| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
To develop an objective method for evaluating the freshness of vegetables, the specific genes up or down regulated during storage were identified, and using them to estimate the freshness of vegetables were investigated.
About 500 genes, changing expression during storage of spinach, broccoli and lettuce were cloned and sequenced. Of these genes, combinations of specific genes were detectable by multiplex RT-PCR, which was found to be good markers standing for freshness. A pair of degenerated primers from spinach cysteine protease that amplify a 506 bp fragment were found to be applicable for estimating the freshness on different species of plants such as spinach and broccoli.
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