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Gemline development regulation by maternally localized factor PEM

Research Project

Project/Area Number 23570252
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Developmental biology
Research InstitutionTohoku University (2013)
Osaka University (2011-2012)

Principal Investigator

KUMANO Gaku  東北大学, 生命科学研究科, 教授 (80372605)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsホヤ / 生殖細胞 / 転写抑制 / 母性局在因子 / PEM / 生殖系列 / 初期発生 / マボヤ
Research Abstract

Suppression of zygotic transcription in early embryonic germline cells is tightly linked to its separation from the somatic lineage. Many invertebrate embryos utilize localized maternal factors that are successively inherited by the germline cells for silencing the germline. Germline quiescence has also been associated with the underphosphorylation of Ser2 of RNA polymerase II. Using an ascidian Halocynthia roretzi, we identified a first deuterostome example of a maternally localized factor, PEM, which represses germline transcription through Ser2 underphosphorylation. Our results suggest that non-homologous proteins, PEM, Pgc of Drosophila, and PIE-1 of C. elegans, repress germline gene expression through analogous functions: by keeping Ser2 underphosphorylated through binding to the P-TEFb complex, a kinase that phosphorylates Ser2. The present study provides an interesting example of evolutionary constraint on how a mechanism of germline silencing can evolve in diverse animals.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (37 results)

All 2014 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (28 results) (of which Invited: 4 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Regulation of the number of cell division rounds by tissue-specific transcription factors and Cdk inhibitor during ascidian embryogenesis.2014

    • Author(s)
      Kuwajima, M., Kumano, G., and Nishida, H.
    • Journal Title

      PLOS ONE

      Volume: 9 Issue: 3 Pages: e90188-e90188

    • DOI

      10.1371/journal.pone.0090188

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcription factor Tbx6 plays a central role in fate determination between mesenchyme and muscle in embryos of the ascidian, Halocynthia roretzi.2014

    • Author(s)
      Kumano, G., Negoro, N., and Nishida, H.
    • Journal Title

      Dev. Growth Differ.

      Volume: 56 Issue: 4 Pages: 310-322

    • DOI

      10.1111/dgd.12133

    • NAID

      40020059339

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Neurula rotation determines left-right asymmetry in ascidian tadpole larvae2012

    • Author(s)
      Nishide, K., Nishida, H.
    • Journal Title

      Development

      Volume: 139 Issue: 8 Pages: 1467-1475

    • DOI

      10.1242/dev.076083

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Polarizing animal cells via mRNA localization in oogenesis and early development2012

    • Author(s)
      Kumano, G.
    • Journal Title

      Dev.Growth and Differ.

      Volume: 54 Issue: 1 Pages: 1-18

    • DOI

      10.1111/j.1440-169x.2011.01301.x

    • NAID

      10030404286

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A maternal factor unique to ascidians silences the germline via binding to P-TEFb and RNAP II regulation2011

    • Author(s)
      Kumano G, Takatori N, Negishi T, Takada T, Nishida H
    • Journal Title

      Current Biology

      Volume: 21 Issue: 15 Pages: 1308-13

    • DOI

      10.1016/j.cub.2011.06.050

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The transcription factor FoxB mediates temporal loss of cellular competence for notochord induction in ascidian embryos2011

    • Author(s)
      Hashimoto, H., Nishida, H.
    • Journal Title

      Development

      Volume: 138 Issue: 14 Pages: 2591-2600

    • DOI

      10.1242/dev.070383

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] マボヤ神経胚における「くびれ」形成機構の解析2014

    • Author(s)
      熊野岳
    • Organizer
      第4回JMBIOフォーラム
    • Place of Presentation
      東京
    • Year and Date
      2014-01-16
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] ホヤ幼生の尾が形づくられる仕組み2013

