| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Coat protein complex II (COPII)-coated vesicles mediate protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus. In this study, we analyzed functions of two COPII-interacting proteins, p125 and Sec16B. We found that loss of p125 reduced the interaction between Sec23 (an inner layer component of COPII) and Sec31 (an outer layer component of COPII), suggesting a role in the assembly of COPII. We also demonstrated that Sec16B is involved in the transport of Pex16, a peroxisomal membrane protein, from the ER to peroxisomes. Our results with mutant Sec16B proteins suggest that ER exit sites, which are an ER subdomain where COPII vesicles are formed, are not involved in this process. In addition, we found that Sec16B might be involved in lipid droplet biogenesis in HeLa cells.
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