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Development of a new cultivar production system using transgenic plants in ornamental plants

Research Project

Project/Area Number 23580037
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionNiigata University

Principal Investigator

NAKANO MASARU  新潟大学, 自然科学系, 准教授 (00262460)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords花き園芸植物 / ホトトギス類 / 形質転換体 / 遠縁種間雑種 / 胚救出 / ソマクローナル変異 / 染色体倍加 / 遠縁種間交雑
Research Abstract

White-flowered transgenic plants resulted from RNAi suppression of the CHS gene and dwarf transgenic plants resulted from overexpression of the GA2ox gene were selected as breeding materials through characterization of various transgenic plants of Tricyrtis spp. Interspecific hybrids were produced via ovule culture when dwarf transgenic plants were used as parents. Some of these hybrids showed not only dwarf phenotypes due to the transgene but also novel flower characteristics compared with the parents, indicating the possibility of interspecific hybridization using transgenic plants in the improvement programs of ornamental plants. On the other hand, induction of somaclonal variants and chromosome doubling were also examined by using embryogenic callus cultures of transgenic plants. Detailed characterization of callus culture-derived plants are now in progress.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (16 results)

All 2014 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] Overexpression of the gibberellin 20-oxidase or gibberellin 3-oxidase gene from Torenia fournieri affects plant morphology in transgenic Tricyrtis sp2014

    • Author(s)
      Masahiro Otani、Shuhei Meguro、Haruka Gondaira、Megumi Hayashi、Misaki Saito、Dong-Sheng Han、Masaru Nakano
    • Journal Title

      Acta Horticulturae

      Volume: 1025巻

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interspecific hybridization between Tricyrtis flava and T, formosana via ovule culture2014

    • Author(s)
      Haruka Tasaki、Hiroki Takagi、Hanae Otsubo、Chika Hatakeyama、Yusaku Saito、Eriko Oka、Junji Amano、Dong-Sheng Han、Masaru Nakano
    • Journal Title

      Acta Horticulturae

      Volume: 1025巻

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of the gibberellin 2-oxidase gene from Torenia fournieri induces dwarf phenotypes in the liliaceous monocotyledon Tricyrtis sp.2013

    • Author(s)
      Masahiro Otani, Shuhei Meguro, Haruka Gondaira, Megumi Hayashi, Misaki Saito, Dong-Sheng Han, Phithak Inthima, Kanyaratt Supaibulwatana, Shiro Mori, Yusuke Jikumaru, Yuji Kamiya, Tuoping Li, Tomoya Niki, Takaaki Nishijima, Masaji Koshioka and Masaru Nakano
    • Journal Title

      Journal of Plant Physiology

      Volume: 170 Issue: 16 Pages: 1416-1423

    • DOI

      10.1016/j.jplph.2013.05.002

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Flower color alteration in the liliaceous ornamental Tricyrtis sp by RNA interference- mediated suppression of the chalcone synthase gene2012

    • Author(s)
      Yukiko Kamiishi、Masahiro Otani、Hiroki Takagi、Dong-Sheng Han、Shiro Mori、Fumi Tatsuzawa、Hiroaki Okuhara、Hitoshi Kobayashi、Masaru Nakano
    • Journal Title

      Molecular Breeding

      Volume: 30巻

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Flower color alteration in the liliaceous ornamental Tricyrtis sp. by RNA interference-mediated suppression of the chalcone synthase gene2012

    • Author(s)
      Yukiko Kamiishi, Masahiro Otani, Hiroki Takagi, Dong-Sheng Han, Shiro Mori, Fumi Tatsuzawa, Hiroaki Okuhara, Hitoshi Kobayashi and Masaru Nakano
    • Journal Title

      Molecular Breeding

      Volume: 30 Issue: 2 Pages: 671-680

    • DOI

      10.1007/s11032-011-9653-z

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Horticultural characterization of a tetraploid transgenic plant of Tricyrtis sp. overexpressing the gibberellin 2-oxidase gene from Torenia fournieri2013

    • Author(s)
      Masaru Nakano、Mitsuyo Ishibe、Masahiro Otani、Shuhei Meguro、Haruka Gondaira、Megumi Hayashi、Misaki Saito、Dong-Sheng Han、Shiro Mori、Tomoya Niki、Takaaki Nishijima、Masaji Koshioka
    • Organizer
      8th International Symposium on In Vitro Culture and Horticultural Breeding
    • Place of Presentation
      コインブラ、ポルトガル
    • Year and Date
      2013-07-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Horticultural characterization of a tetraploid transgenic plant of Tricyrtis sp. overexpressing the gibberellin 2-oxidase gene from Torenia fournieri2013

