• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular network and functional analysis of gonad specific microRNA

Research Project

Project/Area Number 23570265
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Developmental biology
Research InstitutionNational Research Institute for Child Health and Development

Principal Investigator

TAKADA Shuji  独立行政法人国立成育医療研究センター, システム発生・再生医学研究部, 部長 (20382856)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsmicroRNA / セルトリ細胞 / フォリキュラー細胞 / 性分化
Research Abstract

In order to analyze functions of microRNA in gonadogenesis, functions of microRNA-202, which is expressed specifically in gonads, were studied. Since microRNA functions through target mRNA, a target of microRNA-202 was screened by luciferase reporter assay. Also microRNA-202 KO mouse was established by genome editing utilizing TALEN system. The microRNA-202 KO mouse did not show abnormality by macroscopic specification.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (14 results)

All 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (12 results)

  • [Journal Article] Production of Sry knockout mouse using TALEN via oocyte injection.2013

    • Author(s)
      Kato T, Miyata K, Sonobe M, Yamashita S, Tamano M, Miura K, Kanai Y, Miyamoto S, Sakuma T, Yamamoto T, Inui M, Kikusui T, Asahara H, Takada S.
    • Journal Title

      Scientific reports

      Volume: 3 Issue: 1 Pages: 3136-3136

    • DOI

      10.1038/srep03136

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeted gene deletion of miRNAs in mice by TALEN system.2013

    • Author(s)
      Takada S, Sato T, Ito Y, Yamashita S, Kato T, Kawasumi M, Kanai-Azuma M, Igarashi A, Kato T, Tamano M, Amahara H.
    • Journal Title

      PLoS One.

      Volume: 8 Issue: 10 Pages: e76004-e76004

    • DOI

      10.1371/journal.pone.0076004

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] TALEN技術を用いたSry KOマウスの作出2013

    • Author(s)
      加藤朋子,宮田康平,園部未来,山下聡,玉野萌恵,宮本新吾,佐久間哲史,山本卓,乾雅史,菊水健史,浅原弘嗣,高田修治
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫)
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] SOX9はオス生殖腺においてDhhの発現を直接制御する2013

    • Author(s)
      山下聡,加藤朋子,山口勝司,重信秀治,乾雅史,高田修治,浅原弘嗣
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫)
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] ハイスループット・スクリーニング系を用いた卵巣形成関連候補因子の制御因子の探索2013

    • Author(s)
      乾雅史,加藤朋子,五十嵐ありさ,浅原弘嗣,高田修治
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫)
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 生殖腺特異的マイクロRNAの同定と機能解析 Identification and characterisation of gonad-specific microRNA2012

    • Author(s)
      高田修治
    • Organizer
      第35回日本分子生物学会年会 :ワークショップ「哺乳類の性分化・性成熟の新知見:性腺機能を制御する遺伝子ネットワークとその破綻による疾患」
    • Place of Presentation
      福岡国際会議場(福岡)
    • Year and Date
      2012-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] MicroRNA-202 is specifically expressed in the gonads through early to terminal differentiation stages in the mouse2012

    • Author(s)
      Shuji Takada
    • Organizer
      Sixth International Symposium on The Biology of Vertebrate Sex Determination
    • Place of Presentation
      King Kamehameha's Kona Beach Hotel(Hawaii、USA)
    • Year and Date
      2012-04-25
    • Related Report
      2013 Final Research Report
  • [Presentation] microRNA-202は生殖腺特異的に生殖腺の分化初期から発現する2011

    • Author(s)
      高田修治,渡辺義隆,久貝朋子,玉野萌恵,五十嵐ありさ,浅原弘嗣
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2011-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] microRNA-202は生殖腺特異的に生殖腺分化初期から発現する2011

    • Author(s)
      高田修治, 他5名
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] TALEN技術を用いたSry KOマウスの作出

    • Author(s)
      加藤朋子, 宮田康平, 園部未来, 山下聡, 玉野萌恵, 宮本新吾, 佐久間哲史, 山本卓, 乾雅史, 菊水健史, 浅原弘嗣, 高田修治
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] SOX9はオス生殖腺において Dhhの発現を直接制御する

    • Author(s)
      山下聡, 加藤朋子, 山口勝司, 重信秀治, 乾雅史, 高田修治, 浅原 弘嗣
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ハイスループット・スクリーニング系を用いた卵巣形成関連候補因子の制御因子の探索

    • Author(s)
      乾雅史, 加藤朋子, 五十嵐ありさ, 浅原弘嗣, 高田修治
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] MicroRNA-202 is specifically expressed in the gonads through early to terminal differentiation stages in the mouse

    • Author(s)
      Shuji Takada
    • Organizer
      Sixth International Symposium on The Biology of Vertebrate Sex Determination
    • Place of Presentation
      King Kamehameha’s Kona Beach Hotel(Hawaii, USA)
    • Related Report
      2012 Research-status Report
  • [Presentation] 生殖腺特異的マイクロRNAの同定と機能解析(Identification and characterisation of gonad-specific microRNA)

    • Author(s)
      高田 修治
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi