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Coning and characterization of nitrate absorption- and assimilation-related genes in tea plant

Research Project

Project/Area Number 23580088
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionShizuoka University

Principal Investigator

MORITA Akio  静岡大学, (連合)農学研究科(研究院), 教授 (20324337)

Co-Investigator(Kenkyū-buntansha) IKKA Takashi  静岡大学, 農学研究科, 助教 (90580647)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsチャ / 硝酸還元酵素 / 硝酸トランスポーター / 硝酸同化
Research Abstract

In this study, we isolated one of nitrate reductase (NR), one of nitrite reductase (NiR) and five nitrate transporter (CsNRT) genes from tea plant.
The transcript levels of these genes in tea plant cultivated under different nitrogen nutrient conditions were increased with increasing nitrate concentration and decreased with increasing ammonia concentration. In addition, it was found that nitrate absorption activity of tea is lower than ammonia.
These results suggested that the causes of low nitrate absorption and assimilation activity in tea plant might be due to the transcriptional processes and the nitrate transport into shoots is limited.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (21 results)

All 2014 2013 2012 2011 Other

All Journal Article (4 results) Presentation (17 results)

  • [Journal Article] Cloning and Characteri -zation of Inorganic Nitrogen Transporter of Tea Plant (Camellia sinensis L.)2014

    • Author(s)
      Ryosuke Take, Takuya Baba, Akio Morita, Takashi Ikka
    • Journal Title

      Proceedings of 5th International Conference on O-CHA (Tea) Culture and Science

      Volume: 35

    • Related Report
      2013 Final Research Report
  • [Journal Article] Cloning and Characterization of Inorganic Nitrogen Transporter of Tea Plant (Camellia sinensis L.)2014

    • Author(s)
      Ryosuke Take, Takuya Baba, Akio Morita, Takashi Ikka
    • Journal Title

      Proceedings of the 5th International Conference on O-CHA (Tea) Culture and Science

      Volume: ICOS2013

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Isolation and Characterization of Nitrate Reductase in Tea Plant (Camellia sinensis. L)2014

    • Author(s)
      Takuya Baba, Ryosuke Take, Hideyuki Katai, Yasutaka Suzuki, Akio Morita, Takashi Ikka
    • Journal Title

      Proceedings of the 5th International Conference on O-CHA (Tea) Culture and Science

      Volume: ICOS2013

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Isolation and Characteri -zation of Nitrate Reductase in Tea Plant (Camellia sinensis. L)

    • Author(s)
      Takuya Baba, Ryosuke Take, Hideyuki Katai, Yasutaka Suzuki, Akio Morita, Takashi Ikka
    • Journal Title

      Proceedings of 5th International Conference on O-CHA (Tea) Culture and Science

      Volume: 49

    • Related Report
      2013 Final Research Report
  • [Presentation] Cloning and Characteri -zation of Inorganic Nitrogen Transporter of Tea Plant (Camellia sinensis L.)2013

    • Author(s)
      Ryosuke Take, Takuya Baba, Akio Morita, Takashi Ikka
    • Organizer
      The 5th International Conference on O-CHA (Tea) Culture and Science
    • Place of Presentation
      Granship Shizuoka
    • Year and Date
      2013-11-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Isolation and Characteri -zation of Nitrate Reductase in Tea Plant (Camellia sinensis. L)2013

    • Author(s)
      Takuya Baba, Ryosuke Take, Hideyuki Katai, Yasutaka Suzuki, Akio Morita, Takashi Ikka
    • Organizer
      The 5th International Conference on O-CHA (Tea) Culture and Science
    • Place of Presentation
      Granship Shizuoka
    • Year and Date
      2013-11-07
    • Related Report
      2013 Final Research Report
  • [Presentation] チャ品種間における硝酸還元酵素遺伝子の発現特性2013

    • Author(s)
      馬場拓也,片井秀幸,鈴木康孝,一家崇志,森田明雄
    • Organizer
      日本育種学会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2013-10-13
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] チャの無機態窒素トランスポーターの単離とその発現特性の解析2013

