Elucidation of the mechanisme of specific recognition between rice and rice blast

• Project/Area Number: 23580065
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: KANZAKI Hiroyuki 公益財団法人岩手生物工学研究センター, 生命科学研究部, 研究専門員 (10390882)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: イネ / いもち病菌 / 非病原力遺伝子AVR-Pik / 認識特異性 / タンパク質相互作用 / Dual抵抗性遺伝子 / Effector / いもち病抵抗性遺伝子Pik / 非病原力遺伝子AvrPik / effector / 非病原力遺伝子 / 抵抗性遺伝子 / アリル / 共進化

Research Abstract

Between pathogen and host, attack and counter-attack impose strong reciprocal selection on the involved organisms, leading to the development of arms race evolutionary dynamics. Here we show that Magnaporthe oryzae avirulence gene AVR-Pik and cognate rice resistance (R-) gene Pik are highly variable with multiple alleles differing by DNA replacements causing amino acid changes. There is a tight recognition specificity of AVR-Pik alleles by different Pik alleles. We found that AVR-Pik physically binds the N-terminal coiled-coil (CC) domain of Pik in yeast 2-hybrid assay as well as in in-planta co-immunoprecipitation assay. This binding specificity correlates the recognition specificity between AVR and R-genes. We propose that AVR-Pik and Pik are locked up in arms race co-evolution that is driven by their direct physical interactions.

