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Analyses of plant responses to boron deprivation

Research Project

Project/Area Number 23580090
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionKyoto University

Principal Investigator

KOBAYASHI Masaru  京都大学, (連合)農学研究科(研究院), 准教授 (60281101)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords植物 / ホウ素 / 欠乏ストレス / 細胞壁 / ペクチン / シグナル伝達 / 無機栄養 / カルシウム / ストレス / 活性酸素
Research Abstract

Boron is an essential micronutrient for plants. Boron deficiency is one of the major constraints in crop production worldwide. However, it remains unclear how boron deficiency causes various metabolic disorders and cell death. To understand the mechanism, we analyzed the physiological changes in Arabidopsis plants induced upon boron deprivation. The cells in root elongation zone overaccumulated reactive oxygen species and died within 1 h after boron deprivation. Similar responses could be observed under calcium deprivation. Analyses suggested that the immediate responses were triggered by a failure in cross-linking the cell wall pectic polysaccharides which were newly synthesized in elongating cells.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (27 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (21 results) (of which Invited: 2 results) Remarks (3 results)

  • [Journal Article] Boron deprivation immediately causes cell death in growing roots of Arabidopsis thaliana (L.) Heynh.2013

    • Author(s)
      Yuki Oiwa, Kahori Kitayama, Masaru Kobayashi, Toru Matoh
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 59 Issue: 4 Pages: 621-627

    • DOI

      10.1080/00380768.2013.813382

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Boron nutrition of cultured tobacco BY-2 cells. VII. Rapid induction of metabolic dysfunction in cells deprived of boron as revealed by microarray analysis2013

    • Author(s)
      Koshiba T., Kobayashi M., Matsuoka K., Fujiwara T., Matoh T
    • Journal Title

      Soil Sci. Plant Nutr

      Volume: 59 Issue: 2 Pages: 189-194

    • DOI

      10.1080/00380768.2013.763383

    • NAID

      110009688309

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of Arabidopsis CTP : 3-deoxy-D-manno-2-octulosonate cytidylyltransferase (CMP-KDO synthetase), the enzyme that activates KDO during-rhamnogalacturonan II biosynthesis2011

    • Author(s)
      Kobayashi M, Kouzu N, Inami A, Toyooka K, Konishi Y, Matsuoka K, Matoh T
    • Journal Title

      Plant and Cell Physiology

      Volume: 52 Issue: 10 Pages: 1832-1843

    • DOI

      10.1093/pcp/pcr120

    • NAID

      10030304814

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] シロイヌナズナKDO-8-フォスファターゼ候補タンパク質の解析2014

    • Author(s)
      原朋美、王櫻霖、小林優、間藤徹
    • Organizer
      日本植物生理学会第55回年会
    • Place of Presentation
      富山大学(富山市)
    • Year and Date
      2014-03-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Immunocytochemical detection of rhamnogalacturonan II on forming cell plates2014

    • Author(s)
      Zhou Y., Awano T., Takabe K., Kobayashi M., Matoh T
    • Organizer
      日本植物生理学会第55回年会
    • Place of Presentation
      富山大学(富山市)
    • Year and Date
      2014-03-18
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナKDO-8-フォスファターゼ候補タンパク質の解析2014

    • Author(s)
      原朋美、王櫻霖、小林優、間藤徹
    • Organizer
      第55回 日本植物生理学会年会
    • Place of Presentation
      富山市(富山大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Immunocytochemical detection of rhamnogalacturonan II on forming cell plates2014

    • Author(s)
      Ye Zhou, Tatsuya Awano, Keiji Takabe, Masaru Kobayashi, Toru Matoh
    • Organizer
      第55回 日本植物生理学会年会
    • Place of Presentation
      富山市(富山大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ホウ素・カルシウム欠除に対するシロイヌナズナ初期応答の解2013

    • Author(s)
      北山香保理、大岩優貴、小林優、間藤徹
    • Organizer
      日本土壌肥料学会2013年度名古屋大会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2013-09-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Mechanism of early responses of plant cells to boron deprivation : possible involvement of pectic network2013

    • Author(s)
      Kitayama K., Oiwa Y., Kobayashi M., Matoh T
    • Organizer
      Boron 2013
    • Place of Presentation
      Istanbul, Turkey
    • Year and Date
      2013-08-17
    • Related Report
      2013 Final Research Report
  • [Presentation] RG-IIのKDO残基の合成に必要な酵素CTP : KDOシチジリルトランスフェラーゼ発現抑制株の解析2013

    • Author(s)
      青木亮輔、小林優、岩元明敏、松永俊朗、間藤徹
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2013-03-23
    • Related Report
      2013 Final Research Report
  • [Presentation] ホウ素・カルシウム欠除に対するシロイヌナズナ初期応答の解析2013

    • Author(s)
      北山香保理、大岩優貴、小林優、間藤徹
    • Organizer
      日本土壌肥料学会 2013年名古屋大会
    • Place of Presentation
      名古屋市(名古屋大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Mechanism of early responses of plant cells to boron deprivation: possible involvement of pectic network2013

