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Genetic analysis of root traits in Lotus corniculatus produced by gain-of-function strategy

Research Project

Project/Area Number 23580010
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Breeding science
Research InstitutionUniversity of Miyazaki

Principal Investigator

AKASHI Ryo  宮崎大学, 農学部, 教授 (20253809)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsFOXハンティングシステム / セイヨウミヤコグサ / 根の成長と分化 / 根粒形成
Research Abstract

The objective of this study was to investigate useful genes that can increase plant biomass, root elongation and differentiation, and increase rhizobium production rates in legumes. Here, Arabidopsis thaliana cDNAs were randomly transformed into Lotus corniculatus. Using FOX system, a molecular tool to rapidly and efficiently dig useful trait, plants that had overexpressed the introduced genes from Arabidopsis were evaluated. We measured the upper parts and lower parts of the plant, and also the production rates of rhizobia in the roots. From this method, we found plant lines that showed significant differentiations based on the morphological characteristics, including rhizobia production. All information gathered from these results was compiled in a working database. Specially, this database will be prepared and opened for research use. Furthermore, this result is useful for the molecular breeding of glycine to improve glycine production.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (7 results)

All 2014 2013 2012

All Presentation (7 results)

  • [Presentation] Phenotype analysis of the mutant in decreased nodulation of Lotus corniculatus super growing root2014

    • Author(s)
      Tsubasa Yano, Akihiro Yamamoto, Yuichi Saeki, Ryo Akashi
    • Organizer
      植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2013 Final Research Report
  • [Presentation] Phenotype analysis of the mutant in decreased nodulation of Lotus corniculatus super growing root2014

    • Author(s)
      Tsubasa Yano, Akihiro Yamamoto, Yuichi Saeki, Ryo Akashi
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] 根粒着生数が減少したセイヨウミヤコグサスーパールート系統における表現型解析2013

    • Author(s)
      矢野翼、山本昭洋、明石良、佐伯雄一
    • Organizer
      土壌肥料学会年次大会
    • Place of Presentation
      名古屋
    • Year and Date
      2013-09-11
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Phenotype analysis of Lotus corniculatus FOX-SR line reducing nodule number2013

    • Author(s)
      Tsubasa Yano, Akihiro Yamamoto, Yuichi Saeki, Ryo Akashi
    • Organizer
      18th International congress on nitrogen fixation
    • Place of Presentation
      Miyazaki Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Phenotype analysis of Lotus corniculatus FOX-SR line reducing nodule number2013

    • Author(s)
      Tsubasa Yano, Akihiro Yamamoto, Yuichi Saeki, Ryo Akashi
    • Organizer
      18th International Congress on Nitrogen Fixation
    • Place of Presentation
      宮崎
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development and characteristics of FOX-superroots in Lotus corniculatus2012

    • Author(s)
      Ryo Akashi, Yasuyo Himuro, Hidenori Tanaka, Masatsugu Hashiguchi
    • Organizer
      Plant and animal genome XX
    • Place of Presentation
      San Diego, California USA
    • Year and Date
      2012-01-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Development and Characteristics of FOX-superroots In Lotus corniculatus2012

    • Author(s)
      Ryo Akashi, Yasuyo Himuro, Hidenori Tanaka, Masatsugu Hashiguchi
    • Organizer
      Plant and Animal Genome XX
    • Place of Presentation
      Town and Country Hotel, San Diego, CA, USA
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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