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Epigenetics of insect polymorphism

Research Project

Project/Area Number 23580079
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied entomology
Research InstitutionTamagawa University

Principal Investigator

SASAKI Tetsuhiko  玉川大学, 学術研究所, 教授 (60235257)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsエピジェネティックス / ミツバチ / アブラムシ / 多型発現 / カースト分化 / エピジェネティクス
Research Abstract

Many insects exhibit polymorphism, producing different forms from the same genome. Such examples include queen and worker caste differentiation in honeybees and development of alate (winged) and apterous (non-winged) morphs in aphids. To investigate the epigenetic mechanisms underlying the occurrence of polymorphism, genome-wide methylation profiles were analyzed in the European honeybee, Apis mellifera, and in the pea aphids, Acyrthosiphon pisum. In the honeybee, 1,717 CpG sites were found to be differently methylated between the two castes. Interestingly, differently methylated genes included several sex-determining genes, implying that genes for sex determination might be also involved in caste differentiation. In the aphids, 226 were differently methylated between the two morphs.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (18 results)

All 2014 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (11 results) Remarks (2 results)

  • [Journal Article] Quantitative analysis of the lytic cycle of WO phages infecting Wolbachia.2012

    • Author(s)
      Furukawa S., Tanaka K., Ikeda T., Fukatsu T. and Sasaki T
    • Journal Title

      Applied Entomology and Zoology

      Volume: 47 Issue: 4 Pages: 449-456

    • DOI

      10.1007/s13355-012-0142-6

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] CpG methylation in the hexamerin 110 gene in the European honeybee Apis mellifera L.2011

    • Author(s)
      Ikeda T., Furukawa S., Nakamura J., Sasaki M. and Sasaki T
    • Journal Title

      J. Insect Science

      Volume: 11 Pages: 74-74

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transduction of baculovirus vectors to queen honeybees, Apis mellifera L2011

    • Author(s)
      Ikeda T., Nakamura J., Furukawa S., Chan- tawannakul P., Sasaki M. and Sasaki T
    • Journal Title

      Apidologie

      Volume: 42 Issue: 4 Pages: 461-471

    • DOI

      10.1007/s13592-011-0014-z

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transduction of baculovirus vectors to queen honeybees, Apis mellifera L.2011

    • Author(s)
      Ikeda T. et al.
    • Journal Title

      Apidologie

      Volume: 42 Pages: 461-471

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] CpG methylation in the hexamerin 110 gene in the European honeybee Apis mellifera L.2011

    • Author(s)
      Ikeda T. et al.
    • Journal Title

      J. Insect Science

      Volume: 11

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] セイヨウミツバチ働きバチの脳におけるDNAメチル化解析2014

    • Author(s)
      坂本洋典、緒方法親、佐々木哲彦
    • Organizer
      日本応用動物昆虫学会第58回大会
    • Place of Presentation
      高知大学,高知市
    • Related Report
      2013 Final Research Report
  • [Presentation] セイヨウミツバチ働きバチの脳におけるDNAメチル化解析2014

    • Author(s)
      坂本洋典、緒方法親、佐々木哲彦
    • Organizer
      日本応用動物昆虫学会第58回大会
    • Place of Presentation
      高知大学(高知県、高知市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Comparison of genome-wide DNA methylation pattern between queen larvae and worker larvae in the European honeybees2013

    • Author(s)
      Sakamoto H., Suzuki M. and Sasaki T
    • Organizer
      The 2nd Grobal Conference on Entomology
    • Place of Presentation
      Kuching, Malaysia
    • Related Report
      2013 Final Research Report
  • [Presentation] セイヨウミツバチの女王と働きバチのゲノムメチル化の比較2013

    • Author(s)
      坂本洋典、鈴木美穂、佐々木哲彦
    • Organizer
      第三回NGS(Next Generation Sequence)現場の会
    • Place of Presentation
      神戸国際会議場,神戸市
    • Related Report
      2013 Final Research Report
  • [Presentation] セイヨウミツバチのカースト間におけるゲノムメチル化の比較2013

    • Author(s)
      坂本洋典、鈴木美穂、佐々木哲彦
    • Organizer
      日本応用動物昆虫学会第57回大会
    • Place of Presentation
      日本大学、藤沢市
    • Related Report
      2013 Final Research Report
  • [Presentation] Comparison of genome-wide DNA methylation pattern between queen larvae and worker larvae in the European honeybees.2013

    • Author(s)
      Sakamoto H., Suzuki M. and Sasaki T.
    • Organizer
      The 2nd Grobal Conference on Entomology
    • Place of Presentation
      Kuching、 Malaysia
    • Related Report
      2013 Annual Research Report
  • [Presentation] セイヨウミツバチの女王と働きバチのゲノムメチル化の比較2013

    • Author(s)
      坂本洋典、鈴木美穂、佐々木哲彦
    • Organizer
      第三回NGS(Next Generation Sequence)現場の会
    • Place of Presentation
      神戸国際会議場(兵庫県、神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] セイヨウミツバチのカースト間におけるゲノムメチル化の比較2013

    • Author(s)
      坂本洋典、鈴木美穂、佐々木哲彦
    • Organizer
      日本応用動物昆虫学会第57回大会
    • Place of Presentation
      日本大学、神奈川県
    • Related Report
      2012 Research-status Report
  • [Presentation] DNA methylation during the caste determination in the European honeybees, Apis mellifera2012

    • Author(s)
      Sakamoto H., Suzuki M. and Sasaki T
    • Organizer
      The 24th International Congress of Entomology
    • Place of Presentation
      Daegu, Korea
    • Related Report
      2013 Final Research Report
  • [Presentation] セイヨウミツバチのカースト決定期におけるDNAメチル化の解析2012

    • Author(s)
      石井進之輔、佐々木哲彦、鈴木美穂
    • Organizer
      日本応用動物昆虫学会第56回大会
    • Place of Presentation
      近畿大学、奈良市
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] DNA methylation during the caste determination in the European honeybees, Apis mellifera.2012

    • Author(s)
      Sakamoto H., Suzuki M. and Sasaki T.
    • Organizer
      The 24th International Congress of Entomology
    • Place of Presentation
      Daegu, Korea
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://libds.tamagawa.ac.jp/dspace/bitstream/11078/97/1/7_04.pdf

    • Related Report
      2013 Final Research Report
  • [Remarks] 玉川大学グローバルCOE事業推進担当者

    • URL

      http://gcoe.tamagawa.ac.jp/jpn/member/detail.html?id=14

    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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