| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Research Abstract |
In the amphibian retinal regeneration RPE cells play a critical role that RPE cells transdifferentiate into retinal cells. Immediate after retinal removal, RPE cells detach from the basement membrane and migrate to the retinal vascular membrane, where RPE cells form an epithelial sheet. Using a tissue culture model we have analyzed the expression of Rx and Pax6, essential genes to retinal development, in RPE cells how it is related to the alteration of cell-cell and cell-extracellular matrix interaction. Expression of Rx appears to be up-regulated by the detachment of RPE cells from the basement membrane, while expression of Pax6 was observed in cells without gap junction. Cell proliferation was also observed in the RPE cells that have lost gap junction. The initial detachment of RPE cells from the basement membrane appears to be stimulated by the expression of Matrix metalloproteinase (MMPs), since the suppression of MMPs does not induce transdifferentiation.
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