    • Author(s)
      Kahori Kitayama, Yuki Oiwa, Masaru Kobayashi, Toru Matoh
    • Organizer
      Boron2013
    • Place of Presentation
      トルコ共和国イスタンブール市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Expression of genes involved in the synthesis of rhamnogalacturonan II-related monosaccharides in Marchantia polymorpha : Implications for the function of pectin gels in land plants2012

    • Author(s)
      Wageta M., Kobayashi M., Matoh T
    • Organizer
      Marchantia Workshop 2012
    • Place of Presentation
      ホテルグリーンピア阿蘇(熊本県阿蘇郡南阿蘇村)
    • Year and Date
      2012-11-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞壁ペクチンの生理機能―ホウ素・カルシウム栄養を手がかりとして, 植物栄養の基礎研究から見えてきた応用への可能性2012

    • Author(s)
      小林優
    • Organizer
      日本土壌肥料学会シンポジウム, 日本土壌肥料学会2012年度鳥取大会
    • Place of Presentation
      取大学(鳥取市)
    • Year and Date
      2012-09-06
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Boron nutrition in higher plants2012

    • Author(s)
      Kobayashi M., Matoh T
    • Organizer
      The Seventh International Symposium on Mineral Nutrition of Fruit Crops
    • Place of Presentation
      Chanthaburi, Thailand
    • Year and Date
      2012-05-22
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナにおけるホウ素欠乏初期応答の解析2012

    • Author(s)
      大岩優貴、北山香保理、小柴太一、小林優、間藤徹
    • Organizer
      日本植物生理学会第53回年会
    • Place of Presentation
      京都産業大学(京都市
    • Year and Date
      2012-03-17
    • Related Report
      2013 Final Research Report
  • [Presentation] 京都大学植栄研におけるホウ素研究2012

    • Author(s)
      小林優
    • Organizer
      ホウ素栄養研究会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-02-23
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞壁ペクチンの生理機能 -ホウ素・カルシウム栄養を手がかりとして-2012

    • Author(s)
      小林優
    • Organizer
      日本土壌肥料学会 2012年鳥取大会
    • Place of Presentation
      鳥取大学(鳥取県鳥取市)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] シロイヌナズナにおけるホウ素欠乏初期応答の解析2012

    • Author(s)
      大岩優貴、北山香保理、小柴太一、小林優、間藤徹
    • Organizer
      日本植物生理学会 第53回年会
    • Place of Presentation
      京都市(京都産業大学)
    • Related Report
      2011 Research-status Report
  • [Presentation] Early responses of Arabidopsis roots to boron deprivation2011

    • Author(s)
      Oiwa Y., Kitayama K., Koshiba T., Kobayashi M., Matoh T
    • Organizer
      International Symposium"Strategies of Plants against Global Environmental Change"
    • Place of Presentation
      倉敷芸文館(岡山県倉敷市)
    • Year and Date
      2011-12-08
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization of Arabidopsis CTP : KDO cytidylyltransferase, the enzyme that activates KDO during rhamnogalacturonan II biosynthesis2011

    • Author(s)
      Kobayashi M., Aoki R., Konishi Y., Toyooka K., Matoh T
    • Place of Presentation
      淡路夢舞台国際会議場(兵庫県淡路市)
    • Year and Date
      2011-10-03
    • Related Report
      2013 Final Research Report
  • [Presentation] シロイヌナズナにおけるホウ素欠乏初期応答の解析2011

    • Author(s)
      大岩優貴、小柴太一、小林優、間藤徹
    • Organizer
      日本土壌肥料学会2011年つくば大会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2011-08-08
    • Related Report
      2013 Final Research Report
  • [Presentation] Early responses of Arabidopsis roots to boron deprivation2011

    • Author(s)
      Yuki Oiwa, Kahori Kitayama, Taichi Koshiba, Toru Matoh
    • Organizer
      International Symposium "Strategies of Plants against Global Environmental Change"
    • Place of Presentation
      岡山県倉敷市(倉敷芸文館)
    • Related Report
      2011 Research-status Report
  • [Presentation] シロイヌナズナにおけるホウ素欠乏初期応答の解析2011

    • Author(s)
      大岩優貴、小柴太一、小林優、間藤徹
    • Organizer
      日本土壌肥料学会 2011年つくば大会
    • Place of Presentation
      茨城県つくば市(つくば国際会議場)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.npk.kais.kyoto-u.ac.jp

    • Related Report
      2013 Final Research Report
  • [Remarks] 京都大学 植物栄養学研究室

    • URL

      http://www.npk.kais.kyoto-u.ac.jp

    • Related Report
      2013 Annual Research Report
  • [Remarks] 京都大学 植物栄養学研究室

    • URL

      http://www.npk.kais.kyoto-u.ac.jp

    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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