| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Research Abstract |
To elucidate the molecular basis of stress-resistance induced by dietary restriction, an in vitro evaluating system for the effects of nutrient-deficiency on the induction of oxidative-stress related genes was established in this study. The expression of metallothionein-Ia (MT-Ia) gene in human hepatoma cells was significantly enhanced by culture in media which lack either fetal calf serum, or cysteine. The treatment with an inhibitor of glycolysis, 2-deoxyglucose, also induced the expression of MT-Ia gene. From these results, it was suggested that human cells have potential to resist against oxidative stress by three different mechanisms, which could be reacquired by dietary restriction.
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