    • Author(s)
      熊野岳
    • Organizer
      秋の浅虫研究会ミニシンポ
    • Place of Presentation
      浅虫 青森
    • Year and Date
      2013-10-19
    • Related Report
      2013 Final Research Report
  • [Presentation] ホヤの原腸形成2013

    • Author(s)
      熊野岳
    • Organizer
      かたちまつり3
    • Place of Presentation
      高槻 大阪
    • Year and Date
      2013-10-12
    • Related Report
      2013 Final Research Report
  • [Presentation] マボヤ神経胚における「くびれ」形成機構の解析2013

    • Author(s)
      熊野岳
    • Organizer
      第84回日本動物学会「ホヤの生物学談話会」
    • Place of Presentation
      岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] マボヤ初期胚における生殖細胞系列での転写抑制解除機構の解析2013

    • Author(s)
      宮奥香理
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Mechanism of the"KUBIRE"formation in the neurula embryo of the ascidian, Halocynthia roretzi2013

    • Author(s)
      Kumano G
    • Organizer
      46^<th> Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Matsue Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Mechanism of Formation of the"Neurula Small Waist"in the Ascidian, Halocynthia roretzi2013

    • Author(s)
      Kumano G
    • Organizer
      The 23^<rd> CDB Meeting Building Multicellular Systems from Cellular Cross-Talk
    • Place of Presentation
      Kobe Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] マボヤ神経胚における「くびれ」形成機構の解析2013

    • Author(s)
      熊野岳
    • Organizer
      第84回日本動物学会、第33回ホヤの生物学談話会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Mechanism of the “KUBIRE” formation in the neurula embryo of the ascidian, Halocynthia roretzi.2013

    • Author(s)
      Kumano, G., Zenpuku, S. and Nishida, H.
    • Organizer
      46th Annual Meeting for the Japanese Society of Developmental Biologists (Cosponsor APDBN)
    • Place of Presentation
      松江
    • Related Report
      2013 Annual Research Report
  • [Presentation] Mechanism of Formation of the “Neurula Small Waist” in the Ascidian, Halocynthia roretzi.2013

    • Author(s)
      Kumano, G., Zenpuku, S. and Nishida, H.
    • Organizer
      The 23rd CDB Meeting “Building Multicellular Systems from Cellular Cross-Talk”
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] Halocynthia roretzi, a Japanese ascidian species, as a model animal for the study of chordate embryogenesis2012

    • Author(s)
      Kumano G
    • Organizer
      The 2nd Annual Meeting for Whole-Organism Science Society
    • Place of Presentation
      Hyogo Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] ホヤ胚発生における発生運命と細胞分裂の回数制御の関係2012

    • Author(s)
      桑島真美
    • Organizer
      第83回日本動物学会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Not mRNA非対称分配による中胚葉と内胚葉運命の分離機構2012

    • Author(s)
      高鳥直士
    • Organizer
      第83回日本動物学会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] PEM, a localized maternal factor unique to ascidians, represses germline gene expression through RNAP II regulation and binding to p-TEFb2012

    • Author(s)
      Kumano G
    • Organizer
      The 58th and 60th NIBB Conference Germline Specification, Sex, and Stem Cells
    • Place of Presentation
      Okazaki Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] マボヤ神経胚における「くびれ」形成機構の解析2012

    • Author(s)
      熊野岳
    • Organizer
      ホヤ研究会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] Roles for maternally-localized PEM in ascidian germline development2012

    • Author(s)
      Kumano G
    • Organizer
      Joint Meeting of JSDB 45th and JSCB 64th
    • Place of Presentation
      Kobe Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] A localized factor polarizes mesendoderm cells and separates mesoderm and endoderm fates in the ascidian embryo2012

    • Author(s)
      Takatori, N
    • Organizer
      Joint Meeting of JSDB 45th and JSCB 64th
    • Place of Presentation
      Kobe Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Roles for maternally-localized PEM in ascidian germline development.2012

