Establishment of highly gene transcriptional regulation mediated by artificial microRNA in soybean
| Project/Area Number |
23580002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Hokkaido University |
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Principal Investigator |
YAMADA Tetsuya 北海道大学, (連合)農学研究科(研究院), 講師 (70374618)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | Glycine max / ダイズ / amiRNA / ジーンサイレンシング / 貯蔵タンパク質 / 機能性成分 / 糖転移酵素 / 形質転換 / サポニン / 形質転換体 / マイクロRNA / miRNA / アグロバクテリウム |
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| Research Abstract |
Artificial mircoRNA (amiRNA) was designed using the precursor sequence of the endogenous soybean miRNA to suppress the expression of target gene efficiently in soybean plants. The constructed amiRNA precursor contains a complementary sequence to the transcript of target gene. Transgenic soybean plants expressing the amiRNA were generated to suppress the gene expression of seed storage protein. The expression of these genes and accumulation of storage protein were significantly decreased in seeds of transgenic soybean. In addition, the function of glycosyltransferase gene for DDMP saponin, which is known to be one of functional chemical compounds in soybean seeds, was characterized by the amiRNA expression system. I demonstrated that the knock-down system established in this study was useful for the functional analysis of genes in soybean plant.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] 2012 年 Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1, which regulates photoperiodic flowering2012
Author(s)
Z. Xia, S. Watanabe, T. Yamada, Y. Tsubokura, H. Nakashima, H. Zhai, T. Anai, S. Sato, T. Yamazaki, S. Lu, H. Wu, S. Tabata and K. Harada
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 109
Issue: 32
Pages: 2155-2164
DOI
Related Report
Peer Reviewed
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