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Study of induction mechanism of autophagy using autophagy induction system

Research Project

Project/Area Number 23570241
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

KAMADA Yoshiaki  基礎生物学研究所, 多様性生物学研究室, 助教 (20291891)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords栄養環境 / シグナル伝達 / 出芽酵母 / 生化学 / 細胞生物学 / ラパマイシン / TOR / オートファジー / TOR
Research Abstract

Nutrients are indispensable for life. Thus, perception of the nutrient environment is also essential for cells. To recognize cellular nutrient conditions, eukaryotic cells employ Tor (target of rapamycin) protein kinase. However, it is not clear the mechanism how Tor perceives nutrient signals.
Our research group established a method to monitor Tor activity in vivo. The aim of our research group is to reveal the molecular mechanisms of how Tor receives nutrient signals.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (19 results)

All 2014 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (13 results) (of which Invited: 1 results) Remarks (2 results)

  • [Journal Article] Amino acid residues required for Gtr1p-Gtr2p complex formation and its interactions with the Ego1p-Ego3p complex and TORC1 components in yeast2014

    • Author(s)
      Takeshi Sekiguchi, Yoshiaki Kamada, Nobuaki Furuno, Minoru Funakoshi and Hideki Kobayashi
    • Journal Title

      Genes to Cells

      Volume: 19 Issue: 6 Pages: 1-15

    • DOI

      10.1111/gtc.12145

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The role of autophagy in genome stability through suppression of abnormal mitosis under starvation.2013

    • Author(s)
      Aiko Matsui, Yoshiaki Kamada, Akira Matsuura
    • Journal Title

      PLOS Genetics

      Volume: 9 Issue: 1 Pages: 1003245-1003245

    • DOI

      10.1371/journal.pgen.1003245

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of autophagy in genome stability through suppression of abnormal mitosis under starvation2013

    • Author(s)
      Matsui, A., Kamada, Y., Matsuura, A.
    • Journal Title

      PLOS genetics

      Volume: 9

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Guideline for the use and interpretation of assays for monitoring autophagy2012

    • Author(s)
      Klionsky DJ,et al
    • Journal Title

      Autophagy

      Volume: 8 Issue: 4 Pages: 445-544

    • DOI

      10.4161/auto.19496

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 栄養センサーTor複合体1はどのようにして栄養環境を把握するか?2013

    • Author(s)
      鎌田芳彰
    • Organizer
      第36回日本分子生物学会年会ワークショップ
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] トア複合体1の栄養感知メカニズム解明の挑戦2013

    • Author(s)
      鎌田芳彰
    • Organizer
      第3回TOR研究会
    • Place of Presentation
      基礎生物学研究所
    • Year and Date
      2013-10-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 窒素源飢餓条件下でのオートファジーによる細胞周期進行の二段階制御機構2013

    • Author(s)
      松井愛子, 鎌田芳彰, 松浦彰
    • Organizer
      酵母遺伝フォーラム第46回研究報告会
    • Place of Presentation
      東北学院大学
    • Year and Date
      2013-09-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 栄養センサーTor複合体はどのようにして栄養環境を把握するか?2013

    • Author(s)
      鎌田芳彰
    • Organizer
      第2回TOR研究会
    • Place of Presentation
      神戸大学
    • Year and Date
      2013-03-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 窒素源飢餓条件下でのオートファジーによる細胞周期進行の二段階制御機構2013

    • Author(s)
      松井愛子、鎌田芳彰、松浦彰
    • Organizer
      酵母遺伝フォーラム第46回研究報告会
    • Place of Presentation
      東北学院大学(宮城県仙台市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 栄養飢餓条件下でのオートファジー依存的な細胞周期進行におけるTORC1の役割2012

    • Author(s)
      松井愛子, 鎌田芳彰, 松浦彰
    • Organizer
      第35回日本分子生物学会年会ワークショップ
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-12-11
    • Related Report
      2013 Final Research Report
  • [Presentation] オートファジー依存的な細胞周期進行機構とそのゲノム安定性への関与2012

    • Author(s)
      松井愛子, 鎌田芳彰, 松浦彰
    • Organizer
      酵母遺伝フォーラム第45回研究報告会
    • Place of Presentation
      京都大学
    • Year and Date
      2012-09-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Monitoring of Tor complex1 activity by immunoblot of Atg132011

    • Author(s)
      Kamada Y.
    • Organizer
      第34回日本分子生物学会年会ワークショップ
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 栄養飢餓条件下での細胞周期進行におけるオートファジーの役割2011

    • Author(s)
      松井愛子, 鎌田芳彰, 松浦彰
    • Organizer
      酵母遺伝フォーラム第44回研究報告会
    • Place of Presentation
      九州大学
    • Year and Date
      2011-09-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Yeast Tor complex1 regulates autophagy by direct phosphorylation of Atg13 Yeast Cell Biology2011

    • Author(s)
      Kamada Y.
    • Place of Presentation
      Cold Spring Harbor Laboratory (アメリカ)
    • Year and Date
      2011-08-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Monitoring of Tor complex1 activity by immunoblot of Atg132011

    • Author(s)
      鎌田芳彰
    • Organizer
      第34回日本分子生物学会年会ワークショップ(招待講演)
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 栄養センサーTor複合体1はどのようにして栄養環境を把握するか?

    • Author(s)
      鎌田芳彰
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 栄養センサーTor複合体はどのようにして栄養環境を把握するか?

    • Author(s)
      鎌田芳彰
    • Organizer
      第2回TOR研究会
    • Place of Presentation
      神戸大学(兵庫県神戸市)
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.nibb.ac.jp/sections/evolutionary_biology_and_biodiversity/diversity/AssisProf/kamada.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 基礎生物学研究所ホームページ

    • URL

      http://www.nibb.ac.jp/sections/evolutionary_biology_and_biodiversity/diversity/AssisProf/kamada.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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