Analysis of relationship between somite segmentation and vertebra segmentation
| Project/Area Number |
23570263
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
TAKAHASHI Yu 国立医薬品食品衛生研究所, 毒性部, 室長 (60321858)
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| Co-Investigator(Renkei-kenkyūsha) |
YASUHIKO Yukuto 国立医薬品食品衛生研究所, 毒性部, 主任 研究官 (40370944)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 体節形成 / 脊椎骨 / resegmentation / 椎間板 / 発生・分化 / マウス / Uncx4.1 / 遺伝学 |
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| Research Abstract |
Formation of the vertebral body from somites involves a process called resegmentation, by which the caudal half of a sclerotome is combined with the rostral half of the next sclerotome. We examined the relationship between resegmentation and rostro-caudal patterning of somite by using the Uncx4.1-LacZ transgene. Our results suggested that in the thoracic and lumbar vertebrae, the Uncx4.1-expressing caudal sclerotome gave rise to the intervertebral disc (IVD) and rostral portion of the vertebral body (VB). In the cervical vertebrae, the caudal sclerotome contributed to the IVD and both ends of the VB. This finding suggests that the rostro-caudal gene expression boundary does not necessarily coincide with the resegmentation boundary. Expression analysis of IVD marker genes including Pax1 in the wild-type, Mesp2 KO, and Ripply1/2 DKO embryos also supported the idea that a metameric pattern of IVD/VB is generated independently of Mesp2/Ripply-mediated rostro-caudal patterning of somite.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Spatiotemporal disorder in endochondral ossification during axial skeleton development in the Mesp2-null mouse: A developmental etiology of spondylocostal dysostosis and spondylothoracic dysostosis.2013
Author(s)
Makino Y, Takahashi Y, Tanabe R, Tamamura Y, Watanabe T, Haraikawa M, Hamagaki M, Hata K, Kanno J, Yoneda T, Saga Y, Goseki-Sone M, Kaneko K, Yamaguchi A, Iimura T
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Journal Title
BONE
Volume: 53
Issue: 1
Pages: 248-258
DOI
Related Report
Peer Reviewed
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