Study on insect disease caused by Serratia

• Project/Area Number: 23580075
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Kyushu University
• Principal Investigator: IIYAMA Kazuhiro 九州大学, (連合)農学研究科(研究院), 助教 (70325489)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: Serratia lquefaciens / 病原力因子 / 病原性 / リポ多糖 / Serratia liquefaciens / Serratia liquefacines / カイコ / トランスポゾン / 昆虫病原性細菌 / セラチア / プロテアーゼ / 病原力 / 組換えタンパク質

Outline of Final Research Achievements

In order to study pathogenesis of Serratia liquefaciens to insect, transposon library was constructed. Among 1200 transposon mutants, four mutant clearly decreased virulence to the silkworm, Bombyx mori. Next, whole genome of S. liquefaciens was sequenced by next generation sequence technology. Single transposon was inserted into lipopolysaccharide biosynthetic gene in the four mutants. The gene complemented strains were restored its virulence. These results indicated that lipopolysaccharides of S. liquefaciens has an important role in its pathogenesis to insect.

Research Products

• [Journal Article] Draft genome sequence of entomopathogenic Serratia liquefaciens strain FK01. (2014)
  • Author(s): Erika Taira, Kazuhiro Iiyama, Hiroaki Mon, Kazuki Mori, Taiki Akasaka, Kousuke Tashiro, Chisa Yasunaga-Aoki, Jae Man Lee, Takahiro Kusakabe
  • Journal Title: Genome announcements Volume: 2 Issue: 3
  • DOI: 10.1128/genomea.00609-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] プラスミドpNKBORmobを用いたSerratia liquefaciensの効率的なトランスポゾン変異株の作出 (2014)
  • Author(s): 平 詠里加・大垰勝寛・飯山和弘・青木智佐・李 在萬・日下部宜宏
  • Journal Title: Entomotech Volume: 38 Pages: 17-19
  • Peer Reviewed
• [Journal Article] Construction of serralysin-like metalloprotease- deficient mutants of Serratia liquefaciens and their virulence in the silkworm, Bombyx mori. (2013)
  • Author(s): Fusako Kaibara, Kazuhiro Iiyama, Yuuka Chieda, Jae Man Lee, Takahiro Kusakabe, Chisa Yasunaga-Aoki and Susumu Shimizu
  • Journal Title: Journal of Insect Biotechnology and Sericology Volume: 印刷中
  • NAID: 10031163349
  • Peer Reviewed
• [Journal Article] Improvement in pHERD vectors to express recombinant proteins tagged with hexahistidine at either the NH2- or COOH- terminal in Pseudomonas aeruginosa. (2011)
  • Author(s): Iiyama, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S.
  • Journal Title: J. Insect Biotechnol. Sericol. Volume: 80 Pages: 57-61
  • NAID: 130004464181
  • Peer Reviewed
• [Presentation] Virulence of Serratia liquefaciens against Bomyx mori. (2014)
  • Author(s): Erika Taira, Kazuhiro Iiyama, Jae Man Lee, Takahiro Kusakabe, Chisa Yasunaga-Aoki
  • Organizer: International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2014 (The 11th International Joint Symposium between Japan and Korea)
  • Place of Presentation: Ladena Condominium in Chuncheon, Korea
  • Year and Date: 2014-10-29 – 2014-10-30
• [Presentation] Serratia liquefaciens のリポ多糖生合成変異株はカイコに対する病原力が低下する (2014)
  • Author(s): 平 詠里加・飯山和弘・李 在萬・日下部宜宏・青木智佐
  • Organizer: 第11回昆虫病理研究会シンポジウム
  • Place of Presentation: 富士Calm 山梨
  • Year and Date: 2014-09-18 – 2014-09-20
• [Presentation] In vivo スクリーニングによるSerratia liquefaciens のカイコに対する病原力因子の探索（２） (2014)
  • Author(s): 平 詠里加・大垰勝寛・飯山和弘・青木智佐・李 在萬・日下部宜宏
  • Organizer: 平成26年度 蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 日本大学生物資源科学部 神奈川
• [Presentation] In vivo スクリーニングによるSerratia liquefaciens のカイコに対する病原力因子の探索 (2013)
  • Author(s): 平 詠里加・飯山和弘・青木智佐・李 在萬・日下部宜宏
  • Organizer: 平成25年度第69回日本蚕糸学会九州支部研究発表会 昆虫機能・利用学術講演会
  • Place of Presentation: 久米島博物館 沖縄
• [Presentation] Overexpression and purification of the recombinant serralysin-like metalloproteases in Escherichia coli. (2012)
  • Author(s): Kosuke Kawano, Kazuhiro Iiyama, Jae Man Lee, Takahiro Kusakabe, Chisa Yasunaga-Aoki, Susumu Shimizu
  • Organizer: ICE2012 XXIV International Congress of Entomology
  • Place of Presentation: Daegu, Korea
• [Presentation] 大腸菌発現系を用いた組換えセラリシン様メタロプロテアーゼの発現および精製 (2) (2012)
  • Author(s): 河野康亮・飯山和弘・李 在萬・日下部宜宏・青木智佐・清水 進
  • Organizer: 第10回昆虫病理研究会シンポジウム
  • Place of Presentation: 帯広畜産大学 北海道
• [Presentation] 大腸菌発現系を用いた組換えセラリシン様メタロプロテアーゼの発現および精製 (2012)
  • Author(s): 河野康亮・飯山和弘・李 在萬・日下部宜宏・青木智佐・清水 進
  • Organizer: 平成24年度 蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 九州大学箱崎キャンパス文系地区 福岡
• [Presentation] 組換えセラリシン様メタロプロテアーゼの発現，精製の試み (2011)
  • Author(s): 河野康亮・飯山和弘・李 在萬・日下部宜宏・青木智佐・清水 進
  • Organizer: 本蚕糸学会第65回東北支部・第62回関東支部・第77回関西支部・第67回九州支部合同大会 昆虫機能・利用学術講演会
  • Place of Presentation: 岩手大学農学部 岩手