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Studies on the genetic mechanism regulating flower opening in rice

Research Project

Project/Area Number 23580012
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Breeding science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

YOSHIDA Hitoshi  独立行政法人農業・食品産業技術総合研究機構, 作物研究所稲研究領域, 主任研究員 (30355565)

Co-Investigator(Kenkyū-buntansha) OHMORI Shinnosuke  独立行政法人農業食品産業技術総合研究機構, 中央農業総合研究センター・作物開発研究領域, 研究員 (50391438)
YATOU Osamu  独立行政法人農業食品産業技術総合研究機構, 中央農業総合研究センター・作物開 発研究領域, 研究領域長 (50355575)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords閉花受粉性 / イネ / 開花 / 花器官 / 鱗被 / 変異体 / 内穎 / 外穎
Research Abstract

Flower opening of rice is achieved by a series of well-organized events, that is, unlocking of lemma and palea, swelling of lodicule, elongation of stamen filaments, anther dehiscence and stigma opening. In this study, we analyzed genetic mechanism that regulates the flower opening of rice using cleistogamous mutants.
From EMS-mutagenized populations, we identified 8 cleistogamous mutants. One of these mutants was a novel allele of the SPW1 gene that specifies lodicule identity. Another mutation, H193mt, is not related to lodicule development, and the causal gene was mapped to a 250kb region on chromosome 1. We also revealed that short-grain mutants with extremely round-shaped grains and small lodicules were cleistogamous. Furthermore, we identified MADS32 as a novel gene involved in development of flower-opening machinery of rice.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (32 results)

All 2014 2013 2012 2011

All Journal Article (7 results) (of which Peer Reviewed: 6 results) Presentation (25 results) (of which Invited: 1 results)

  • [Journal Article] イネ閉花受粉性変異体spw1-clsおよび疎粒変異体M645(lax2-3アレル)識別用PCR-based DNAマーカーの開発2013

    • Author(s)
      田淵宏朗、大森伸之介、西村実、佐藤豊、吉田均
    • Journal Title

      北陸作物学会報

      Volume: 48 Pages: 31-33

    • NAID

      110009985439

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inflorescence Meristem Identity in Rice is Specified by Overlapping Functions of Three AP1/FUL-like MADS Box Genes and PAP2, a SEP MADS Box gene.2012

    • Author(s)
      Kaoru Kobayashi
    • Journal Title

      Plant Cell

      Volume: 24 Issue: 5 Pages: 1848-1859

    • DOI

      10.1105/tpc.112.097105

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Agronomic traits and gene containment capability of cleistogamous rice lines with the <i>superwoman1-cleistogamy</i> mutation2012

    • Author(s)
      Ohmori S., Tabuchi H, Yatou O. and Yoshida H
    • Journal Title

      Breeding Science

      Volume: 62 Issue: 2 Pages: 124-132

    • DOI

      10.1270/jsbbs.62.124

    • NAID

      130004057150

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Is the lodicule a petal Molecular evidence?2012

    • Author(s)
      Yoshida H
    • Journal Title

      Plant Science

      Volume: 184 Pages: 121-128

    • DOI

      10.1016/j.plantsci.2011.12.016

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Analysis and Utilization of a Cleistogamous Mutant of Rice2012

    • Author(s)
      Hitoshi Yoshida
    • Journal Title

      Gamma Field Symposia

      Volume: 49 Pages: 17-25

    • Related Report
      2012 Research-status Report
  • [Journal Article] LAX PANICLE2 of rice encodes a novel nuclear protein and regulates the formation of axillary meristems2011

    • Author(s)
      Hiroaki Tabuchi, Yu Zhang, Susumu Hattori, Minami Omae, Sae Shimizu-Sato, Tetsuo Oikawa, Qian Qian, Minoru Nishimura, Hidemi Kitano, He Xie, Xiaohua Fang, Hitoshi Yoshida, Junko Kyozuka, Fan Chen, and Yutaka Sato
    • Journal Title

      Plant Cell

      Volume: 23 Issue: 9 Pages: 3276-3287

    • DOI

      10.1105/tpc.111.088765

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Flower development in rice2011

    • Author(s)
      Yoshida H. and Nagato Y
    • Journal Title

      Journal of Experimental Botany

      Volume: 62(14) Issue: 14 Pages: 4719-4730

    • DOI

      10.1093/jxb/err272

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 各地で栽培したspw1-cls変異を持つ閉花受粉性イネ系統の開花率と開花/閉花予測地図の試作2014

    • Author(s)
      大森伸之介、大東健太郎、岩崎亘典、佐藤弘一、岡本和之、山崎周一郎、高橋利和、望月篤、藤代淳、野村研、上野直也、細井淳、新井利直、中澤伸夫、矢ケ崎和弘、鈴木亨、佐藤秀人、加藤満、水上優子、吉田朋史、山川智大、松井崇晃、蛯谷武志、小林大樹、笹倉康弘、田野井真、矢頭治、吉田均
    • Organizer
      日本作物学会
    • Place of Presentation
      千葉市
    • Related Report
      2013 Final Research Report
  • [Presentation] 各地で栽培したspw1-cls変異を持つ閉花受粉性イネ系統の開花率と開花/閉花予測地図の試作2014

