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Characterization of antenna-specific cholesterol-binding protein in the ant

Research Project

Project/Area Number 23580070
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied entomology
Research InstitutionToyama Prefectural University (2012-2013)
Kobe University (2011)

Principal Investigator

ISHIDA Yuko  富山県立大学, その他部局等, 研究員 (90509861)

Co-Investigator(Kenkyū-buntansha) YAMAZAKI Toshimasa  独立法人農業生物資源研究所, 農業生物先端ゲノム研究センター, ユニット長 (40360458)
MIYAZAWA Mitsuhiro  独立法人農業生物資源研究所, 農業生物先端ゲノム研究センター, ユニット長 (90370684)
Co-Investigator(Renkei-kenkyūsha) ISHIBASHI Jun  独立法人農業生物資源研究所, 農業生物先端ゲノム研究センター, 主任研究員 (20391576)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsニーマンピック2型タンパク質 / クロオオアリワーカー / 蛍光拮抗アッセイ / 円偏光二色性 / GC-EAD / 結晶化学 / 情報伝達物質 / Niemann-Pick Type C2 / 円偏光偏光二色性 / クロオオアリ / Niemann-Pick Type C-2 / 組織局在性 / 塩基配列決定 / 組換えタンパク質 / 大量精製
Research Abstract

We identified Niemann-Pick type C2 protein antenna-specifically expressed in worker of the Japanese carpenter ant, Camponotus japonicus. In human, this protein plays an essential role in transferring cholesterol in the cells whereas ant accumulated this protein in the basiconic sensillum and used it as a carrier protein for semiochemicals such as long-chain fatty acids, alcohols, and acetates. The protein was composed of beta-structure. The molecular mechanism of binding release was regulated by hydrogen network formation and angle change of side chain of the 112th tryptophan residue.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (28 results)

All 2014 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 7 results) Presentation (8 results) Remarks (9 results)

  • [Journal Article] Niemann-Pick type C2 protein mediating chemical communication in the worker ant2014

    • Author(s)
      Ishida, Y., Tsuchiya, W., Fujii, T., Fujimoto, Z., Miyazawa, M., Ishibashi, J., Matsuyama, S., Ishikawa, Y., and Yamazaki, T
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 111 Issue: 10 Pages: 3847-3852

    • DOI

      10.1073/pnas.1323928111

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Fatty acid solubilizer from the oral disk of the blowfly2013

    • Author(s)
      Ishida, Y., Ishibashi, J., and Leal, W. S
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 1 Pages: e51779-e51779

    • DOI

      10.1371/journal.pone.0051779

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Crystallographic observation of pH-Induced conformational changes in the Amyelois transitella pheromone-binding protein AtraPBP12013

    • Author(s)
      Eric di Luccio, Yuko Ishida, Walter S. Leal, and David K. Wilson
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 2 Pages: e53840-e53840

    • DOI

      10.1371/journal.pone.0053840

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] カイコ蛾フェロモン結合タンパク質のフォールディングと動的挙動2012

    • Author(s)
      宮澤光博、石田裕幸、Leal, W. S
    • Journal Title

      蚕糸・昆虫バイオテック

      Volume: 81 Pages: 131-137

    • NAID

      130004491452

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] 昆虫の嗅覚・味覚受容に与る感覚子の機能と水の関係2012

    • Author(s)
      石田裕幸
    • Journal Title

      蚕糸・昆虫バイオテック

      Volume: 81 Pages: 23-30

    • NAID

      10031045815

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] A water-specific aquaporin is expressed in the olfactory organs of the blowfly, Phormia regina2012

    • Author(s)
      Ishida, Y., Nagae, T., and Azuma, M.
    • Journal Title

      J. Chem. Ecol

      Volume: 38 Issue: 8 Pages: 1057-1061

    • DOI

      10.1007/s10886-012-0157-z

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aversive odorant causing appetite decrease downregulates tyrosine decarboxylase gene expression in the olfactory receptor neuron of the blowfly, Phormia regina2012

    • Author(s)
      Yuko Ishida, and Mamiko Ozaki
    • Journal Title

      Naturwissenscheaften

      Volume: 99 Issue: 1 Pages: 71-75

    • DOI

      10.1007/s00114-011-0865-1

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Starvation-induced elevation of taste responsiveness and expression of a sugar taste receptor gene in Drosophila melanogaster.2012

    • Author(s)
      Nishimura, A., Ishida, Y., Takahashi, A., Okamoto, H., Sakabe, M., Itoh, M., Takano-Shimizu, T., and Ozaki M.
    • Journal Title

      Journal of Neurogenetics

      Volume: 26 Issue: 2 Pages: 206-215

    • DOI

      10.3109/01677063.2012.694931

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Methyl-beta-cyclodextrin is a useful compound for extraction and purification of prenylated enzymes from the retinal disc membrane2012

    • Author(s)
      Natsumi Saito, Yuko Ishida, Keiji Seno, and Fumio Hayashi
    • Journal Title

      Protein Expression and Purification

      Volume: 82 Issue: 1 Pages: 168-173

    • DOI

      10.1016/j.pep.2011.12.007

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A putative octopamine/tyramine receptor mediating appetite in a hungry fly2011

    • Author(s)
      Ishida, Y., and Ozaki, M.
    • Journal Title

      Naturwissenschaften

      Volume: 98 Pages: 635-638

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dynamic conformational equilibria in the physiological function of the Bombyx mori pheromone-binding protein2011

