Genetic and endocrine control of insect hypermetamorphosis

• Project/Area Number: 23580080
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Minami Kyusyu University
• Principal Investigator: SHINTANI Yoshinori 南九州大学, 環境園芸学部, 教授 (50389574)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Seiji 国立研究開発法人農業生物資源研究所, 昆虫科学研究領域昆虫-昆虫・植物間相互作用研究ユニット, 上級研究員 (50370664) ; SHINODA Tetsuro 国立研究開発法人農業生物資源研究所, 昆虫科学研究領域制御剤標的遺伝子研究ユニット, ユニット長 (10355620)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 過変態 / 遺伝子発現 / 内分泌機構 / マメハンミョウ / 擬蛹 / 休眠 / 表現型可塑性 / 次世代シークエンス / 過変態昆虫 / 内分泌 / Epicauta gorhami / hypermetamorphosis / phenotypic plasticity / pseudopupa / diapause / photoperiodism / RNA / hypermetamorohsis / molecular mechanism / hormonal control / gene expression / transcriptome analysis

Outline of Final Research Achievements

Genetic and endocrine control of insect hypermetamorphosis was examined for Epicauta gorhami. Larvae showed a long-day photoperiodic response at 25ºC when the food condition is regulated; larvae directly pupated under long-day conditions whereas they enter pseudopupal diapause under short-day conditions. RNA-seq analysis of the larvae that were distined to pupate or enter diapause indicated that gene expression profiles of some genes known as keys for metamorphosis in model insects are different between them.

Research Products

• [Journal Article] Food-availability dependent premature metamorphosis in the bean blister beetle, Epicauta gorhami (Coleoptera: Meloidae), a hypermetamorphic insect that feeds on grasshopper eggs in the larval stage. (2014)
  • Author(s): Misato Terao, Yuzuru Hirose, Yoshinori Shintani
  • Journal Title: Entomological Science Volume: 17 Issue: 1 Pages: 85-93
  • DOI: 10.1111/ens.12084
  • Peer Reviewed
• [Journal Article] 過変態昆虫マメハンミョウの生活史調節機構 (2014)
  • Author(s): 新谷喜紀
  • Journal Title: 昆虫と自然 Volume: 49 Pages: 19-22
  • NAID: 40020332476
• [Journal Article] Effects of temperature and photoperiod on termination of pseudopupal diapause in the bean blister beetle, Epicauta gorhami (2012)
  • Author(s): Misato Terao, Yuzuru Hirose, Yoshinori Shintani
  • Journal Title: Journal of Insect Physiology Volume: 58 Issue: 5 Pages: 737-724
  • DOI: 10.1016/j.jinsphys.2012.02.009
  • Peer Reviewed
• [Presentation] 過変態昆虫マメハンミョウの環境適応 (2013)
  • Author(s): 新谷喜紀
  • Organizer: 日本昆虫学会
  • Place of Presentation: 北海道大学
  • Invited
• [Presentation] 過変態昆虫マメハンミョウの生活史調節機構 (2013)
  • Author(s): 新谷喜紀
  • Organizer: 九州昆虫セミナー（日本昆虫学会九州支部会）
  • Place of Presentation: 佐賀大学
  • Invited
• [Presentation] Effects of temperature and photoperiod on hypermetamorphosis in the been blister beetle, Epicauta gorhami (2012)
  • Author(s): Shintani, Y., Terao, M. and Hirose, Y.
  • Organizer: International Congress of Entomology
  • Place of Presentation: Daegu
• [Presentation] マメハンミョウの早熟変態個体は繁殖できるのか (2012)
  • Author(s): 寺尾美里・新谷喜紀
  • Organizer: 日本昆虫学会
  • Place of Presentation: 町田
• [Presentation] マメハンミョウの擬蛹休眠終了に及ぼす温度の効果 奈良 (2012)
  • Author(s): 寺尾 美里・廣瀬 譲・新谷 喜紀
  • Organizer: 第56回日本応用動物昆虫学会大会
  • Place of Presentation: 近畿大学奈良キャンパス