Research Project
Grant-in-Aid for Scientific Research (C)
Between pathogen and host, attack and counter-attack impose strong reciprocal selection on the involved organisms, leading to the development of arms race evolutionary dynamics. Here we show that Magnaporthe oryzae avirulence gene AVR-Pik and cognate rice resistance (R-) gene Pik are highly variable with multiple alleles differing by DNA replacements causing amino acid changes. There is a tight recognition specificity of AVR-Pik alleles by different Pik alleles. We found that AVR-Pik physically binds the N-terminal coiled-coil (CC) domain of Pik in yeast 2-hybrid assay as well as in in-planta co-immunoprecipitation assay. This binding specificity correlates the recognition specificity between AVR and R-genes. We propose that AVR-Pik and Pik are locked up in arms race co-evolution that is driven by their direct physical interactions.
All 2014 2013 2012 2011 Other
All Journal Article (9 results) (of which Peer Reviewed: 8 results) Presentation (14 results) Book (3 results)
EMBO journal
Volume: (in press)
PLoS One
Volume: 8 Issue: 7 Pages: e68529-e68529
10.1371/journal.pone.0068529
New Phytologist
Volume: 200 Issue: 1 Pages: 276-283
10.1111/nph.12369
Plant Cell
Volume: 25 Issue: 4 Pages: 1463-1481
10.1105/tpc.112.107201
Nature Biotechnology
Volume: 30 Issue: 2 Pages: 174-178
10.1038/nbt.2095
Genes & Genetic Systems
Volume: 87 Issue: 3 Pages: 169-179
10.1266/ggs.87.169
10030945208
J. Plant Biochem. Biotechnol.
Volume: 21 Issue: S1 Pages: 10-14
10.1007/s13562-012-0133-2
The Plant Journal
Volume: 72 Issue: 6 Pages: 894-907
10.1111/j.1365-313x.2012.05110.x
Plant Journal
Volume: 66 Issue: 3 Pages: 467-479
10.1111/j.1365-313x.2011.04502.x