Analysis of dsRNA mechanism of response in insect cell
| Project/Area Number |
23580077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Kyushu University |
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Principal Investigator |
LEE JAEMAN 九州大学, (連合)農学研究科(研究院), 助教 (50404083)
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| Co-Investigator(Kenkyū-buntansha) |
日下部 宜宏 九州大学, 大学院農学研究院, 教授 (30253595)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | RNA interference / 遺伝子機能阻害 / dsRNA応答機構 / BmAgo1 / BmAgo2 / BmMLV / RISC因子 / RNAi / RNAi誘導実験 / siRNA / miRNA / Soaking RNAi誘導 / 遺伝子機能解析 / sid-1 / BmN4細胞 |
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| Research Abstract |
In order to establish efficient RNA interference induction system in insect cells, we introduced several membrane proteins, which have the ability to uptake dsRNA into the cells. Also the cellular responses against dsRNA uptake were analyzed using silkworm cells. As a result, we found that C. elegans Sid-1 membrane protein possessed an ability to uptake dsRNA efficiently. Using this protein, several transgenic insect cell lines were successfully established. Furthermore, almost all RNAi related components of silkworm were isolated and characterized.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathway in the silkworm2013
Author(s)
Li Zhu, Zhiqing Li, Tsuneyuki Tatsuke, Daojun Cheng, Jian Xu, Kaito Yoshimura, Hiroaki Mon, Kazuhiro Iiyama, JaeMan Lee, Qingyou Xia, Takahiro Kusakabe
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Journal Title
J. Insect Biotechnol. Seric
Volume: 82
Pages: 19-23
Related Report
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[Journal Article] Coexpression of Escherichia coli RNase III in silkworm cells improves the efficiency of RNA interference induced by long hairpin dsRNAs2013
Author(s)
Lee, J.M., Kojin, Y., Tatsuke, T., Mon, H., Miyagawa, Y. and Kusakabe, T.
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Journal Title
Insect Science
Volume: 20
Issue: 1
Pages: 69-77
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular cloning of BmTUDOR-SN and analysis of its role in the RNAi pathway in the silkworm, Bombyx mori (Lepidoptera: Bombycidae)2012
Author(s)
Zhu, L., Tatsuke, T., Li, Z., Mon, H., Xu, J., Lee, J.M. and Kusakabe, T.
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Journal Title
Applied entomology and zoology
Volume: 47
Pages: 207-215
Related Report
Peer Reviewed
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[Journal Article] RNA Interference Induction by Long Hairpin dsRNAs Expressed from Chromosomal DNA of Bombyx mori Cells2012
Author(s)
Lee, J.M., Kojin, Y., Tatsuke, T., Mon, H., Miyagawa, Y. and Kusakabe, T.
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Journal Title
Journal of the Faculty of Agriculture, Kyushu University
Volume: 57
Pages: 441-445
NAID
Related Report
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