Studies on novel pesticidal protein of plant laticifers by mRNA-seq and proteomic analysis.
| Project/Area Number |
23580091
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Masamitsu 京都工芸繊維大学, 工芸科学研究科, 教授 (00182460)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | クワ / 乳液 / 乳管細胞 / プロテオーム / mRNA-seq / 殺虫 / 抗菌 |
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| Research Abstract |
In this study, we hypothesized the laticifer cells in various organs of various plant species produce unique anti-insect or anti-microbial proteins and identified them by next-generation sequencing technology and proteome analysis. Then biochemical or physiological roles of these proteins were studied using native or recombinant proteins. Aim of this study is to provide new pesticidal gene, taking the place of Bt gene in transgenic crops.
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Report
(4 results)
Research Products
(10 results)