    • Author(s)
      武亮介,一家崇志,森田明雄
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2013-09-12
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] チャの硝酸同化系酵素の発現特性の解析2013

    • Author(s)
      馬場拓也,一家崇志,森田明雄
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2013-09-12
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Cloning and Characterization of Inorganic Nitrogen Transporter of Tea Plant (Camellia sinensis L.)2013

    • Author(s)
      Ryosuke Take, Takuya Baba, Akio Morita, Takashi Ikka
    • Organizer
      The 5th International Conference on O-CHA (Tea) Culture and Science
    • Place of Presentation
      グランシップ静岡
    • Related Report
      2013 Annual Research Report
  • [Presentation] Isolation and Characterization of Nitrate Reductase in Tea Plant (Camellia sinensis. L)2013

    • Author(s)
      Takuya Baba, Ryosuke Take, Hideyuki Katai, Yasutaka Suzuki, Akio Morita, Takashi Ikka
    • Organizer
      The 5th International Conference on O-CHA (Tea) Culture and Science
    • Place of Presentation
      グランシップ静岡
    • Related Report
      2013 Annual Research Report
  • [Presentation] チャの硝酸同化系酵素遺伝子の単離とその発現特性の解析2012

    • Author(s)
      馬場拓也,一家崇志,森田明雄
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2012-09-05
    • Related Report
      2013 Final Research Report
  • [Presentation] チャの無機態窒素トランスポーターの単離とその発現特性2012

    • Author(s)
      武亮介,一家崇志,森田明雄
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2012-09-05
    • Related Report
      2013 Final Research Report
  • [Presentation] チャの硝酸還元酵素遺伝子の単離とその発現特性の解析2012

    • Author(s)
      馬場拓也,武亮介,森田明雄,一家崇志
    • Organizer
      第13回静岡ライフサイエンスシンポジウム
    • Place of Presentation
      静岡県立大学
    • Year and Date
      2012-03-17
    • Related Report
      2013 Final Research Report
  • [Presentation] チャの無機態窒素トランスポーターの単離とその発現特性2012

    • Author(s)
      武亮介,馬場拓也,森田明雄,一家崇志
    • Organizer
      第13回静岡ライフサイエンスシンポジウム
    • Place of Presentation
      静岡県立大学
    • Year and Date
      2012-03-17
    • Related Report
      2013 Final Research Report
  • [Presentation] チャの硝酸同化系酵素遺伝子の単離とその発現特性の解析2012

    • Author(s)
      馬場拓也,一家崇志,森田明雄
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      鳥取大学(鳥取県)
    • Related Report
      2012 Research-status Report
  • [Presentation] チャの無機態窒素トランスポーターの単離とその発現特性2012

    • Author(s)
      武亮介,一家崇志,森田明雄
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      鳥取大学(鳥取県)
    • Related Report
      2012 Research-status Report
  • [Presentation] チャの硝酸同化系酵素遺伝子の単離とその発現特性の解析2012

    • Author(s)
      馬場拓也、武亮介、森田明雄、一家崇志
    • Organizer
      第13回静岡ライフサイエンスシンポジウム
    • Place of Presentation
      静岡市 静岡県立大学
    • Related Report
      2011 Research-status Report
  • [Presentation] チャの無機態窒素トランスポーターの単離とその発現特性2012

    • Author(s)
      武亮介、馬場拓也、森田明雄、一家崇志
    • Organizer
      第13回静岡ライフサイエンスシンポジウム
    • Place of Presentation
      静岡市 静岡県立大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 窒素栄養条件がチャ培養細胞の硝酸還元酵素遺伝子転写量に及ぼす影響2011

    • Author(s)
      一家崇志,深谷輝美,森田明雄
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2011-08-09
    • Related Report
      2013 Final Research Report
  • [Presentation] 窒素栄養条件がチャ培養細胞の硝酸還元酵素遺伝子転写量に及ぼす影響2011

    • Author(s)
      一家崇志、深谷輝美、森田明雄
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      つくば市 つくば国際会議場
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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