Molecular network and functional analysis of gonad specific microRNA
| Project/Area Number |
23570265
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | National Research Institute for Child Health and Development |
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Principal Investigator |
TAKADA Shuji 独立行政法人国立成育医療研究センター, システム発生・再生医学研究部, 部長 (20382856)
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|---|
| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | microRNA / セルトリ細胞 / フォリキュラー細胞 / 性分化 |
|---|
| Research Abstract |
In order to analyze functions of microRNA in gonadogenesis, functions of microRNA-202, which is expressed specifically in gonads, were studied. Since microRNA functions through target mRNA, a target of microRNA-202 was screened by luciferase reporter assay. Also microRNA-202 KO mouse was established by genome editing utilizing TALEN system. The microRNA-202 KO mouse did not show abnormality by macroscopic specification.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Production of Sry knockout mouse using TALEN via oocyte injection.2013
Author(s)
Kato T, Miyata K, Sonobe M, Yamashita S, Tamano M, Miura K, Kanai Y, Miyamoto S, Sakuma T, Yamamoto T, Inui M, Kikusui T, Asahara H, Takada S.
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Journal Title
Scientific reports
Volume: 3
Issue: 1
Pages: 3136-3136
DOI
Related Report
Peer Reviewed
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[Journal Article] Targeted gene deletion of miRNAs in mice by TALEN system.2013
Author(s)
Takada S, Sato T, Ito Y, Yamashita S, Kato T, Kawasumi M, Kanai-Azuma M, Igarashi A, Kato T, Tamano M, Amahara H.
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Journal Title
PLoS One.
Volume: 8
Issue: 10
Pages: e76004-e76004
DOI
Related Report
Peer Reviewed
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[Presentation] TALEN技術を用いたSry KOマウスの作出2013
Author(s)
加藤朋子,宮田康平,園部未来,山下聡,玉野萌恵,宮本新吾,佐久間哲史,山本卓,乾雅史,菊水健史,浅原弘嗣,高田修治
Organizer
第36回日本分子生物学会年会
Place of Presentation
神戸ポートアイランド(兵庫)
Year and Date
2013-12-05
Related Report
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[Presentation] TALEN技術を用いたSry KOマウスの作出
Author(s)
加藤朋子, 宮田康平, 園部未来, 山下聡, 玉野萌恵, 宮本新吾, 佐久間哲史, 山本卓, 乾雅史, 菊水健史, 浅原弘嗣, 高田修治
Organizer
第36回日本分子生物学会年会
Place of Presentation
神戸ポートアイランド（兵庫県）
Related Report
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