Alteration in Cell-cell and cell-ECM interaction triggers amphibian retinal regeneration
| Project/Area Number |
23570255
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Developmental biology
|
|---|
| Research Institution | Nara Women's University |
|---|
Principal Investigator |
ARAKI Masasuke 奈良女子大学, 自然科学系, 教授 (00118449)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
|---|
| Keywords | 網膜再生 / ツメガエル / 網膜色素上皮細胞 / 細胞結合 / 細胞間結合 / 色素上皮細胞 / MMP / ギャップ結合 / N-cadherin / 細胞環境 |
|---|
| Research Abstract |
In the amphibian retinal regeneration RPE cells play a critical role that RPE cells transdifferentiate into retinal cells. Immediate after retinal removal, RPE cells detach from the basement membrane and migrate to the retinal vascular membrane, where RPE cells form an epithelial sheet. Using a tissue culture model we have analyzed the expression of Rx and Pax6, essential genes to retinal development, in RPE cells how it is related to the alteration of cell-cell and cell-extracellular matrix interaction. Expression of Rx appears to be up-regulated by the detachment of RPE cells from the basement membrane, while expression of Pax6 was observed in cells without gap junction. Cell proliferation was also observed in the RPE cells that have lost gap junction. The initial detachment of RPE cells from the basement membrane appears to be stimulated by the expression of Matrix metalloproteinase (MMPs), since the suppression of MMPs does not induce transdifferentiation.
|
Report
(4 results)
Research Products
(55 results)
-
-
[Journal Article] Abnormal retinal development associated with FRMD7 mutations2014
Author(s)
Thomas MG, Crosier M, Lindsay S, A Kumar, Araki M, Leroy BP, McLean RJ, V Sheth, Maconachie G, Thomas S, Moore AT, Gottlob I
-
Journal Title
Human Mol Genetics
Volume: (in press)
Related Report
Peer Reviewed
-
-
-
[Journal Article] Abnormal retinal development associated with FRMD7 mutations2014
Author(s)
Thomas MG, M Crosier, S Lindsay, A Kumar, M Araki, B P Leroy, R J McLean, V Sheth, G Maconachie, S Thomas, A T Moore, I Gottlob
-
Journal Title
Human Mol. Genetics
Volume: unknown
Related Report
Peer Reviewed
-
-
-
-
-
-
-
[Journal Article] The clinical and molecular genetic features of idiopathic infantile periodic alternating nystagmus2011
Author(s)
Thomas MG, Crosier M, Lindsay S, Kumar A, Thomas S, Araki M, Talbot CJ, McLean RJ, Surendran M, Taylor K, Leroy BP, Moore AT, Hunter DG, Hertle RW, Tarpey P, Langmann A, Lindner S, Brandner M, Gottlob I
-
Journal Title
Brain
Volume: 134(3)
Pages: 892-902
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
