| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
In order to isolate clubroot resistance gene (CR-gene) in cabbage, we mapped 30 markers that can detected polymorphism in CR-gene regions of the susceptible and resistance parents. The recombinant plants, which had crossovers in the CR-gene region, were genotyped using our designed markers to obtain graph genotypes. The inoculation to the graph genotypes revealed that the CR-gene region is located around the KBr marker. We determined the DNA sequence of the CR-gene region from next-generation sequencing analysis of the susceptible parent resistance. The candidate gene region will be sub-cloned from BAC and cosmid clones, already cloned and contained the target region.
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