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Development of genetic statistics verification base in effect of breeding based on allele analysis

Research Project

Project/Area Number 23580055
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

FUJII Hiroshi  独立行政法人農業・食品産業技術総合研究機構, 果樹研究所カンキツ研究領域, 主任研究員 (00355398)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywordsアレル / カンキツ / カロテノイド / 遺伝統計学 / SNP / 育種 / 遺伝子 / ゲノム / βークリプトキサンチン / アリル / 多型
Research Abstract

Carotenoids have healthy functionality. The high content of carotenoids in citrus fruit is an important breeding objective. The content of the carotenoid is different in each cultivar. However the dominant cultivars in Japnan originates in the ancestral cultivar of 14. Therefore, the number of allele is limited to 28. The purpose of this study is that the allele type of 28 is shown in eight carotenoid metabolic enzyme genes and three transcription factor candidate genes that control them, and the allele combination related to the high content of carotenoids is clarified.
Consequently, the sequences of 11 genes related to the content of the carotenoid was decided in the ancestral cultivar of 14. The allele type was decided by the SNP analysis.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (4 results)

All 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] Construction of a citrus framework genetic map anchored by 708 gene-based markers.2014

    • Author(s)
      Shimada, T., H. Fujii, T. Endo, T. Ueda, A. Sugiyama, M. Nakano, M. Kita, T. Yoshioka, T. Shimizu, H. Nesumi, Y. Ikoma, T. Moriguchi, M. Omura
    • Journal Title

      Tree Genetics and Genomes

      Volume: 未定 Issue: 4 Pages: 1001-1013

    • DOI

      10.1007/s11295-014-0738-9

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] High-throughput genotyping in citrus accessions using an SNP genotyping array2013

    • Author(s)
      Fujii、H.、T. Shimada、K. Nonaka、M. Kita、T. Kuniga、T. Endo、Y. Ikoma、M. Omura
    • Journal Title

      Tree Genetics and Genomes

      Volume: 9巻 Issue: 1 Pages: 145-153

    • DOI

      10.1007/s11295-012-0542-3

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] QTLによるUDP-グルコース:リモノイド糖転移酵素遺伝子の発現に関与する転写因子候補の探索2014

    • Author(s)
      杉山愛子、原田健太郎、島田武彦、藤井浩、遠藤朋子、喜多正幸、吉岡照高、生駒吉識、大村三男
    • Organizer
      日本育種学会第125回講演会
    • Year and Date
      2014-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] eQTLによるUDP-グルコース:リモノイド糖転移酵素遺伝子の発現に関与する転写因子候補の探索2014

    • Author(s)
      杉山愛子,原田健太郎,島田武彦,藤井浩,遠藤朋子,喜多正幸,吉岡照高,生駒吉識,大村三男
    • Organizer
      日本育種学会第125回講演会
    • Place of Presentation
      東北大学
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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