Analysis of interaction that facilitated thermo-adaptive evolution of E. coli
| Project/Area Number |
23570277
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOMO Tetsuya 大阪大学, 大学院情報科学研究科, 教授 (00222399)
WATANABE Soichiro 東邦大学, 理学部, 教授 (10287550)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 相互作用 / 実験室進化 / 大腸菌 / 高温適応進化 / 応答解析 / 発現解析 |
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| Research Abstract |
Interaction facilitating growth at high temperature was analyzed by identify of interactor, transcriptome and mutation function analyses. We have identified lactic acid as candidate for major interactor, but we have found that lactic acid production pathway was not activated significantly during thermo-adaptive evolution by transcriptome analyses, and lactate permease gene, lldP, was mutated in late 45 degree adaptation period when interaction diminished.
Six mutations fixed during early 45 degree adaptation were analyzed by estimating contribution to fitness at 45 degree. We found candidate mutations important for interaction appearance and disappearance. These candidate mutations were related to cellular protein stability. This study indicated that interaction facilitating growth at high temperature was achieved by combination of interactor and intracellular protein stability.
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Report
(4 results)
Research Products
(90 results)
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[Presentation] 疑似進化による新規的表現型の獲得
Author(s)
應 ベイウェン, 本田 朋也, 吉田 真理, 岸本 利彦, 津留 三良, 四方 哲也
Organizer
第35回日本分子生物学会年会
Place of Presentation
福岡国際会議場（福岡県）
Related Report
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