Mechanism of the replication stress response of variable replication fork complexes
| Project/Area Number |
23570243
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
YOSHIZAWA=SUGATA Naoko 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (30344071)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ゲノム不安定性 / 多能性幹細胞 / 初期胚 / 複製フォーク / 複製ストレス / ES細胞 / AND-1 / Tipin / Rif1 / クロマチン免疫沈降 / 胎生幹細胞 / Timeless / DNA修復 / 未分化 |
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| Research Abstract |
In attempts to identify fork complex components and its binding proteins, we purified Timeless complexes and found that it specifically contains phosphorylated Mcm4 proteins in the chromatin fraction. We also found that the replication fork proteins AND-1 and Tipin, and a replication timing regulator Rif1, are highly expressed in the undifferentiated mouse embryonic stem (ES) cells. Among them, Rif1 is highly correlated with the undifferentiated states of ES cells. Interestingly, Rif1 may play dual roles in ES cells; In addition to its counteraction with spontaneous differentiation, Rif1 also inhibits the expression of 2 cell-specific genes, which are involved in promoting reprogamming for pluripotency. We found that Rif1 binds to the promoter of differentiation-regulating genes.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Role of multifunctional transcription factor TFII-I and putative tumour suppressor DBC1 in cell cycle and DNA double strand damage repair2013
Author(s)
Tanikawa M, Wada Hiraike O, Yoshizawa Sugata N , Shirane A, Hirano M, Hiraike H, Miyamoto Y, Sone K, Ikeda Y, Kashiyama T, Oda K, Kawana K, Katakura Y, Yano T, Masai H, Roy AL, Osuga Y, Fujii T
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Journal Title
Br. J. Cancer
Volume: 109
Pages: 3042-8
Related Report
Peer Reviewed
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[Journal Article] Role of multifunctional transcription factor TFII-I and putative tumour suppressor DBC1 in cell cycle and DNA double strand damage repair.2013
Author(s)
Tanikawa, M., Wada-Hiraike, O., Yoshizawa-Sugata, N., Shirane A, Hirano M, Hiraike H, Miyamoto, Y., Sone, K., Ikeda, Y., Kashiyama, T., Oda K, Kawana K, Katakura Y, Yano T, Masai, H., Roy AL, Osuga, Y. and Fujii, T.
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Journal Title
Br. J. Cancer.
Volume: 109
Issue: 12
Pages: 3042-3048
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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