Screening of arbuscular mycorrhizal fungi with high acid phosphatase secretion and it utilization

• Project/Area Number: 23580086
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Yamagata University
• Principal Investigator: TAWARAYA Keitaro 山形大学, 農学部, 教授 (70179919)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リン / 菌根 / ホスファターゼ / 菌根菌 / 菌糸 / 有機物

Research Abstract

Activity of acid phosphatase (ACP) from extraradical hyphae of arbuscular mycorrhizal (AM) fungi was studied in order to clarify utilization of soil organic phosphorus by AM fungi. Seedlings of Allium fistulosum were inoculated or uninoculated with AM fungi Rhizophagus clarus and were grown in pots that were separated into root and hyphal compartments with nylon net of 30 µm pore size. ACP activity of soil solution in hyphal compartment was higher in inoculated A. fistulosum than uninoculated plant. ACP activity in inoculated plant was higher at low soil P condition than that at high soil P condition. These results suggest that extraradical hyphae of AM fungi R. clarus exude ACP and its activity is induced under P deficient condition.

Research Products

• [Journal Article] Arbuscular Mycorrhizal Colonization Enhanced Early Growth of Mallotus paniculatus and Albizia saman under Nursery Conditions in East Kalimantan, Indonesia (2014)
  • Author(s): Wulandari D, Saridi S, Cheng W, Tawaraya K
  • Journal Title: International Journal of Forestry Research Volume: 36 Pages: 1-8
  • DOI: 10.1155/2014/898494
  • Peer Reviewed
• [Journal Article] Metabolite profiling of shoot extracts, root extracts, and root exudates of rice plant under phosphorus deficiency (2013)
  • Author(s): Tawaraya, K., Horie, R., Saito, A., Shinano, T., Wagatsuma, T., Saito, K., Oikawa, A
  • Journal Title: J Plant Nutr Volume: 36 Issue: 7 Pages: 1138-1159
  • DOI: 10.1080/01904167.2013.780613
  • Peer Reviewed
• [Journal Article] リン酸資源の枯渇に対応したリン栄養研究4.土壌微生物によるリン酸施肥削減 (2012)
  • Author(s): 俵谷圭太郎
  • Journal Title: 日本土壌肥料学雑誌 Volume: 83 Pages: 620-624
  • NAID: 110009593772
  • Peer Reviewed
• [Journal Article] noculation of arbuscular mycorrhizal fungi can substantially reduce phosphate fertilizer application to Allium fistulosum L. and achieve marketable yield under field condition (2012)
  • Author(s): Tawaraya, K. Hirose, R. Wagatsuma, T.
  • Journal Title: Biology and Fertility of Soils Volume: 48 Issue: 7 Pages: 839-843
  • DOI: 10.1007/s00374-012-0669-2
  • Peer Reviewed
• [Presentation] アーバスキュラー菌根菌の外生菌糸が浸出する酸性ホスファターゼ活性の低リン条件下における応答 (2013)
  • Author(s): 佐藤匠、程為国、江澤辰広、俵谷圭太郎
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2013-09-11
• [Presentation] Detection of acid phosphatase activity in the rhizosphere of arbuscular mycorrhizal plant (2013)
  • Author(s): Sato, T. Matsuda, K. Ezawa, T. Cheng, W. Tawaraya, K
  • Organizer: 7th International Conference of Mycorrhiza
  • Place of Presentation: India
• [Presentation] Detection of acid phosphatase activity in the rhizosphere of arbuscular mycorrhizal plan (2013)
  • Author(s): Sato T, Matsuda K, Ezawa T, Cheng W, Tawaraya K
  • Organizer: 7 th International conference of Mycorrhiza.
  • Place of Presentation: India, India Habitat Center
• [Presentation] Metabolite profiling of shoot extracts, root extracts, and root exudates of rice under phosphorus deficiency (2012)
  • Author(s): Tawaraya, K
  • Organizer: 8th Symposium of the International Society of Root Research
  • Place of Presentation: Dundee, UK
• [Presentation] 菌根形成した植物の根圏における酸性ホスファターゼ活性 (2011)
  • Author(s): 佐藤匠、松田健一郎、江澤辰広、程為国、俵谷圭太郎
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: つくば市
  • Year and Date: 2011-08-08
• [Presentation] 菌根形成した植物の根圏における酸性ホスファターゼ活性
  • Author(s): 佐藤匠、松田健一郎、江沢辰広、程為国、俵谷圭太郎
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: つくば市
• [Book] アーバスキュラー菌根、菌類の事典 (2013)
  • Author(s): 俵谷圭太郎
  • Total Pages: 308
  • Publisher: 朝倉書店