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Analysis of relationship between somite segmentation and vertebra segmentation

Research Project

Project/Area Number 23570263
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Developmental biology
Research InstitutionNational Institute of Health Sciences

Principal Investigator

TAKAHASHI Yu  国立医薬品食品衛生研究所, 毒性部, 室長 (60321858)

Co-Investigator(Renkei-kenkyūsha) YASUHIKO Yukuto  国立医薬品食品衛生研究所, 毒性部, 主任 研究官 (40370944)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords体節形成 / 脊椎骨 / resegmentation / 椎間板 / 発生・分化 / マウス / Uncx4.1 / 遺伝学
Research Abstract

Formation of the vertebral body from somites involves a process called resegmentation, by which the caudal half of a sclerotome is combined with the rostral half of the next sclerotome. We examined the relationship between resegmentation and rostro-caudal patterning of somite by using the Uncx4.1-LacZ transgene. Our results suggested that in the thoracic and lumbar vertebrae, the Uncx4.1-expressing caudal sclerotome gave rise to the intervertebral disc (IVD) and rostral portion of the vertebral body (VB). In the cervical vertebrae, the caudal sclerotome contributed to the IVD and both ends of the VB. This finding suggests that the rostro-caudal gene expression boundary does not necessarily coincide with the resegmentation boundary. Expression analysis of IVD marker genes including Pax1 in the wild-type, Mesp2 KO, and Ripply1/2 DKO embryos also supported the idea that a metameric pattern of IVD/VB is generated independently of Mesp2/Ripply-mediated rostro-caudal patterning of somite.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (6 results)

All 2013 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results)

  • [Journal Article] Metameric pattern of intervertebral disc/vertebral body is generated independently of Mesp2/Ripply-mediated rostro-caudal patterning of somites in the mouse embryo.2013

    • Author(s)
      Takahashi Y, Yasuhiko Y, Takahashi J, Takada S, Johnson RL, Saga Y, Kanno J.
    • Journal Title

      Dev Biol.

      Volume: 380 Issue: 2 Pages: 172-184

    • DOI

      10.1016/j.ydbio.2013.05.020

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spatiotemporal disorder in endochondral ossification during axial skeleton development in the Mesp2-null mouse: A developmental etiology of spondylocostal dysostosis and spondylothoracic dysostosis.2013

    • Author(s)
      Makino Y, Takahashi Y, Tanabe R, Tamamura Y, Watanabe T, Haraikawa M, Hamagaki M, Hata K, Kanno J, Yoneda T, Saga Y, Goseki-Sone M, Kaneko K, Yamaguchi A, Iimura T
    • Journal Title

      BONE

      Volume: 53 Issue: 1 Pages: 248-258

    • DOI

      10.1016/j.bone.2012.11.033

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Metameric pattern of intervertebral disc/vertebral body is generated independently of Mesp2/Ripply-mediated rostro-caudal patterning of somites in the mouse embryo2013

    • Author(s)
      Yu Takahashi, Yukuto Yasuhiko, Jun Takahashi, Shinji Takada, Randy L. Johnson, Yumiko Saga and Jun Kanno
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] Metameric pattern of intervertebral disc/vertebral body is generated independently of Mesp2/Ripply-mediated rostro-caudal patterning of somites in the mouse embryo2013

    • Author(s)
      Yu Takahashi, Yukuto Yasuhiko, Jun Takahashi, Shinji Takada, Randy L. Johnson, Yumiko Saga and Jun Kanno
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] Metameric pattern of vertebral body/intervertebral disc is correlated with Pax1 expression and is formed irrespectively of rostro-caudal patterning of somites2011

    • Author(s)
      高橋雄,安彦行人,相賀裕美子,高田慎治,菅野純
    • Organizer
      第44回日本発生生物学会
    • Place of Presentation
      沖縄
    • Related Report
      2013 Final Research Report
  • [Presentation] Metameric pattern of vertebral body/intervertebral disc is correlated with Pax1 expression and is formed irrespectively of rostro-caudal patterning of somites.2011

    • Author(s)
      高橋 雄, 安彦行人, 相賀裕美子,高田慎治, 菅野 純
    • Organizer
      第44回日本発生生物学会
    • Place of Presentation
      沖縄
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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