    • Author(s)
      Michel, E., Damberger, F., F., Ishida, Y., Fiorito, F., Lee, D., Leal, W. S., and Wüthrich, W.
    • Journal Title

      J. Mol. Biol.

      Volume: 408 Pages: 922-931

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] クロキンバエ口盤特異的に蓄積する脂肪酸可溶化タンパク質の同定、局在性2013

    • Author(s)
      石田裕幸・石橋純・Walter S. Leal
    • Organizer
      平成25年度蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      農林水産技術会議事務局筑波事務所本館
    • Related Report
      2013 Final Research Report
  • [Presentation] クロキンバエ口盤特異的に蓄積する脂肪酸可溶化タンパク質の同定、局在性、機能解析2013

    • Author(s)
      石田裕幸・石橋純・Walter S. Leal
    • Organizer
      日本蚕糸学会
    • Place of Presentation
      農林水産技術会議事務局筑波事務所
    • Related Report
      2012 Research-status Report
  • [Presentation] クロキンバエの匂い受容器官で発現するアクアポリンの同定、局在性クロキンバエにおける忌避匂い物質による食欲減退機構:チラミン生合成酵素、代謝酵素、受容体遺伝子の発現と局在性2012

    • Author(s)
      石田裕幸・永江知音
    • Organizer
      東政明2012年日本蚕糸学会合同支部大会
    • Place of Presentation
      信州大学繊維学部
    • Related Report
      2013 Final Research Report
  • [Presentation] クロキンバエにおける忌避匂い物質による食欲減退機構:チラミン生合成酵素、代謝酵素、受容体遺伝子の発現と局在性2012

    • Author(s)
      石田裕幸・尾崎まみこ
    • Organizer
      第56回日本応用動物昆虫学会大会
    • Place of Presentation
      近畿大学奈良キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] クロオオアリワーカー触角特異的ヒトコレステロール結合タンパク質類似タンパク質の同定、発現、性状2012

    • Author(s)
      石田裕幸・土屋渉・石橋純・宮澤光博・山崎俊正
    • Organizer
      平成24年度蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      九州大学箱崎キャンパス文系地区
    • Related Report
      2013 Final Research Report
  • [Presentation] クロキンバエの匂い受容器官で発現するアクアポリンの同定、局在性、機能解析2012

    • Author(s)
      石田裕幸・永江知音・東政明
    • Organizer
      日本蚕糸学会
    • Place of Presentation
      信州大学繊維学部
    • Related Report
      2012 Research-status Report
  • [Presentation] クロオオアリワーカー触角特異的ヒトコレステロール結合タンパク質類似タンパク質の同定、発現、性状解析2012

    • Author(s)
      石田裕幸・土屋渉・石橋純・宮澤光博・山崎俊正
    • Organizer
      日本蚕糸学会平成24年度蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      九州大学箱崎キャンパス文系地区
    • Related Report
      2011 Research-status Report
  • [Presentation] クロキンバエにおける忌避匂い物質による食欲減退機構:チラミン生合成酵素、代謝酵素、受容体遺伝子の発現と局在性2012

    • Author(s)
      石田裕幸・尾崎まみこ
    • Organizer
      第56回日本応用動物昆虫学会大会
    • Place of Presentation
      近畿大学農学部
    • Related Report
      2011 Research-status Report
  • [Remarks] 富山県立大学

    • URL

      http://www.pref.toyama.jp/cms_press/2014/20140226/00013949.pdf

    • Related Report
      2013 Final Research Report
  • [Remarks] 農業生物資源研究所

    • URL

      http://www.nias.affrc.go.jp/press/2014/20140224/index.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 東京大学

    • URL

      https://sites.google.com/a/utlae.org/jp/home/announcements/fushanxianlidaxue%E3%80%81shengwuyan%E3%80%81zhubodatonogongtongyanjiugapnasnijiezai

    • Related Report
      2013 Final Research Report
  • [Remarks] 共同通信PRワイヤー、CNET Japan、産経新聞、中日新聞、農業協同組合新聞、日本農業新聞、化学工業日報、北日本新聞、富山新聞など様々なメディアで本研究成果(雑誌論文1)が紹介された。

    • Related Report
      2013 Final Research Report
  • [Remarks] 講演, クロオオアリワーカー触角特異的情報伝達物質運搬タンパク質の同定、機能解析、構造決定

    • Related Report
      2013 Final Research Report
  • [Remarks] 石田裕幸, 名古屋大学大学院生命農学研究科20140328

    • Related Report
      2013 Final Research Report
  • [Remarks] アリのコロニー ( 集団 ) の維持に不可欠な情報伝達物質を運搬する 新規のタンパク質を発見

    • URL

      http://www.pu-toyama.ac.jp/wordpress/wp-content/uploads/2014/02/26-2-26ishida.pdf

    • Related Report
      2013 Annual Research Report
  • [Remarks] アリの情報交換に関わる新型タンパク質を発見

    • URL

      http://www.nias.affrc.go.jp/press/2014/20140224/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 富山県立大学、生物研、筑波大との共同研究がPNASに掲載

    • URL

      https://sites.google.com/a/utlae.org/jp/home/announcements/fushanxianlidaxue%E3%80%81shengwuyan%E3%80%81zhubodatonogongtongyanjiugapnasnijiezai

    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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