The regulation of cell movement by the ubiquitin system.

• Project/Area Number: 23570262
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: KINOSHITA Noriyuki 基礎生物学研究所, 形態形成研究部門, 准教授 (30300940)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ユビキチン / 細胞接着分子 / 原腸形成 / 細胞接着 / アフリカツメガエル

Research Abstract

Paraxial protocadherin (PAPC) has been shown to be involved in gastrulation cell movements during early embryogenesis. It is first expressed in the dorsal marginal zone at the early gastrula stage and subsequently restricted to the paraxial mesoderm in Xenopus and zebrafish. Using Xenopus embryos, we found that PAPC is regulated also at the protein level and is degraded and excluded from the plasma membrane in the axial mesoderm by the late gastrula stage. Regulation of PAPC requires poly-ubiquitination that is dependent on phosphorylation in the evolutionarily conserved cytoplasmic domain. We also show that membrane localization of PAPC is associated with low cell motility and its regulation by phosphorylation/ubiquitination is essential for normal Xenopus gastrulation cell movements. Taken together, our findings unveiled a novel mechanism of regulation of a cell adhesion protein and that this system plays a crucial role in vertebrate embryogenesis.

Research Products

• [Journal Article] Transgenic Xenopus laevis for live imaging in cell and developmental biology. (2013)
  • Author(s): Takagi, C., Sakamaki, K., Morita, H., Hara, Y., Suzuki, M., Kinoshita, N. and Ueno, N.
  • Journal Title: Development, Growth & Differentiation Volume: 55 Issue: 4 Pages: 422-433
  • DOI: 10.1111/dgd.12042
  • Peer Reviewed
• [Presentation] Regulation of paraxial protocadherin (PAPC) by a phosphorylation- dependent ubiquitin system is required for Xenopus early development (2013)
  • Author(s): Kai M, and Kinoshita N
  • Organizer: 46th annual meeting for the Japanese society of Developmental Biologists
  • Place of Presentation: 島根県立産業交流会館 くにびきメッセ 島根県・松江市)
• [Presentation] Regulation of paraxial protocadherin (PAPC) by a phosphorylation-dependent ubiquitin system is required for Xenopus early development (2013)
  • Author(s): Kai M and Kinoshita N
  • Organizer: 46th annual meeting for the Japanese society of Developmental Biologists
  • Place of Presentation: Shimane, Japan
  • Invited
• [Presentation] Prickle andβ-TrCP regulate cell adhesion by controlling paraxial protocadherin activity through ubiquitination in Xenopus development (2011)
  • Author(s): Kai M and Kinoshita N
  • Organizer: 44th annual meeting for the Japanese society of Developmental Biologists
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県・宜野湾市)
• [Presentation] Prickle and β-TrCP regulate cell adhesion by controlling paraxial protocadherin activity through ubiquitination in Xenopus development (2011)
  • Author(s): Kai M and KINOSHITA N
  • Organizer: Annual meeting of the Japanaese Society of Developmental Biolodists and Asia pacific Developmental Biology Network
  • Place of Presentation: 沖縄コンベンションセンター（ 沖縄県）