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Exploration of molecular mechanisms underlying the interaction between host insects and symbiotic bacteria and development of basic technology for the application

Research Project

Project/Area Number 23580084
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied entomology
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

ANBUTSU Hisashi  独立行政法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (30392583)

Co-Investigator(Renkei-kenkyūsha) NIKOH Naruo  放送大学, 教養学部, 教授 (70364916)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords共生細菌 / ショウジョウバエ / スピロプラズマ / 生殖操作 / 雄殺し / バクテリオファージ / キイロショウジョウバエ / ファージ / P58 / カスリショウジョウバエ / 性比
Research Abstract

Some spiroplasmas cause female-biased sex ratios of their host insects including Drosophila fruit flies, as a result of selective death of the male offspring during embryogenesis. In this study, three closely related bacteriophages were isolated from two male-killing spiroplasmas and a non-male-killing one, and their whole genome sequences were determined. Each phage genome included five sequences with similarity to putative virulence genes of closely related spiroplasma. In general, density dynamics of the three phages showed different pattern. The expression levels of a gene were about 10 times higher in adult flies infected with male-killing spiroplasma than in those infected with non-male-killing one.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (12 results)

All 2014 2012 Other

All Journal Article (1 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway2014

    • Author(s)
      Toshiyuki Harumoto, Hisashi Anbutsu, Takema Fukatsu
    • Journal Title

      PLos Pathogen

      Volume: Vol.10 Issue: 2 Pages: e1003956-e1003956

    • DOI

      10.1371/journal.ppat.1003956

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] ショウジョウバエ-スピロプラズマ共生系における宿主-共生細菌間相互作用2014

    • Author(s)
      安佛尚志、春本敏之、二河成男、田中康次郎、深津武馬
    • Organizer
      第41回日本マイコプラズマ学会学術集会
    • Place of Presentation
      東京大学伊藤謝恩ホール
    • Year and Date
      2014-05-22
    • Related Report
      2013 Final Research Report
  • [Presentation] ショウジョウバエの共生細菌スピロプラズマのファージの比較ゲノム解析2014

    • Author(s)
      安佛尚志、二河成男、田中康次郎、春本敏之、深津武馬
    • Organizer
      第58回日本応用動物昆虫学会大会
    • Place of Presentation
      高知大学(高知市)
    • Year and Date
      2014-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Quantitative analysis of proliferation and gene expression of bacteriophages infecting male-killing and non-male-killing spiroplasmas in Drosophila2014

    • Author(s)
      Hisashi Anbutsu, Naruo Nikoh, Kohjiro Tanaka, Toshiyuki Harumoto, Takema Fukatsu
    • Organizer
      8th International Wolbachia Conference
    • Place of Presentation
      Innsbruck (Austria)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Exploration of host genes involved in Drosophila-Spiroplasma endosymbiosis and male-killing phenotype by using RNA-seq2012

    • Author(s)
      安佛尚志、春本敏之、重信秀治、吉武和敏、深津武馬
    • Organizer
      第10回日本ショウジョウバエ研究会
    • Place of Presentation
      東京慈恵医科大学(新橋)
    • Year and Date
      2012-10-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Comparative genomic analysis of bacteriophages associated with male-killing and non-male-killing spiroplasmas in Drosophila2012

    • Author(s)
      Hisashi Anbutsu, Naruo Nikoh, Kohjiro Tanaka, Takema Fukatsu
    • Organizer
      7th International Wolbachia Conference
    • Place of Presentation
      Oleron Island (France)
    • Year and Date
      2012-06-08
    • Related Report
      2013 Final Research Report
  • [Presentation] ショウジョウバエの共生細菌スピロプラズマのファージの比較ゲノム解析

    • Author(s)
      安佛尚志、二河成男、田中康次郎、春本敏之、深津武馬
    • Organizer
      第58回日本応用動物昆虫学会大会
    • Place of Presentation
      高知大学(高知市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ショウジョウバエ-スピロプラズマ共生系における宿主-共生細菌間相互作用

    • Author(s)
      安佛尚志、春本敏之、二河成男、田中康次郎、深津武馬
    • Organizer
      第41回日本マイコプラズマ学会学術集会
    • Place of Presentation
      東京大学伊藤謝恩ホール
    • Related Report
      2013 Annual Research Report
  • [Presentation] Comparative genomic analysis of bacteriophages associated with male-killing and non-male-killing spiroplasmas in Drosophila

    • Author(s)
      安佛 尚志、二河 成男、田中康次郎、深津 武馬
    • Organizer
      7th International Wolbachia Conference
    • Place of Presentation
      Oleron Island, France
    • Related Report
      2012 Research-status Report
  • [Presentation] Exploration of host genes involved in Drosophila-Spiroplasma endosymbiosis and male-killing phenotype by using RNA-seq

    • Author(s)
      安佛 尚志、春本 敏之、重信秀治、吉武和敏、深津 武馬
    • Organizer
      第10回日本ショウジョウバエ研究会(ポスター発表)
    • Place of Presentation
      東京慈恵会医科大学、新橋
    • Related Report
      2012 Research-status Report
  • [Presentation] Exploration of host genes involved in Drosophila-Spiroplasma endosymbiosis and male-killing phenotype by using RNA-seq

    • Author(s)
      安佛 尚志、春本 敏之、重信秀治、吉武和敏、深津 武馬
    • Organizer
      第10回日本ショウジョウバエ研究会(口頭発表)
    • Place of Presentation
      東京慈恵会医科大学、新橋
    • Related Report
      2012 Research-status Report
  • [Remarks] 産総研論文検索システム(産総研リポジトリ)

    • URL

      http://www.aist.go.jp/aist_j/aist_repository/index.html

    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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