Development of a new cultivar production system using transgenic plants in ornamental plants

• Project/Area Number: 23580037
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Niigata University
• Principal Investigator: NAKANO MASARU 新潟大学, 自然科学系, 准教授 (00262460)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 花き園芸植物 / ホトトギス類 / 形質転換体 / 遠縁種間雑種 / 胚救出 / ソマクローナル変異 / 染色体倍加 / 遠縁種間交雑

Research Abstract

White-flowered transgenic plants resulted from RNAi suppression of the CHS gene and dwarf transgenic plants resulted from overexpression of the GA2ox gene were selected as breeding materials through characterization of various transgenic plants of Tricyrtis spp. Interspecific hybrids were produced via ovule culture when dwarf transgenic plants were used as parents. Some of these hybrids showed not only dwarf phenotypes due to the transgene but also novel flower characteristics compared with the parents, indicating the possibility of interspecific hybridization using transgenic plants in the improvement programs of ornamental plants. On the other hand, induction of somaclonal variants and chromosome doubling were also examined by using embryogenic callus cultures of transgenic plants. Detailed characterization of callus culture-derived plants are now in progress.

Research Products

• [Journal Article] Overexpression of the gibberellin 20-oxidase or gibberellin 3-oxidase gene from Torenia fournieri affects plant morphology in transgenic Tricyrtis sp (2014)
  • Author(s): Masahiro Otani、Shuhei Meguro、Haruka Gondaira、Megumi Hayashi、Misaki Saito、Dong-Sheng Han、Masaru Nakano
  • Journal Title: Acta Horticulturae Volume: 1025巻
  • Peer Reviewed
• [Journal Article] Interspecific hybridization between Tricyrtis flava and T, formosana via ovule culture (2014)
  • Author(s): Haruka Tasaki、Hiroki Takagi、Hanae Otsubo、Chika Hatakeyama、Yusaku Saito、Eriko Oka、Junji Amano、Dong-Sheng Han、Masaru Nakano
  • Journal Title: Acta Horticulturae Volume: 1025巻
  • Peer Reviewed
• [Journal Article] Overexpression of the gibberellin 2-oxidase gene from Torenia fournieri induces dwarf phenotypes in the liliaceous monocotyledon Tricyrtis sp. (2013)
  • Author(s): Masahiro Otani, Shuhei Meguro, Haruka Gondaira, Megumi Hayashi, Misaki Saito, Dong-Sheng Han, Phithak Inthima, Kanyaratt Supaibulwatana, Shiro Mori, Yusuke Jikumaru, Yuji Kamiya, Tuoping Li, Tomoya Niki, Takaaki Nishijima, Masaji Koshioka and Masaru Nakano
  • Journal Title: Journal of Plant Physiology Volume: 170 Issue: 16 Pages: 1416-1423
  • DOI: 10.1016/j.jplph.2013.05.002
  • Peer Reviewed
• [Journal Article] Flower color alteration in the liliaceous ornamental Tricyrtis sp by RNA interference- mediated suppression of the chalcone synthase gene (2012)
  • Author(s): Yukiko Kamiishi、Masahiro Otani、Hiroki Takagi、Dong-Sheng Han、Shiro Mori、Fumi Tatsuzawa、Hiroaki Okuhara、Hitoshi Kobayashi、Masaru Nakano
  • Journal Title: Molecular Breeding Volume: 30巻
  • Peer Reviewed
• [Journal Article] Flower color alteration in the liliaceous ornamental Tricyrtis sp. by RNA interference-mediated suppression of the chalcone synthase gene (2012)
  • Author(s): Yukiko Kamiishi, Masahiro Otani, Hiroki Takagi, Dong-Sheng Han, Shiro Mori, Fumi Tatsuzawa, Hiroaki Okuhara, Hitoshi Kobayashi and Masaru Nakano
  • Journal Title: Molecular Breeding Volume: 30 Issue: 2 Pages: 671-680
  • DOI: 10.1007/s11032-011-9653-z
  • Peer Reviewed
• [Presentation] Horticultural characterization of a tetraploid transgenic plant of Tricyrtis sp. overexpressing the gibberellin 2-oxidase gene from Torenia fournieri (2013)
  • Author(s): Masaru Nakano、Mitsuyo Ishibe、Masahiro Otani、Shuhei Meguro、Haruka Gondaira、Megumi Hayashi、Misaki Saito、Dong-Sheng Han、Shiro Mori、Tomoya Niki、Takaaki Nishijima、Masaji Koshioka
