Evolutionary considerations of D-aspartyl endopeptidases and its substrates.
| Project/Area Number |
23570272
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Evolutionary biology
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | D-アミノ酸 / タンパク質分解酵素 / 酸化ストレス / ミトコンドリア / 機能進化 / D-アスパラギン酸 / ラセミ化 / 品質管理 / プロテアーゼ / アフリカツメガエル / ウニ / ハツカダイコン / マイクロトム / 発生 / 受精 |
|---|
| Research Abstract |
D-isomer of Asp (D-Asp) residue is often detected in abnormally aggregated proteins. Therefore it is strongly suggested that formation of D-Asp in proteins is potentially noxious for metabolic turnover. The D-aspartyl endopeptidase (DAEP), which we have discovered from mammals, stereoselectively degrades its substrate at the internal D-Asp residue, and seems to physiologically serve as a kind of quality-control system to maintain the protein homeostasis. On the other hand, a distribution of DAEP in living things is not clear. In African clawed frog (Xenopus laevis), high DAEP activity was detectable in its testes, ovaries and unfertilized eggs, though the frog naturally thrives around 20ºC at the most. In order to elucidate physiological functions of DAEP, we therefore examined comparison of properties of DAEPs, purified from aquatic animals: African clawed frogs and Japanese green sea urchins, with mammalian DAEP.
|
Report
(4 results)
Research Products
(18 results)
-
-
[Journal Article] UV B-irradiation enhances the racemization and isomerizaiton of aspartyl residues and production of N ε-carboxymethyl lysine (CML) in keratin of skin2011
Author(s)
Y.Mori, K.Aki, K.Kuge, N.Yamanaka, S.Tajima, Y.Kaji, R.Nagai, H.Yoshii, N.Yamamoto, N.Fujii, M.Watanabe, T.Kinouchi, N.Fujii
-
Journal Title
J.Chromatogr.B
Volume: 879
Issue: 29
Pages: 3303-3309
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
