| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Nuclear import of molecular chaperones HSP70 is up-regulated during cellular thermal stresses. However, the mechanism of nuclear transport and physiological significance of HSP70 in the nucleus remains to be unknown. Using our in vitro transport system under heat stress conditions, we identified a novel nuclear import carrier named Hikeshi that mediates heat-stress-induced nuclear import of HSP70. Unlike importin-beta-family-mediated nuclear transport, the Hikeshi-mediated nuclear import of HSP70 is coupled with the ATPase cycle of HSP70. In human living cells, depletion of Hikeshi reduces cell viability during recovery from heat stress conditions, and delays the attenuation and reversion of the heat stress response significantly. Nuclear HSP70 rescues the effect of Hikeshi-depletion partially, suggesting that HSP70 counteracts to the heat stress damages by acting in the nucleus.
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