Research Products

• [Journal Article] The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to confer disease resistance (2014)
  • Author(s): Césari, S., Kanzaki, H., Fujiwara, T.(co-first authors), Bernoux, M., Chalvon, V., Kawano, Y., Shimamoto, K., Dodds P., Terauchi, R., Kroj, T
  • Journal Title: EMBO journal Volume: (in press)
• [Journal Article] MutMap+ : Genetic mapping and mutant identification without crossing in rice (2013)
  • Author(s): Fekih, R., Takagi, H., Tamiru, M., Abe, A., Natsume, S., et al.
  • Journal Title: PLoS One Volume: 8 Issue: 7 Pages: e68529-e68529
  • DOI: 10.1371/journal.pone.0068529
  • Peer Reviewed
• [Journal Article] MutMap-Gap:whole-genome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gne Pii (2013)
  • Author(s): Takagi,H., Uemura,A., Yaegashi,H., Tamiru, M., Abe, A., Mitsuoka,C., Utsushi,H., Natsume,S., Kanzaki,H., Matsumura,H., Saitoh,H., Yoshida,K., Cano,L.M., Kamoun,S., Terauchi,R.
  • Journal Title: New Phytologist Volume: 200 Issue: 1 Pages: 276-283
  • DOI: 10.1111/nph.12369
  • Peer Reviewed
• [Journal Article] The rice resistance protein pair RGA4/RGA5 recognize the Magnaporthe oryzae effectors AVR-Pia and AVR-CO39 by direct binding. (2013)
  • Author(s): Cesari, S., Thilliez, G., Ribot, C., Chalvon, V., Michel, C., Jauneau, A., Rivas, S., Alaux, L., Kanzaki, H., Okuyama, Y., Morel, J.B., Fournier, E., Terauchi, R., Kroj, T.
  • Journal Title: Plant Cell Volume: 25 Issue: 4 Pages: 1463-1481
  • DOI: 10.1105/tpc.112.107201
  • Peer Reviewed
• [Journal Article] Genome sequencing reveals agronomically important loci in rice using MutMap (2013)
  • Author(s): Abe Akira, Kosugi Shunichi, Yoshida Kentaro, Natsume Satoshi, Takagi Hiroki, Kanzaki Hiroyuki, Matsumura Hideo, (他 7 名 , 7 番 目)
  • Journal Title: Nature Biotechnology Volume: 30 Issue: 2 Pages: 174-178
  • DOI: 10.1038/nbt.2095
  • Peer Reviewed
• [Journal Article] Mutation in <i>OsLMS</i>, a gene encoding a protein with two double-stranded RNA binding motifs, causes lesion mimic phenotype and early senescence in rice (<i>Oryza sativa</i> L.) (2012)
  • Author(s): Undan JR, Tamiru M, Abe A, Yoshida K, Kosugi S, Takagi H, Yoshida K, Kanzaki H, Saitoh H, Fekih R, Sharma S, Undan J, Yano M, Terauchi R.
  • Journal Title: Genes & Genetic Systems Volume: 87 Issue: 3 Pages: 169-179
  • DOI: 10.1266/ggs.87.169
  • NAID: 10030945208
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed
• [Journal Article] Whole genome sequencing and future breeding of rice. (2012)
  • Author(s): Terauchi, R., Abe, A., Takagi, H., Yoshida, K., Kosugi, S., Natsume, S., Yaegashi, H, Kanzaki, H., Matsumura, H., Mitsuoka, C., Utsushi, H., Tamiru, M.
  • Journal Title: J. Plant Biochem. Biotechnol. Volume: 21 Issue: S1 Pages: 10-14
  • DOI: 10.1007/s13562-012-0133-2
  • Peer Reviewed
• [Journal Article] Arms race co-evolution of Magnaporthe oryzae AVR-Pik and rice Pik genes driven by their physical interactions. (2012)
  • Author(s): Kanzaki H, Yoshida K, Saitoh H, Fujisaki K, Hirabuchi A, Alaux L, Fournier E, Tharreau D, Terauchi R.
  • Journal Title: The Plant Journal Volume: 72 Issue: 6 Pages: 894-907
  • DOI: 10.1111/j.1365-313x.2012.05110.x
  • Peer Reviewed
• [Journal Article] A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes. (2011)
  • Author(s): Okuyama Yudai, Kanzaki Hiroyuki, Abe Akira, Yoshida Kentaro, Tamiru Mlumei, Saitoh Hiromasa, Fujibe Takahiro, Matsumura Hideo,(他 6 名)
  • Journal Title: Plant Journal Volume: 66 Issue: 3 Pages: 467-479
  • DOI: 10.1111/j.1365-313x.2011.04502.x
  • Peer Reviewed
• [Presentation] Mark Banfield、Sophien Kamoun、寺内良平いもち病菌(Magnaporthe oryzae)非病原力遺伝子AVR-Pikと相互作用するイネタンパク質の同定 (2014)
  • Author(s): 神崎洋之、斉藤宏昌、藤崎恒喜、小林光智衣、伊藤和江、神崎英子、三岡周子
  • Organizer: 平成26年度日本植物生理学会
  • Place of Presentation: 富山市
• [Presentation] いもち病菌（Magnaporthe oryzae）非病原力遺伝子AVR-Pikと相互作用するイネタンパク質の同定 (2014)
  • Author(s): 神崎洋之、斉藤宏昌、藤崎恒喜、小林光智衣、伊藤和江、神崎英子、三岡周子、Mark Banfield、Sophien Kamoun、寺内良平
  • Organizer: 平成2６年度日本植物生理学会
  • Place of Presentation: 富山大学五福キャンパス （富山市）
• [Presentation] Rice proteins that interact with the M. oryzae avirulence effector Avr-Pik (2013)
