Biosynthesis of aroma compounds in grape berries: development of a novel practice for aroma compounds in wine

• Project/Area Number: 23580038
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: University of Yamanashi
• Principal Investigator: SUZUKI Shunji 山梨大学, 総合研究部, 准教授 (60372728)
• Co-Investigator(Kenkyū-buntansha): FUJITA Keiko 県立広島大学, 生命環境学部, 助教 (50467726)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ブドウ / 香り / 3-メルカプトヘキサノール / 環境ストレス / 日周性 / ワイン / ストレス応答 / グルタチオン / 3-メルカプトヘキサノール前駆体 / 甲州 / ナイトハーベスト / グルタチン-S-トランスフェラーゼ / γ-グルタミルトランスフェラーゼ / 栽培技術

Outline of Final Research Achievements

S-(3-hexan-1-ol)cysteinylglycine, which are proposed as the flavor precursors of 3-mercaptohexan-1-ol (3MH) in grape berries, was detected using liquid chromatography-tandem mass spectrometry. Synthesis of 3MH precursors was catalyzed by glutathione S-transferases (VvGST3 and VvGST4). Accumulation of 3MH precursors was enhanced in berries treated with various environmental stresses in accordance with upregulation of VvGST3 and VvGST4 gene expression. 3MH precursors were highly accumulated at 16-18 weeks post-flowering. The accumulation of 3MH precursors in berries increased in early morning and decreased during the day.

Research Products

• [Journal Article] Impact of harvest timing on the concentration of 3-mercaptohexan-1-ol precursors in Vitis vinifera grape berries. (2012)
  • Author(s): Kobayashi, H., Matsuyama, S., Takase, H., Sasaki, K., Suzuki, S., Takata, R. and Saito, H.
  • Journal Title: American Journal of Enology and Viticulture Volume: 63 Issue: 4 Pages: 544-548
  • DOI: 10.5344/ajev.2012.12051
  • Peer Reviewed
• [Presentation] ブドウ収穫タイミングの違いが前駆体3MH（3mercaptohexan-1-ol precursors）濃度へ与える影響 (2012)
  • Author(s): 小林弘憲、松山周平、高瀬秀樹、佐々木佳菜子、高田良二、鈴木 俊二、齋藤浩
  • Organizer: 日本ブドウ・ワイン学会
  • Place of Presentation: レンブラントホテル大分（大分）
• [Presentation] 甲州ブドウにおける気象条件および栽培方法の違いが果実成分へ与える影響
  • Author(s): 小林弘憲、松山周平、高瀬秀樹、佐々木佳菜子、ら
  • Organizer: 日本ブドウ・ワイン学会
  • Place of Presentation: 長野県塩尻市
• [Book] Physiological and Molecular Characterization of.. (2012)
  • Author(s): Suzuki, S. and Kobayashi, H.
  • Total Pages: 39
  • Publisher: Nova Science Publishers Inc
• [Remarks] 山梨大学ワイン科学研究センター果実遺伝子工学研究部門
  • URL: http://www.wine.yamanashi.ac.jp/fruitgenetic/index.htm
• [Remarks] 日本経済新聞電子版
  • URL: http://www.nikkei.com/article/DGXNZO46502370U2A920C1TJM000/
• [Remarks] マイナビニュース
  • URL: http://news.mynavi.jp/news/2012/11/15/092/
• [Remarks] キリン株式会社ホームページ
  • URL: http://www.kirin.co.jp/company/news/2012/12047.html
• [Remarks] 山梨大学研究者総覧
  • URL: http://erdb.yamanashi.ac.jp/rdb/A_DispInfo.Scholar?ID=1B250C282E24FAFC
• [Remarks] 研究室ホームページ
  • URL: http://www.wine.yamanashi.ac.jp/fruitgenetic/index.htm
• [Remarks]
  • URL: http://erdb.yamanashi.ac.jp/rdb/A_DispInfo.Scholar/0/1B250C282E24FAFC.html