Resistance mechanisms of the diamondback moth against various insecticides
Project/Area Number |
23580072
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied entomology
|
Research Institution | Okayama University |
Principal Investigator |
SONODA Shoji 岡山大学, 資源植物科学研究所, 准教授 (00325127)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | アセチルコリンエステラーゼ / コナガ / 遺伝子重複 / アミノ酸変異 / 有機リン剤 / 殺虫剤抵抗性 / RNA editing / RNA variegation / ピレスロイド剤 / ナトリウムチャネル |
Research Abstract |
This study examined the acetylcholinesterase 1 gene (AChE1) in Plutella xylostella strains with different sensitivities to acephate. Multiple haplotypes of the gene were found in the field-collected strains including distinct haplotypes carrying one or both previously reported mutations (A298S and G324A). Moreover, sequencing results indicated the presence of duplicated copies of the gene in the fieldcollected strains. No correlation was found between copy numbers of AChE1 and levels of resistance to acephate suggesting that extensive AChE1 duplication is not a major resistance factor at least in some P. xylostella strains. Proportions of the A298S and G324A mutations showed no correlation with levels of resistance to acephate. This suggests that acephate resistance of P. xylostella is complex and cannot be evaluated based on the AChE1 copy number or proportions of the resistance mutations alone.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate2014
Author(s)
Sonoda, S., Shi, X., Song, D., Liang, P., Gao, X., Zhang, Y., Li, J., Liu, Y., Li, M., Matsumura, M., Sanada-Morimura, S., Minakuchi, C., Tanaka, T., Miyata, T
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Journal Title
Insect Biochem. Mol. Biol
Volume: 48
Pages: 83-90
Related Report
Peer Reviewed
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[Journal Article] Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate2014
Author(s)
Shoji Sonodaa, Xueyan Shib, Dunlun Songb, Pei Liangb, Xiwu Gaob, Youjun Zhangc, Jianhong Lid, Yong Liue, Ming Lif, Masaya Matsumurag, Sachiyo Sanadag, Chieka Minakuchih, Toshiharu Tanakah, Tadashi Miyata
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Journal Title
Insect Biochemistry and Molecular Biology
Volume: 48
Pages: 83-90
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Frequencies of the M918I mutation in the sodium channel of the diamondback moth in China, Thailand and Japan and its association with pyrethroid resistance2012
Author(s)
Sonoda, S., Shi, X., Song, D., Zhang, Y., Li J., Wu, G., Liu, Y., Li, M., Liang, P., Wari, D., Matsumura, M., Minakuchi, C., Tanaka, T., Miyata, T., Gao, X
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Journal Title
Pestic. Biochem. Physiol.
Volume: 102
Pages: 102-105
Related Report
Peer Reviewed
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