| Project/Area Number |
23580074
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MASEDA Hideaki 徳島大学, ソシオテクノサイエンス研究部, 准教授 (10372343)
OIKAWA Akira 山形大学, 農学部, 准教授 (50442934)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | aphid / rice / amino acid / tryptophan / serotonin / Rhopalosiphum / rufiabdominale / イネ / アブラムシ / 誘導抵抗性 / セロトニン / 成長阻害 / アミノ酸 / オカボノアカアブラムシ / ﾄﾘﾌﾟﾄﾌｧﾝ / 褐変 / ペルオキシダーゼ / 抵抗性 / 防御機構 / メタボローム |
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| Outline of Final Research Achievements |
Asparagine and Glutamine, which were important nutritions for Buchnera, were selectively accumulated in a root of rice plant seedling which was infested with aphids (Rhopalosiphum rufiabdominale). The change of transcription level was observed in 20-30% of amino acid biosynthetic pathway genes, when a root was infested with aphids. So it was presumed that this change result in the selective accumulation of amino acids. Actually, the transcription of biosynthetic genes, Glutamate synthase, Asparagine synthase, and Tryptophan synthase, was increase to 2.4, 3.0, and 17.1-fold, respectively, at 5 day after infection of aphids. On the other hand the transcription of Tryptophan decarboxylase, which could promote conversion from tryptophan to tryptamine, was increase to 32-fold. Thus a modification of a flux balance on metabolism pathway and/or a change of main pathway were occurred. So it was thought that these changes would lead to the selective accumulation of the amino acids.
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