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Studies on novel pesticidal protein of plant laticifers by mRNA-seq and proteomic analysis.

Research Project

Project/Area Number 23580091
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionKyoto Institute of Technology

Principal Investigator

KITAJIMA Sakihito  京都工芸繊維大学, 工芸科学研究科, 准教授 (70283653)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Masamitsu  京都工芸繊維大学, 工芸科学研究科, 教授 (00182460)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsクワ / 乳液 / 乳管細胞 / プロテオーム / mRNA-seq / 殺虫 / 抗菌
Research Abstract

In this study, we hypothesized the laticifer cells in various organs of various plant species produce unique anti-insect or anti-microbial proteins and identified them by next-generation sequencing technology and proteome analysis. Then biochemical or physiological roles of these proteins were studied using native or recombinant proteins. Aim of this study is to provide new pesticidal gene, taking the place of Bt gene in transgenic crops.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (10 results)

All 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Laticifers in mulberry exclusively accumulate defense proteins related to biotic stresses2013

    • Author(s)
      Sakihito KITAJIMA, Yoshihiro YAMAMOTO, Kiyoo HIROOKA, Chihaya TAKI, Susumu HIBINO
    • Journal Title

      Plant Biotechnology

      Volume: 30 Pages: 399-402

    • NAID

      10031202060

    • Related Report
      2013 Final Research Report
  • [Journal Article] A Regulatory Cascade Involving Class II ETHYLENE RESPONSE FACTOR Transcriptional Repressors Operates in the Progression of Leaf Senescence2013

    • Author(s)
      Tomotsugu Koyama, Haruka Nii, Nobutaka Mitsuda, Masaru Ohta, Sakihito Kitajima, Masaru Ohme-Takagi, Fumihiko Sato
    • Journal Title

      Plant Physiology

      Volume: 162 Pages: 991-1005

    • Related Report
      2013 Final Research Report
  • [Journal Article] Laticifers in mulberry exclusively accumulate defense proteins related to biotic stresses2013

    • Author(s)
      Sakihito KITAJIMA, Yoshihiro YAMAMOTO, Kiyoo HIROOKA, Chihaya TAKI, Susumu HIBINO
    • Journal Title

      Plant Biotechnology

      Volume: 30 Issue: 4 Pages: 399-402

    • DOI

      10.5511/plantbiotechnology.13.0326a

    • NAID

      10031202060

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Laticifers in mulberry exclusively accumulate defense proteins related to biotic stresses2013

    • Author(s)
      Sakihito KITAJIMA, Yoshihiro YAMAMOTO, Kiyoo HIROOKA, Chihaya TAKI, Susumu HIBINO
    • Journal Title

      Plant Biotechnology

      Volume: --

    • NAID

      10031202060

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparative study of gene expression and major proteins'function of laticifers in lignified and unlignified organs of mulberry2012

    • Author(s)
      Sakihito Kitajima, Toki Taira, Kenji Oda, Katsuyuki T. Yamato, Yoshihiro Inukai, Yusuke Hori
    • Journal Title

      Planta

      Volume: Volume 235, Number 3 Pages: 589-601

    • Related Report
      2013 Final Research Report
  • [Journal Article] Comparative Study of Gene Expression and Major Proteins’ Function of Laticifers in Lignified and Unlignified Organs of Mulberry2012

    • Author(s)
      Sakihito Kitajima, Toki Taira, Kenji Oda, Katsuyuki T. Yamato, Yoshihiro Inukai, and Yusuke Hori
    • Journal Title

      Planta

      Volume: 235 Issue: 3 Pages: 589-601

    • DOI

      10.1007/s00425-011-1533-6

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] クワとイチジクの乳管細胞に蓄積するmRNAとタンパク質群の器官間比較2014

    • Author(s)
      日比野進1・瀧智早1・矢野晴菜1・廣岡青央2・山本佳宏2・北島佐紀人1(1京都工繊大・2京都市産技研)
    • Organizer
      平成26年度蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      日本大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物の乳管細胞の機能を主要タンパク質成分から探る2013

    • Author(s)
      北島佐紀人
    • Organizer
      第29回植物バイテクシンポジウム
    • Place of Presentation
      京都府立大学
    • Related Report
      2013 Final Research Report
  • [Presentation] クワの乳液に蓄積するmRNAとタンパク質群の器官間比較2012

    • Author(s)
      日比野進1・瀧智早1・廣岡青央2・山本佳宏2・北島佐紀人1(1京都工繊大・2京都市産技研)
    • Organizer
      日本蚕糸学会合同支部大会
    • Place of Presentation
      信州大学
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://ameblo.jp/shokubutsu-bunshi-kit/entry-11186578754.html

    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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