  • Organizer: 8th International Symposium on In Vitro Culture and Horticultural Breeding
  • Place of Presentation: コインブラ、ポルトガル
  • Year and Date: 2013-07-05
• [Presentation] Horticultural characterization of a tetraploid transgenic plant of Tricyrtis sp. overexpressing the gibberellin 2-oxidase gene from Torenia fournieri (2013)
  • Author(s): Masaru Nakano, Mitsuyo Ishibe, Masahiro Otani, Shuhei Meguro, Haruka Gondaira, Megumi Hayashi, Misaki Saito, Dong-Sheng Han, Shiro Mori, Tomoya Niki, Takaaki Nishijima and Masaji Koshioka
  • Organizer: 8th International Symposium on In Vitro Culture and Horticultural Breeding
  • Place of Presentation: コインブラ，ポルトガル
• [Presentation] 胚珠培養によるキバナノホトトギスとタイワンホトトギス間の種間雑種の作出 (2012)
  • Author(s): 石部万葉、田崎晴香、高木宏樹、韓東生、神戸敏成、中野優
  • Organizer: 園芸学会北陸支部大会
  • Place of Presentation: 朱鷺メッセ、新潟市
  • Year and Date: 2012-12-04
• [Presentation] Overexpression of the gibberellin 20-oxidase or gibberellin 3-oxidase gene from Torenia fournieri affects plant morphology in transgenic Tricyrtis sp (2012)
  • Author(s): Masahiro Otani、Shuhei Meguro、Haruka Gondaira、Megumi Hayashi、Misaki Saito、Shiro Mori、Dong-Sheng Han、Tuoping Li、Tomoya Niki、Takaaki Nishijima、Masaji Koshioka、Masaru Nakano
  • Organizer: ISHS Symposia at Royal Flora 2011
  • Place of Presentation: チェンマイ、タイ王国
  • Year and Date: 2012-01-10
• [Presentation] Interspecific hybridization between Tricyrtis flava and T (2012)
  • Author(s): Haruka Tasaki、Hiroki Takagi、Hanae Otsubo、Chika Hatakeyama、Yusaku Saito、Eriko Oka、Junji Amano、Yusuke Watanabe、Dong-Sheng Han、Toshinari Godo、Masaru Nakano
  • Organizer: formosana via ovule culture、ISHS Symposia at Royal Flora 2011
  • Place of Presentation: チェンマイ、タイ王国
  • Year and Date: 2012-01-10
• [Presentation] Characterization of transgenic Agapanthus praecox spp (2012)
  • Author(s): Nao Okuzumi、Masahiro Otani、Hanae Otsubo、Shuhei Meguro、Yoshihiro Hara、Hiroto Umehara、Mika Igarashi、Dong-Sheng Han、Yoichiro Hoshino、Akira Kanno、Masaru Nakano
  • Organizer: orientalis plants ectopically expressing the class B MADS-box genes、ISHS Symposia at Royal Flora 2011
  • Place of Presentation: チェンマイ、タイ王国
  • Year and Date: 2012-01-10
• [Presentation] 胚珠培養によるキバナノホトトギスとタイワンホトトギス間の種間雑種の作出 (2012)
  • Author(s): 石部万葉・田崎晴香・高木宏樹・韓 東生・神戸敏成・中野 優
  • Organizer: 園芸学会北陸支部
  • Place of Presentation: 新潟市朱鷺メッセ
• [Presentation] Overexpression of the gibberellin 20-oxidase or gibberellin 3-oxidase gene from Torenia fournieri affects plant morphology in transgenic Tricyrtis sp. (2012)
  • Author(s): Masahiro OTANI, Shuhei MEGURO, Haruka GONDAIRA, Megumi HAYASHI, Misaki SAITO, Shiro MORI, Dong-Sheng HAN, Tuoping LI, Tomoya NIKI, Takaaki NISHIJIMA, Masaji KOSHIOKA and Masaru NAKANO
  • Organizer: ISHS Symposia at Royal Flora 2011
  • Place of Presentation: チェンマイ，タイ王国
• [Presentation] Interspecific hybridization between Tricyrtis flava and T. formosana via ovule culture (2012)
  • Author(s): Haruka TASAKI, Hiroki TAKAGI, Hanae OTSUBO, Chika HATAKEYAMA, Yusaku SAITO, Eriko OKA, Junji AMANO, Yusuke WATANABE, Dong-Sheng HAN, Toshinari GODO and Masaru NAKANO
  • Organizer: ISHS Symposia at Royal Flora 2011
  • Place of Presentation: チェンマイ，タイ王国
• [Presentation] Characterization of transgenic Agapanthus praecox spp. orientalis plants ectopically expressing the class B MADS-box genes (2012)
  • Author(s): Nao OKUZUMI, Masahiro OTANI, Hanae OTSUBO, Shuhei MEGURO, Yoshihiro HARA, Hiroto UMEHARA, Mika IGARASHI, Dong-Sheng HAN, Yoichiro HOSHINO, Akira KANNO and Masaru NAKANO
  • Organizer: ISHS Symposia at Royal Flora 2011
  • Place of Presentation: チェンマイ，タイ王国
• [Remarks]
  • URL: http://www.agr.niigata-u.ac.jp/~mnakano/index.html