    • Author(s)
      Masaru Nakano, Mitsuyo Ishibe, Masahiro Otani, Shuhei Meguro, Haruka Gondaira, Megumi Hayashi, Misaki Saito, Dong-Sheng Han, Shiro Mori, Tomoya Niki, Takaaki Nishijima and Masaji Koshioka
    • Organizer
      8th International Symposium on In Vitro Culture and Horticultural Breeding
    • Place of Presentation
      コインブラ,ポルトガル
    • Related Report
      2013 Annual Research Report
  • [Presentation] 胚珠培養によるキバナノホトトギスとタイワンホトトギス間の種間雑種の作出2012

    • Author(s)
      石部万葉、田崎晴香、高木宏樹、韓東生、神戸敏成、中野優
    • Organizer
      園芸学会北陸支部大会
    • Place of Presentation
      朱鷺メッセ、新潟市
    • Year and Date
      2012-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Overexpression of the gibberellin 20-oxidase or gibberellin 3-oxidase gene from Torenia fournieri affects plant morphology in transgenic Tricyrtis sp2012

    • Author(s)
      Masahiro Otani、Shuhei Meguro、Haruka Gondaira、Megumi Hayashi、Misaki Saito、Shiro Mori、Dong-Sheng Han、Tuoping Li、Tomoya Niki、Takaaki Nishijima、Masaji Koshioka、Masaru Nakano
    • Organizer
      ISHS Symposia at Royal Flora 2011
    • Place of Presentation
      チェンマイ、タイ王国
    • Year and Date
      2012-01-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Interspecific hybridization between Tricyrtis flava and T2012

    • Author(s)
      Haruka Tasaki、Hiroki Takagi、Hanae Otsubo、Chika Hatakeyama、Yusaku Saito、Eriko Oka、Junji Amano、Yusuke Watanabe、Dong-Sheng Han、Toshinari Godo、Masaru Nakano
    • Organizer
      formosana via ovule culture、ISHS Symposia at Royal Flora 2011
    • Place of Presentation
      チェンマイ、タイ王国
    • Year and Date
      2012-01-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization of transgenic Agapanthus praecox spp2012

    • Author(s)
      Nao Okuzumi、Masahiro Otani、Hanae Otsubo、Shuhei Meguro、Yoshihiro Hara、Hiroto Umehara、Mika Igarashi、Dong-Sheng Han、Yoichiro Hoshino、Akira Kanno、Masaru Nakano
    • Organizer
      orientalis plants ectopically expressing the class B MADS-box genes、ISHS Symposia at Royal Flora 2011
    • Place of Presentation
      チェンマイ、タイ王国
    • Year and Date
      2012-01-10
    • Related Report
      2013 Final Research Report
  • [Presentation] 胚珠培養によるキバナノホトトギスとタイワンホトトギス間の種間雑種の作出2012

    • Author(s)
      石部万葉・田崎晴香・高木宏樹・韓 東生・神戸敏成・中野 優
    • Organizer
      園芸学会北陸支部
    • Place of Presentation
      新潟市朱鷺メッセ
    • Related Report
      2012 Research-status Report
  • [Presentation] Overexpression of the gibberellin 20-oxidase or gibberellin 3-oxidase gene from Torenia fournieri affects plant morphology in transgenic Tricyrtis sp.2012

    • Author(s)
      Masahiro OTANI, Shuhei MEGURO, Haruka GONDAIRA, Megumi HAYASHI, Misaki SAITO, Shiro MORI, Dong-Sheng HAN, Tuoping LI, Tomoya NIKI, Takaaki NISHIJIMA, Masaji KOSHIOKA and Masaru NAKANO
    • Organizer
      ISHS Symposia at Royal Flora 2011
    • Place of Presentation
      チェンマイ,タイ王国
    • Related Report
      2011 Research-status Report
  • [Presentation] Interspecific hybridization between Tricyrtis flava and T. formosana via ovule culture2012

    • Author(s)
      Haruka TASAKI, Hiroki TAKAGI, Hanae OTSUBO, Chika HATAKEYAMA, Yusaku SAITO, Eriko OKA, Junji AMANO, Yusuke WATANABE, Dong-Sheng HAN, Toshinari GODO and Masaru NAKANO
    • Organizer
      ISHS Symposia at Royal Flora 2011
    • Place of Presentation
      チェンマイ,タイ王国
    • Related Report
      2011 Research-status Report
  • [Presentation] Characterization of transgenic Agapanthus praecox spp. orientalis plants ectopically expressing the class B MADS-box genes2012

    • Author(s)
      Nao OKUZUMI, Masahiro OTANI, Hanae OTSUBO, Shuhei MEGURO, Yoshihiro HARA, Hiroto UMEHARA, Mika IGARASHI, Dong-Sheng HAN, Yoichiro HOSHINO, Akira KANNO and Masaru NAKANO
    • Organizer
      ISHS Symposia at Royal Flora 2011
    • Place of Presentation
      チェンマイ,タイ王国
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.agr.niigata-u.ac.jp/~mnakano/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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