    • Author(s)
      大森伸之介、大東健太郎、岩崎亘典、佐藤弘一、岡本和之、山崎周一郎、高橋利和、望月篤、藤代淳、野村研、上野直也、細井淳、新井利直、中澤伸夫、矢ケ崎和弘、鈴木亨、佐藤秀人、加藤満、水上優子、吉田朋史、山川智大、松井崇晃、蛯谷武志、小林大樹、笹倉康弘、田野井真、矢頭治、吉田均
    • Organizer
      日本作物学会
    • Place of Presentation
      千葉市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 閉花受粉性イネの開発と利用〜イネ閉花受粉性突然変異superwoman1-cleistogamyを中心に2013

    • Author(s)
      大森伸之介、吉田均
    • Organizer
      平成25年度中央農研シンポジウム「穂・穎花(えいか)を改良するイネのデザイン育種にむけて」
    • Place of Presentation
      東京都
    • Year and Date
      2013-11-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Transgene containment using rice cleistogamous mutation2013

    • Author(s)
      Ohmori S., Tabuchi H., Yatou O., Hayashi T., Yamaguchi T., Koike S., Kuroki M., Shimizu H., Ikegaya T. and Yoshida H
    • Organizer
      MARCO-FFTC Joint International Workshop 2013 Benefits and Risks of Genetically Modified Food Crops in Asia
    • Place of Presentation
      Tsukuba
    • Related Report
      2013 Final Research Report
  • [Presentation] イネの穎花の形態と閉花性の関係2013

    • Author(s)
      吉田均、三浦孝太郎、岩崎行玄、北野英己
    • Organizer
      日本育種学会
    • Place of Presentation
      仙台市
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] イネの閉花受粉性の分子機構2013

    • Author(s)
      吉田均、大森伸之介、矢頭治
    • Organizer
      日本育種学会
    • Place of Presentation
      鹿児島市
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 閉花受粉性イネ準同質遺伝子系統の特性解析2013

    • Author(s)
      大森伸之介、秋山高、田淵宏朗、吉田均
    • Organizer
      日本育種学会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Fine-tuning the phenotype; alteration of lodicule morphology by manipulating B-class genes in rice2013

    • Author(s)
      Yoshida H., Yao S.-G., Kuroki M., Lombardo F., Kimizu M., Ohmori S., Hayashi T., Koike S. and Yatou O
    • Organizer
      JAPAN-CHINA Joint Meeting on Rice Developmental Biology
    • Place of Presentation
      Beppu
    • Related Report
      2013 Final Research Report
  • [Presentation] 閉花受粉性イネの開発と利用  ~イネ閉花受粉性突然変異superwoman1-cleistogamyを中心に~2013

    • Author(s)
      大森伸之介、吉田均
    • Organizer
      中央農研シンポジウム「穂・穎花(えいか)を改良するイネのデザイン育種にむけて」
    • Place of Presentation
      東京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Transgene containment using rice cleistogamous mutation2013

    • Author(s)
      Shinnosuke Ohmori,Hiroaki Tabuchi,Osamu Yatou,Takami Hayashi,Tomoya Yamaguchi,Setsuo Koike,Makoto Kuroki,Hiroyuki Shimizu,Tomohito Ikegaya,Hitoshi Yoshida
    • Organizer
      MARCO-FFTC Joint International Workshop 2013
    • Place of Presentation
      つくば市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 閉花受粉性イネによるイネ墨黒穂病の耕種的防除の試み2013

    • Author(s)
      石川浩司、黒田智久、岩田大介、吉田均、大森伸之介、芦澤武人
    • Organizer
      日本植物病理学会関東部会
    • Place of Presentation
      東京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Fine-tuning the phenotype; alteration of lodicule morphology by manipulating B-class genes in rice.2013

    • Author(s)
      Hitoshi Yoshida, Shan-Guo Yao, Makoto Kuroki, Fabien Lombardo, Mayumi Kimizu, Shinnosuke Ohmori, Takami Hayashi, Setsuo Koike and Osamu Yatou
    • Organizer
      JAPAN-CHINA Joint Meeting on Rice Developmental Biology
    • Place of Presentation
      別府市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Characterization of two novel rice cleistogamous mutants.2013

    • Author(s)
      Shinnosuke Ohmori, Makoto Kuroki, Setsuo Koike, Takami Hayashi, Tomoya Yamaguchi, Osamu Yatou and Hitoshi Yoshida
    • Organizer
      The Monsoon Asia Agro-Environmental Research Consortium Symposium 2012
    • Place of Presentation
      つくば市
    • Related Report
      2012 Research-status Report
  • [Presentation] Evaluation of flowering rate of rice cleistogamous mutants under different growing temperatures using the Temperature Gradient Chamber.2013