    • Author(s)
      Kumano, G., Negishi, T., Taktori, N. and Nishida, H.
    • Organizer
      Joint Meeting of JSDB 45th and JSCB 64th
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] localized factor polarizes mesendoderm cells and separates mesoderm and endoderm fates in the ascidian embryo.2012

    • Author(s)
      Takatori, N., Kumano, G., Saiga, H. and Nishida, H.
    • Organizer
      Joint Meeting of JSDB 45th and JSCB 64th
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] PEM, a localized maternal factor unique to ascidians, represses germline gene expression through RNAP II regulation and binding to p-TEFb.2012

    • Author(s)
      Kumano, G., Takatori, N., Negishi, T. and Nishida, H.
    • Organizer
      The 58th and 60th NIBB Conference “Germline-Specification, Sex, and Stem Cells-”
    • Place of Presentation
      Okazaki, Japan
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Not mRNA 非対称分配による中胚葉と内胚葉運命の分離機構2012

    • Author(s)
      高鳥直士、熊野岳、西駕秀俊、西田宏記
    • Organizer
      第83回日本動物学会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] Halocynthia roretzi, a Japanese ascidian species, as a model animal for the study of chordate embryogenesis.2012

    • Author(s)
      Kumano, G., Takatori, N., and Nishida, H.
    • Organizer
      The 2nd Annual Meeting for Whole-Organism Science Society
    • Place of Presentation
      Hyogo
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] マボヤ胚における生殖細胞系列での転写抑制機構2011

    • Author(s)
      熊野岳
    • Organizer
      日本発生生物学会秋季シンポジウム
    • Place of Presentation
      岡崎
    • Related Report
      2013 Final Research Report
  • [Presentation] マボヤ胚母性局在因子PEMによる生殖細胞系列での転写抑制機構2011

    • Author(s)
      熊野岳
    • Organizer
      第82回大会日本動物学会
    • Place of Presentation
      旭川
    • Related Report
      2013 Final Research Report
  • [Presentation] PEM, a localized maternal factor unique to ascidians, represses germline gene expression through RNAP II regulation and binding to p-TEFb2011

    • Author(s)
      Kumano G
    • Organizer
      6th International Tunicate Meeting
    • Place of Presentation
      Montreal Canada
    • Related Report
      2013 Final Research Report
  • [Presentation] マボヤ胚における生殖細胞系列での転写抑制機構2011

    • Author(s)
      熊野岳
    • Organizer
      日本発生生物学会秋季シンポジウム
    • Place of Presentation
      岡崎カンファレンスセンター(岡崎)
    • Related Report
      2011 Research-status Report
  • [Presentation] マボヤ胚母性局在因子PEMによる生殖細胞系列での転写抑制機構2011

    • Author(s)
      熊野岳、高鳥直士、根岸剛文、高田樹、西田宏記
    • Organizer
      第82回大会日本動物学会
    • Place of Presentation
      旭川市大雪クリスタルホール(旭川)
    • Related Report
      2011 Research-status Report
  • [Presentation] PEM, a localized maternal factor unique to ascidians, represses germline gene expression through RNAP II regulation and binding to p-TEFb.2011

    • Author(s)
      Kumano, G., Takatori, N., Negishi, T., Takada, T. and Nishida, H.
    • Organizer
      6th International Tunicate Meeting
    • Place of Presentation
      McGill University (Montreal, Canada)
    • Related Report
      2011 Research-status Report
  • [Book] New Principles in Developmental Processes2014

    • Author(s)
      Kumano G
    • Publisher
      Springer
    • Related Report
      2013 Final Research Report
  • [Book] Taxon-specific maternal factors for germline specification. In Kondoh, H. and Kuroiwa, A. (ed) New Principles in Developmental Processes.2014

    • Author(s)
      Kumano, G.
    • Total Pages
      321
    • Publisher
      Springer, Heidelberg.
    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.biology.tohoku.ac.jp/lab-www/asamushi/kumano_lab/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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