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Resistance mechanisms of the diamondback moth against various insecticides

Research Project

Project/Area Number 23580072
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied entomology
Research InstitutionOkayama University

Principal Investigator

SONODA Shoji  岡山大学, 資源植物科学研究所, 准教授 (00325127)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsアセチルコリンエステラーゼ / コナガ / 遺伝子重複 / アミノ酸変異 / 有機リン剤 / 殺虫剤抵抗性 / RNA editing / RNA variegation / ピレスロイド剤 / ナトリウムチャネル
Research Abstract

This study examined the acetylcholinesterase 1 gene (AChE1) in Plutella xylostella strains with different sensitivities to acephate. Multiple haplotypes of the gene were found in the field-collected strains including distinct haplotypes carrying one or both previously reported mutations (A298S and G324A). Moreover, sequencing results indicated the presence of duplicated copies of the gene in the fieldcollected strains. No correlation was found between copy numbers of AChE1 and levels of resistance to acephate suggesting that extensive AChE1 duplication is not a major resistance factor at least in some P. xylostella strains. Proportions of the A298S and G324A mutations showed no correlation with levels of resistance to acephate. This suggests that acephate resistance of P. xylostella is complex and cannot be evaluated based on the AChE1 copy number or proportions of the resistance mutations alone.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (21 results)

All 2014 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (15 results) (of which Invited: 3 results) Book (1 results)

  • [Journal Article] Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate2014

    • Author(s)
      Sonoda, S., Shi, X., Song, D., Liang, P., Gao, X., Zhang, Y., Li, J., Liu, Y., Li, M., Matsumura, M., Sanada-Morimura, S., Minakuchi, C., Tanaka, T., Miyata, T
    • Journal Title

      Insect Biochem. Mol. Biol

      Volume: 48 Pages: 83-90

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate2014

    • Author(s)
      Shoji Sonodaa, Xueyan Shib, Dunlun Songb, Pei Liangb, Xiwu Gaob, Youjun Zhangc, Jianhong Lid, Yong Liue, Ming Lif, Masaya Matsumurag, Sachiyo Sanadag, Chieka Minakuchih, Toshiharu Tanakah, Tadashi Miyata
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: 48 Pages: 83-90

    • DOI

      10.1016/j.ibmb.2014.02.008

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Frequencies of the M918I mutation in the sodium channel of the diamondback moth in China, Thailand and Japan and its association with pyrethroid resistance2012

    • Author(s)
      Sonoda, S., Shi, X., Song, D., Zhang, Y., Li J., Wu, G., Liu, Y., Li, M., Liang, P., Wari, D., Matsumura, M., Minakuchi, C., Tanaka, T., Miyata, T., Gao, X
    • Journal Title

      Pestic. Biochem. Physiol.

      Volume: 102 Pages: 102-105

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Frequeincies of the M918I mutation in the sodium channel of the diamondback moth in China, Thailand and Japan and its association with pyrethroid resistance2012

    • Author(s)
      Sonoda, S. et al.
    • Journal Title

      Pesticide Biochemistry and Physiology

      Volume: 102 Issue: 2 Pages: 142-145

    • DOI

      10.1016/j.pestbp.2011.12.005

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 殺虫剤抵抗性機構の解析と今後の課題2012

    • Author(s)
      園田昌司
    • Journal Title

      植物防疫

      Volume: 66 Pages: 40-45

    • NAID

      40019213154

    • Related Report
      2011 Research-status Report
  • [Presentation] コナガにおけるAChE遺伝子の重複と有機リン剤抵抗性2014

    • Author(s)
      園田昌司
    • Organizer
      第58回日本応用動物昆虫学会大会
    • Place of Presentation
      高知大学
    • Year and Date
      2014-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 殺虫剤の作用機作と抵抗性のメカニズム2013

    • Author(s)
      園田昌司
    • Organizer
      長野県病害虫防除シンポジウム
    • Place of Presentation
      松本市
    • Year and Date
      2013-02-22
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] コナガのピレスロイド剤抵抗性-野外におけるナトリウムチャネルのアミノ酸変異2012