    • Author(s)
      Takami Hayashi, Hiroyuki Shimizu, Tomohito Ikegaya, Makoto Kuroki, Tomoya Yamaguchi, Shinnosuke Ohmori, Osamu Yatou, Hitoshi Yoshida and Setsuo Koike
    • Organizer
      The Monsoon Asia Agro-Environmental Research Consortium Symposium 2012
    • Place of Presentation
      つくば市
    • Related Report
      2012 Research-status Report
  • [Presentation] 閉花受粉性イネ準同質遺伝子系統の特性解析2013

    • Author(s)
      大森伸之介,秋山高,田淵宏朗,吉田均
    • Organizer
      日本育種学会 2013.3.27-28
    • Place of Presentation
      東京都
    • Related Report
      2012 Research-status Report
  • [Presentation] 北陸193号由来の新規閉花受粉性突然変異体の解析2012

    • Author(s)
      大森伸之介、黒木慎、小池説夫、林高見、山口知哉、矢頭治、吉田均
    • Organizer
      日本育種学会
    • Place of Presentation
      宇都宮市
    • Year and Date
      2012-03-30
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] 東北・関東・東海・北陸の各地で栽培したspw1-cls変異を持つ閉花受粉性イネ系統の開花率2012

    • Author(s)
      大森伸之介、佐藤弘一、岡本和之、山崎周一郎、高橋利和、望月篤、野村研、上野直也、細井淳、新井利直、鈴木亨、佐藤秀人、加藤満、水上優子、山川智大、松井崇晃、蛯谷武志、小林大樹、田野井真、矢頭治、吉田均
    • Organizer
      日本作物学会
    • Place of Presentation
      仙台市
    • Related Report
      2013 Final Research Report
  • [Presentation] Bクラス遺伝子の改変によるイネの閉花化2012

    • Author(s)
      姚善国、木水真由美、大森伸之介、吉田均
    • Organizer
      日本育種学会
    • Place of Presentation
      京都市
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization of two novel rice cleistogamous mutants2012

    • Author(s)
      Ohmori S., Kuroki M., Koike S., Hayashi T., Yamaguchi T., Yatou O. and Yoshida H
    • Organizer
      The Monsoon Asia Agro-Environmental Research Consortium Symposium 2012 Workshop 2: Biosafety and issues facing the development of Genetically Modified Crops in Monsoon Asia : Current status and Future aspects
    • Place of Presentation
      Tsukuba
    • Related Report
      2013 Final Research Report
  • [Presentation] A rice cleistogamous mutant spw1-cls1: a practical tool for transgene containment2012

    • Author(s)
      Yoshida H., Ohmori S., Tabuchi H. and Yatou O
    • Organizer
      The Monsoon Asia Agro-Environmental Research Consortium Symposium 2012 Workshop 2: Biosafety and issues facing the development of Genetically Modified Crops in Monsoon Asia : Current status and Future aspects
    • Place of Presentation
      Tsukuba
    • Related Report
      2013 Final Research Report
  • [Presentation] A rice cleistogamous mutant spw1-cls1: a practical tool for transgene containment.2012

    • Author(s)
      Hitoshi Yoshida, Shinnosuke Ohmori, Hiroaki Tabuchi and Osamu Yatou
    • Organizer
      The Monsoon Asia Agro-Environmental Research Consortium Symposium 2012
    • Place of Presentation
      つくば市
    • Related Report
      2012 Research-status Report
  • [Presentation] Bクラス遺伝子の改変によるイネの閉花化2012

    • Author(s)
      姚善国,木水真由美,大森伸之介,吉田均
    • Organizer
      日本育種学会
    • Place of Presentation
      京都市
    • Related Report
      2012 Research-status Report
  • [Presentation] 東北・関東・東海・北陸の各地で栽培したspw1-cls変異を持つ閉花受粉性イネ系統の開花率2012

    • Author(s)
      大森伸之介,佐藤弘一,岡本和之,山崎周一郎,高橋利和,望月篤,野村研,上野直也,細井淳,新井利直,鈴木亨,佐藤秀人,加藤満,水上優子,山川智大,松井崇晃,蛯谷武志,小林大樹,田野井真,矢頭治,吉田均
    • Organizer
      日本作物学会 2012.9.10-11
    • Place of Presentation
      仙台市
    • Related Report
      2012 Research-status Report
  • [Presentation] 閉花性イネ突然変異体の開花期耐冷性2012

    • Author(s)
      小池説夫,大森伸之介,山口知哉,林高見,矢頭治,吉田均
    • Organizer
      日本作物学会東北支部会
    • Place of Presentation
      秋田市
    • Related Report
      2012 Research-status Report
  • [Presentation] 生育履歴温度による閉花性イネ突然変異体の開花率変動2011

    • Author(s)
      小池説夫、林高見、山口知哉、大森伸之介、矢頭治、吉田均
    • Organizer
      日本作物学会東北支部会
    • Place of Presentation
      山形市
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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