  • Author(s): Kanzaki, H., Sautoh, H., Fujisaki, K., Kobayashi, M., Ito, K., Kanzaki, E., Mitsuoka, C., Banfield, M., Kamoun, S., Terauchi, R
  • Organizer: 2013-International Rice Blast Conference
  • Place of Presentation: 韓国済州島
  • Year and Date: 2013-08-20
• [Presentation] Rice proteins that interact with the M.oryzae avirulence effector Avr-Pik (2013)
  • Author(s): Hiroyuki Kanzaki, Hiromasa Saitoh, Koki Fujisaki,Michie Kobayashi, Kazue Ito, Eiko Kanzaki, Chikako Mitsuoka,Mark Banfield, Sophien Kamoun
  • Organizer: 2013 International Rice Blast Conference
  • Place of Presentation: Ramada Plaza Jeju Hotel, Jejudo, South Korea（韓国、済州島）
• [Presentation] いもち病菌非病原力遺伝子AVR- Pikとイネいもち病抵抗性遺伝子Pikは、直接的タンパク質相互作用によって認識する (2012)
  • Author(s): 神崎洋之、吉田健太郎、平渕亜紀子、寺内良平
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-03-29
• [Presentation] いもち病菌非病原力遺伝子AVR-Pikが認識する新規イネ遺伝子の単離 (2012)
  • Author(s): 神崎洋之・斉藤宏昌・藤崎恒喜・寺内良平
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜市
• [Presentation] Arms race co-evolution of Magnaporthe oryzae AVR gene and rice R genes driven by their physical interactions (2012)
  • Author(s): Kanzaki, H., Yoshida, K., Saitoh, H., Fujisaki, K., Hirabuchi, A., Allaux, L., Fournier. E., Tharreau. D., Terauchi, R
  • Organizer: 30th New Phytologist Symposium～Immunomodulation by plant-associated organisms
  • Place of Presentation: California米国
• [Presentation] The direct protein-protein interaction results in the arms race co-evolution between Magnaporthe oryzae AVR-Pik and rice Pik (2012)
  • Author(s): Kanzaki, H., Yoshida, K., Saitoh, H., Hirabuchi, A., Allaux, L., Fournier. E., Tharreau. D., Terauchi, R
  • Organizer: XI International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都市
• [Presentation] いもち病菌非病原力遺伝子AVR-Pikとイネいもち病抵抗性遺伝子Pikは、直接的タンパク質相互作用によって認識する (2012)
  • Author(s): 神崎 洋之・吉田 健太郎・平渕亜紀子・寺内 良平
  • Organizer: 日本植物病理学会
  • Place of Presentation: 福岡国際会議場（福岡市）
• [Presentation] The direct protein-protein interaction results in the arms race co-evolution between Magnaporthe oryzae AVR-Pik and rice Pik (2012)
  • Author(s): Hiroyuki Kanzaki, Kentaro Yoshida, Hiromasa Saitoh, Akiko Hirabuchi, Ludovic Allaux, Elisabeth Fournier, Didier Tharreau, Ryohei Terauchi
  • Organizer: XI International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: Kyoto International Confernce Center（京都市）
• [Presentation] Arms race co-evolution of Magnaporthe oryzae AVR gene and rice R genes driven by their physical interactions (2012)
  • Author(s): Hiroyuki Kanzaki, Kentaro Yoshida, Hiromasa Saitoh, Koki Fujisaki, Akiko Hirabuchi, Ryohei Terauchi
  • Organizer: 30th New Phytologist Symposium ～ Immunomodulation by plant-associated organisms
  • Place of Presentation: Stanford Sierra Conference Center （California, USA）
• [Presentation] いもち病菌非病原力遺伝子AVR- Pikとイネいもち病抵抗性遺伝子Pikにおけるアリル間認識特異性 (2011)
  • Author(s): 神崎洋之、吉田健太郎、寺内良平
  • Organizer: 日本育種学会第120回講演会秋季大会
  • Place of Presentation: 福岡市
  • Year and Date: 2011-09-23
• [Presentation] いもち病菌非病原力遺伝子AVR-Pikとイネいもち病抵抗性遺伝子Pikにおけるアリル間認識特異性
  • Author(s): 神崎 洋之、吉田 健太郎、寺内 良平
  • Organizer: 日本育種学会第120回講演会秋季大会
  • Place of Presentation: 福井県立大学（福井市）
• [Presentation] いもち病菌非病原力遺伝子AVR-Pikとイネいもち病抵抗性遺伝子Pikは、直接的タンパク質相互作用によって認識する
  • Author(s): 神崎 洋之、吉田 健太郎、平渕亜紀子、寺内 良平
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場（福岡市）
• [Book] Protoplast cell death assay to study AVR function in rice (in Methods in Molecular Biology ; Plant Immunity) (2014)
  • Author(s): Hiroyuki Kanzaki, Kentaro Yoshida, Hiromasa, Saitoh, Muluneh Tamiru and Ryohei Terauchi (Edit. Baul Birch)
  • Publisher: Springer
• [Book] Plant Immunity (Springer)DOI : 10.1007/978-1-62703-986-4_20 (2013)
  • Author(s): Kanzaki. H., Yoshida, K., Saitoh, H., Tamiru, M. Terauchi, R. (Edit. Baul Birch)
  • Publisher: Protoplast cell death assay to study AVR function in rice (in Methods in Molecular Biology
• [Book] Protoplast cell death assay to study AVR function in rice (in Methods in Molecular Biology ; Plant Immunity) (2013)
  • Author(s): Hiroyuki Kanzaki, Kentaro Yoshida, Hiromasa, Saitoh, Muluneh Tamiru and Ryohei Terauchi (Edit. Baul Birch)
  • Publisher: Springer