Tani, K.
Organizer
Annual Meeting of the American Society for Cell Biology
Place of Presentation
Denver, CO, USA
Related Report
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