Development of novel breeding procedure by using plant regeneration system from endosperm culture

• Project/Area Number: 23580029
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Hokkaido University
• Principal Investigator: HOSHINO YOICHIRO 北海道大学, 北方生物圏フィールド科学センター, 准教授 (50301875)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 胚乳 / ハスカップ / 倍数性育種 / 植物体再生 / 国際情報交流

Research Abstract

Plant regeneration system from endosperm culture has been developed in Haskap (Lonicera caerulea var. emphyllocalyx). The plants derived from endosperm showed same ploidy level with endosperm. This means that endosperm culture is useful for novel breeding procedure. To analyze process of endosperm culture, full length of fertilization independent endosperm (FIE) homolog was sequenced in Haskap. Expression pattern of FIE homolog was analyzed by real-time PCR during endosperm development in vivo and endosperm culture in vitro. The process of endosperm culture was discussed in expression pattern of FIE homolog.

Research Products

• [Journal Article] Interploid and intraploid hybridizations to produce polyploid Haskap (Lonicera caerulea var. emphyllocalyx) plants (2015)
  • Author(s): Miyashita T, Hoshino Y
  • Journal Title: Euphytica Volume: 201 Issue: 1 Pages: 15-27
  • DOI: 10.1007/s10681-014-1159-4
  • NAID: 120005679510
  • Peer Reviewed
• [Journal Article] Interploid and intraploid hybridizations to produce polyploid Haskap (Lonicera caerulea var. emphyllocalyx) plants (2014)
  • Author(s): Miyashita Y, Hoshino Y
  • Journal Title: Euphytica Volume: Accepted
  • NAID: 120005679510
  • Peer Reviewed
• [Journal Article] Towards Intergeneric Hybridization between Alstroemeria L. and Bomarea Mirb. (2012)
  • Author(s): Kashihara Y, Shinoda K, Araki H, Hoshino Y
  • Journal Title: Floriculture and Ornamental Biotechnology Volume: 6 Pages: 146-149
  • NAID: 120005228567
  • Peer Reviewed
• [Journal Article] In vitro culture of endosperm and its application in plant breeding : Approaches to polyploidy breeding (2011)
  • Author(s): Hoshino Y., Miyashita T., Thomas T.D
  • Journal Title: Scientia Horticulturae Volume: 130 Issue: 1 Pages: 1-8
  • DOI: 10.1016/j.scienta.2011.06.041
  • NAID: 120003260042
  • Peer Reviewed
• [Presentation] 中国および日本のハスカップ倍数性レベル、DNA含量変異およびAFLPによる多様性解析 (2013)
  • Author(s): 石山知美・下田真明・小野寺康之・青木宣明・叶玉紅・霍俊伟・孔徳剛・翁海竜・星野洋一郎
  • Organizer: 園芸学会
  • Place of Presentation: 東京農工大学
• [Presentation] 中国および日本のハスカップ倍数性レベル、DNA含量変異およびAFLPによる多様性解析 (2013)
  • Author(s): 石山知美・下田真明・小野寺康之・青木宣明・叶 玉紅・霍 俊偉・孔 徳剛・翁 海竜・星野洋一郎
  • Organizer: 園芸学会
  • Place of Presentation: 東京農工大学（小金井市）
• [Presentation] ハスカップとミヤマウグイスカグラの種間雑種の育成:GC/MS分析を利用した果実成分の評価 (2012)
  • Author(s): 下田真明・中野英樹・高橋太郎・堀廣孝・星野洋一郎
  • Organizer: 園芸学会
  • Place of Presentation: 大阪府立大学
• [Presentation] アルストロメリアの成熟花粉および発芽花粉のプロテオーム解析 (2012)
  • Author(s): 干川有吾・岡 征子・星野洋一郎
  • Organizer: 第6回日本質量分析学会・北海道談話会
  • Place of Presentation: 北海道大学（札幌市）
• [Presentation] ハスカップとミヤマウグイスカグラの種間雑種の育成：GC/MS分析を利用した果実成分の評価 (2012)
  • Author(s): 下田真明・中野英樹・高橋太郎・堀廣孝・星野洋一郎
  • Organizer: 園芸学会
  • Place of Presentation: 大阪府立大学中百舌鳥キャンパス
• [Presentation] ハスカップとミヤマウグイスカグラの種間雑種の育成:雑種個体の花および果実の特徴 (2011)
  • Author(s): 下田真明・中野英樹・高虫慧子・高橋太郎・堀廣孝・河合孝雄・星野洋一郎
  • Organizer: 園芸学会
  • Place of Presentation: 岡山大学
• [Presentation] ハスカップとミヤマウグイスカグラの種間雑種の育成：雑種個体の花および果実の特徴 (2011)
  • Author(s): 下田真明・中野英樹・高虫慧子・高橋太郎・堀廣孝・河合孝雄・星野洋一郎
  • Organizer: 園芸学会
  • Place of Presentation: 岡山大学津島キャンパス
• [Remarks]
  • URL: http://www.fsc.hokudai.ac.jp/farm/