    • Author(s)
      園田昌司
    • Organizer
      NIASシンポジウム「ポストゲノム時代の害虫防除研究のあり方」,第5回-殺虫剤抵抗性問題の最前線
    • Place of Presentation
      東京都
    • Year and Date
      2012-11-15
    • Related Report
      2013 Final Research Report
  • [Presentation] 難防除害虫の薬剤抵抗性メカニズム2012

    • Author(s)
      園田昌司
    • Organizer
      革新的農業技術習得支援事業(野菜の難防除害虫のIPM技術)
    • Place of Presentation
      津市
    • Year and Date
      2012-10-25
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] コナガの殺虫剤に対する抵抗性および適応機構2012

    • Author(s)
      園田昌司
    • Organizer
      九州昆虫セミナー
    • Place of Presentation
      合志市
    • Year and Date
      2012-10-22
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Organophosphate resistance in the laboratory and field strains of the diamondback moth2012

    • Author(s)
      Sonoda, S
    • Organizer
      International Seminar on the Development of Insecticide Resistance and Its Management in the Diamondback Moth
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2012-08-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Frequencies of the M918I, T929I and L1014F mutations in the sodium channel of the diamondback moth in China, Thailand and Japan2012

    • Author(s)
      Sonoda, S, et al.
    • Organizer
      XXIV International Congress of Entomology
    • Place of Presentation
      Daegu, Korea
    • Year and Date
      2012-08-20
    • Related Report
      2013 Final Research Report
  • [Presentation] コナガの合成ピレスロイド剤抵抗性に関わるナトリウムチャネルのアミノ酸変異の頻度-日本、中国、タイ系統の比較2012

    • Author(s)
      園田昌司, et al
    • Organizer
      日本農薬学会大会第37回大
    • Place of Presentation
      岡山大学
    • Year and Date
      2012-03-15
    • Related Report
      2013 Final Research Report
  • [Presentation] コナガの合成ピレスロイド剤抵抗性に関わるナトリウムチャネルのアミノ酸変異の頻度 -日本、中国、タイ系統の比較-2012

    • Author(s)
      園田昌司
    • Organizer
      日本農薬学会大会第37回大会
    • Place of Presentation
      岡山大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Sodium channel mutations associated with pyrethroid resistance and their frequencies in the field strains of the diamondback moth, Plutella xylostella collected at different Asian countries2011

    • Author(s)
      Sonoda, S
    • Organizer
      International Symposium on Insecticide Resistance Management
    • Place of Presentation
      Guiyamg, China
    • Year and Date
      2011-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Sodium channel mutations associated with pyrethroid resistance and their frequencies in the field strains of the diamondback mth, Plutella xylostella collected at different Asian countries.2011

    • Author(s)
      Sonoda, S.
    • Organizer
      International Symposium on Insecticide Resistance Management(招待講演)
    • Place of Presentation
      Guiyamg, China
    • Related Report
      2011 Research-status Report
  • [Presentation] コナガにおけるAChE1遺伝子の重複と有機リン剤抵抗性

    • Author(s)
      園田昌司
    • Organizer
      第58回日本応用動物昆虫学会大会
    • Place of Presentation
      高知大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Frequencies of the M918I, T929I and L1014F mutations in the sodium channel of the diamondback moth in China, Thailand and Japan

    • Author(s)
      Sonoda, S. et al.
    • Organizer
      XXIV International Congress of Entomology
    • Place of Presentation
      Daegu, Korea
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Organophosphate resistance in the laboratory and field strains of the diamondback moth

    • Author(s)
      Sonoda, S.
    • Organizer
      International Seminar on the Development of Insecticide Resistance and Its Management in the Diamondback Moth
    • Place of Presentation
      Nagoya, Japan
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] コナガのピレスロイド剤抵抗性-野外におけるナトリウムチャネルのアミノ酸変異-

    • Author(s)
      園田昌司
    • Organizer
      NIASシンポジウム「ポストゲノム時代の害虫防除研究のあり方」第5回-殺虫剤抵抗性問題の最前線-
    • Place of Presentation
      東京都
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 植物防疫, 殺虫剤抵抗性機構の解析と今後の課題2012

    • Author(s)
      園田昌司
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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